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Displaying record number 62302

HXB2 Location  Gag(203-212)   Gag Epitope Map
View variants at this location
Epitope ETINEEAAEW   Epitope Alignment
Variants
ETINdEAAEW{DRl}   diminished response; escape documented in this paper; observed variant; processing
ETINdEAAEW{DRV}   observed variant; replicative capacity reduced; susceptible form
ETINEEAAEW{DRl}   observed variant; replicative capacity is not abrogated; susceptible form
Epitope Name EW10
Species (MHC/HLA human(A*25)

Variant Details

Showing all: 3 variant(s).


Variant ID.  4405
Epitope Seq.  ETINEEAAEW{DRV}
Variant Seq.  ETINdEAAEW{DRl}
Mutations V/L E/D
Epitope Location V13L E5D
HXB2 Location V215L E207D
Mutation Type DR: diminished response
E: escape documented in this paper
OV: observed variant
P: processing
Epitope Subtype B
Variant Subtype B
Method Longitudinal study, Other, Sequence
Note Mutant ETINdEAAEW, was seen in patient PIC1362 580 DPS (days post symptoms) onwards but not at 769 DPS. It carried an escape at E207D and with significant fitness loss; when in tandem with an extra-epitopic mutation, V215L, 3 residues downstream of EW10. In patient 1483, it was seen at 2025 DPS as the double mutant E207D intra-EW10 and V215L extra-EW10 epitope. Of note - Patient 1362's CTL response was 10x that of Patient 1483! Patients PIC1052 and PIC1349 did not carry this mutation and PIC1052 carried the consensus L at position 215 extra-EW10 epitope. Data suggest that V215L augments cleavage of EW10, decreasing its presentation and increasing its escape function.


Variant ID.  4406
Epitope Seq.  ETINEEAAEW{DRV}
Variant Seq.  ETINdEAAEW{DRV}
Mutations E/D
Epitope Location E5D
HXB2 Location E207D
Mutation Type OV: observed variant
RCR: replicative capacity reduced
SF: susceptible form
Epitope Subtype B
Variant Subtype B
Method Longitudinal study, Other, Sequence
Note Mutant ETINdEAAEW, not accompanying a downstream V215L i.e. with 215V, was seen rarely in patient PIC1362 and was not an escape. Ex-vivo studies showed that E207D alone had a loss of fitness but it was less significant than the double mutant (E207D,V215L).


Variant ID.  4407
Epitope Seq.  ETINEEAAEW{DRV}
Variant Seq.  ETINEEAAEW{DRl}
Mutations V/L
Epitope Location V13L
HXB2 Location V215L
Mutation Type OV: observed variant
RCOK: replicative capacity is not abrogated
SF: susceptible form
Epitope Subtype B
Variant Subtype B
Method Longitudinal study, Other, Sequence
Note Variant V215L is actually the HIV-B strain consensus position but was not autologous in these studies. V215L had a greater fitness in ex-vivo studies than the autologous 215V. It was seen in patients 1362 at 769 DPS onwards, patient PIC1349 between 44 and 342 DPS, and PIC1052 between 184 and 754 DPS.

References

Troyer2009 Ryan M. Troyer, John McNevin, Yi Liu, Shao Chong Zhang, Randall W. Krizan, Awet Abraha, Denis M. Tebit, Hong Zhao, Santiago Avila, Michael A. Lobritz, M. Juliana McElrath, Sylvie Le Gall, James I. Mullins, and Eric J. Arts. Variable Fitness Impact of HIV-1 Escape Mutations to Cytotoxic T Lymphocyte (CTL) Response. PLoS Pathog., 5(4):e1000365, Apr 2009. PubMed ID: 19343217. Show all entries for this paper.


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