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Found 1 matching record:

Displaying record number 62883

HXB2 Location  Pol(838-847)   Pol Epitope Map
View variants at this location
Epitope SAAVKAACWW   Epitope Alignment
Variants
{tNFT}SAAVKAACWW   replicative capacity is not abrogated
{rNFT}SAAVKAACWW   replicative capacity is not abrogated
{pNFT}SAAVKAACWW   replicative capacity reduced
SnAVKAACWW   replicative capacity reduced
SAAVKAACWW{AGIk}   replicative capacity is not abrogated
{tNFT}SttVKAACWW   replicative capacity is not abrogated
{rNFT}SttVKAACWW   replicative capacity reduced
{pNFT}SttVKAACWW   replicative capacity is not abrogated
Species (MHC/HLA human

Variant Details

Showing all: 8 variant(s).


Variant ID.  5065
Epitope Seq.  {SNFT}SAAVKAACWW
Variant Seq.  {tNFT}SAAVKAACWW
Mutations S/T
Epitope Location S-4T
HXB2 Location S834T
Mutation Type RCOK: replicative capacity is not abrogated
Epitope Subtype C
Variant Subtype C
Method Other, Relative replication capacity assay
Note The introduction of S119T (integrase protein position) onto either wild-type NL4-3 subtype B virus encoding well-defined epitope {SNFT}SttVKAACWW (see CTL variant record #5070) or recombinant NL4-3 virus with subtype C RT-integrase encoding analogous {SNFT}SAAVKAACWW had no effect on replicative capacity. Residue 119 is also located within epitope HTDNGSNF.


Variant ID.  5066
Epitope Seq.  {SNFT}SAAVKAACWW
Variant Seq.  {rNFT}SAAVKAACWW
Mutations S/R
Epitope Location S-4R
HXB2 Location S834R
Mutation Type RCOK: replicative capacity is not abrogated
Epitope Subtype C
Variant Subtype C
Method Other, Relative replication capacity assay
Note The introduction of S119R (integrase protein position) onto subtype B wild-type NL4-3 virus carrying best-defined epitope {SNFT}SttVKAACWW did not produce detectable cultured virus (see CTL variant record #5071), but did not affect replicative capacity of NL4-3 recombinant virus encoding subtype C RT-integrase carrying the analogous sequence {SNFT}SAAVKAACWW. Residue 119 is also located within epitope HTDNGSNF.


Variant ID.  5067
Epitope Seq.  {SNFT}SAAVKAACWW
Variant Seq.  {pNFT}SAAVKAACWW
Mutations S/P
Epitope Location S-4P
HXB2 Location S834P
Mutation Type RCR: replicative capacity reduced
Epitope Subtype C
Variant Subtype C
Method Other, Relative replication capacity assay
Note The introduction of S119P (integrase protein position) onto subtype B wild-type NL4-3 virus carrying best-defined epitope {SNFT}SttVKAACWW did not affect replicative capacity (see CTL variant record #5072), but did not produce detectable cultured virus on a NL4-3 recombinant virus encoding subtype C RT-integrase carrying the analogous sequence {SNFT}SAAVKAACWW. Residue 119 is also located within epitope HTDNGSNF.


Variant ID.  5068
Epitope Seq.  SAAVKAACWW
Variant Seq.  SnAVKAACWW
Mutations A/N
Epitope Location A2N
HXB2 Location A839N
Mutation Type RCR: replicative capacity reduced
Epitope Subtype C
Variant Subtype C
Method Other, Relative replication capacity assay
Note Relative to NL4-3 recombinant virus encoding subtype C wildtype SK-278 RT-integrase, analogous virus encoding integrase variant SnAVKAACWW had a RC reduction of ˜23%. A124N (integrase protein position) also flanks epitope HTDNGSNF.


Variant ID.  5069
Epitope Seq.  SAAVKAACWW{AGIQ}
Variant Seq.  SAAVKAACWW{AGIk}
Mutations Q/K
Epitope Location Q+4K
HXB2 Location Q851K
Mutation Type RCOK: replicative capacity is not abrogated
Epitope Subtype C
Variant Subtype C
Method Other, Relative replication capacity assay
Note Relative to subtype B wild-type NL4-3 virus and NL4-3 recombinant virus encoding subtype C wildtype SK-278 RT-integrase, analogous viruses encoding integrase variant SAAVKAACWW{AGIk} did not mediate a significant reduction in RC. Q136K (integrase protein position) is also located within nearby epitope IQQEFGIPY.


Variant ID.  5070
Epitope Seq.  {SNFT}SAAVKAACWW
Variant Seq.  {tNFT}SttVKAACWW
Mutations A/T A/T S/T
Epitope Location A2T A3T S-4T
HXB2 Location A839T A840T S834T
Mutation Type RCOK: replicative capacity is not abrogated
Epitope Subtype B
Variant Subtype B
Method Other, Relative replication capacity assay
Note The introduction of S119T (integrase protein position) onto either wild-type NL4-3 subtype B virus encoding well-defined epitope {SNFT}SttVKAACWW or recombinant NL4-3 virus with subtype C RT-integrase encoding analogous {SNFT}SAAVKAACWW (see CTL variant record #5065) had no effect on replicative capacity. Residue 119 is also located within epitope HTDNGSNF.


Variant ID.  5071
Epitope Seq.  {SNFT}SAAVKAACWW
Variant Seq.  {rNFT}SttVKAACWW
Mutations A/T A/T S/R
Epitope Location A2T A3T S-4R
HXB2 Location A839T A840T S834R
Mutation Type RCR: replicative capacity reduced
Epitope Subtype B
Variant Subtype B
Method Other, Relative replication capacity assay
Note The introduction of S119R (integrase protein position) onto subtype B wild-type NL4-3 virus carrying best-defined epitope {SNFT}SttVKAACWW did not produce detectable cultured virus, but did not affect replicative capacity of NL4-3 recombinant virus encoding subtype C RT-integrase carrying the analogous sequence {SNFT}SAAVKAACWW (see CTL variant record #5066). Residue 119 is also located within epitope HTDNGSNF.


Variant ID.  5072
Epitope Seq.  {SNFT}SAAVKAACWW
Variant Seq.  {pNFT}SttVKAACWW
Mutations A/T A/T S/P
Epitope Location A2T A3T S-4P
HXB2 Location A839T A840T S834P
Mutation Type RCOK: replicative capacity is not abrogated
Epitope Subtype B
Variant Subtype B
Method Other, Relative replication capacity assay
Note The introduction of S119P (integrase protein position) onto subtype B wild-type NL4-3 virus carrying best-defined epitope {SNFT}SttVKAACWW did not affect replicative capacity, but did not produce detectable cultured virus on a NL4-3 recombinant virus encoding subtype C RT-integrase carrying the analogous sequence {SNFT}SAAVKAACWW (see CTL variant record #5067). Residue 119 is also located within epitope HTDNGSNF.

References

Ojwach2021 Doty B. A. Ojwach, Paradise Madlala, Michelle Gordon, Thumbi Ndung'u, and Jaclyn K. Mann. Vulnerable targets in HIV-1 Pol for attenuation-based vaccine design. Virology, 554:1-8 doi, Feb 2021. PubMed ID: 33316731 Show all entries for this paper.


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