Standardized Assessments of Neutralizing Antibodies for HIV/AIDS Vaccine Development

David C. Montefiori, Duke University Medical Center


This site provides detailed information for making standardized assessments of neutralizing antibodies against HIV, SIV and SHIV using molecularly cloned Env-pseudotyped viruses in an optimized and validated luciferase (Luc) reporter gene assay. Access is provided to assay protocols and macros currently in use at the Duke Central Reference Laboratory. This site also contains educational slides, tutorials and links to relevant publications and assay reagents. Special attention is given to HIV-1 reference strains that are available as molecularly cloned Env-pseudotyped viruses.

Assay Protocol and Support Documents:

Key Publications:

Reference Reagents:

Please send questions, comments and suggestions to Kelli Greene at:
We thank the Bill & Melinda Gates Foundation and their support of the
Collaboration for AIDS Vaccine Discovery (CAVD) — a component of the Global HIV Vaccine Enterprise.

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