HIV molecular immunology database
Found 1 matching record:
| HXB2 Location | Pol(283-290) | Pol Epitope Map
View variants at this location |
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| Epitope |
TAFTIPSI
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Epitope Alignment | |||||||||||||||||||
| Variants |
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| Species (MHC/HLA) | human(B51) | ||||||||||||||||||||
Showing all: 6 variant(s).
| Variant ID. | 1254 |
|---|---|
| Epitope Seq. | TAFTIPSI |
| Variant Seq. | TAFTIPSt |
| Mutations | I/T |
| Epitope Location | I8T |
| HXB2 Location | I290T |
| Mutation Type | SF: susceptible form |
| Method | Chromium-release assay |
| Note | This variant has slightly altered CTL recognition and lysis, but 100x higher peptide concentration was required for 50% half maximum lysis. [Menendez-Arias1998]. |
| Variant ID. | 1255 |
|---|---|
| Epitope Seq. | TAFTIPSI |
| Variant Seq. | TAFTIPSv |
| Mutations | I/V |
| Epitope Location | I8V |
| HXB2 Location | I290V |
| Mutation Type | SF: susceptible form |
| Method | Chromium-release assay |
| Note | This variant has slightly altered CTL recognition and lysis, but 10x higher peptide concentration was required for 50% half maximum lysis. [Menendez-Arias1998]. |
| Variant ID. | 1256 |
|---|---|
| Epitope Seq. | TAFTIPSI |
| Variant Seq. | TvFTIPSI |
| Mutations | A/V |
| Epitope Location | A2V |
| HXB2 Location | A284V |
| Mutation Type | NSF: non-susceptible form |
| Method | Chromium-release assay |
| Note | CTL recognition of this highly conserved Ala-129 is almost abrogated. |
| Variant ID. | 1619 |
|---|---|
| Epitope Seq. | TAFTIPSI |
| Variant Seq. | TAFTIPSt |
| Mutations | I/T |
| Epitope Location | I8T |
| HXB2 Location | I290T |
| Mutation Type | DR: diminished response |
| Epitope Subtype | B |
| Variant Subtype | B |
| Method | Chromium-release assay |
| Note | This naturally occurring, strain CAMI variant, was poorly recognized by a Patient LWS CTL clone. [Sipsas1997]. |
| Variant ID. | 1620 |
|---|---|
| Epitope Seq. | TAFTIPSI |
| Variant Seq. | TAFTIPSv |
| Mutations | I/V |
| Epitope Location | I8V |
| HXB2 Location | I290V |
| Mutation Type | DR: diminished response |
| Epitope Subtype | B |
| Variant Subtype | B |
| Method | Chromium-release assay |
| Note | This naturally occurring, strain CAMI variant, was not well recognized by a Patient LWS CTL clone. [Sipsas1997]. |
| Variant ID. | 1621 |
|---|---|
| Epitope Seq. | TAFTIPSI |
| Variant Seq. | TvFTIPSt |
| Mutations | A/V I/T |
| Epitope Location | A2V I8T |
| HXB2 Location | A284V I290T |
| Mutation Type | DR: diminished response |
| Epitope Subtype | B |
| Variant Subtype | B |
| Method | Chromium-release assay |
| Note | This naturally occurring, strain MANC variant, was very poorly recognized by a Patient LWS CTL clone. |
Menendez-Arias1998 L. Menendez-Arias, A. Mas, and E. Domingo. Cytotoxic T-Lymphocyte Responses to HIV-1 Reverse Transcriptase. Viral Immunol., 11:167-181, 1998. PubMed ID: 10189185. Show all entries for this paper.
Sipsas1997 N. V. Sipsas, S. A. Kalams, A. Trocha, S. He, W. A. Blattner, B. D. Walker, and R. P. Johnson. Identification of Type-Specific Cytotoxic T Lymphocyte Responses to Homologous Viral Proteins in Laboratory Workers Accidentally Infected with HIV-1. J. Clin. Invest., 99:752-762, 1997. To examine a situation where the autologous strain and the reference reagents would be the same, the CTL response of three lab workers accidentally infected with HIV IIIB was studied. Both group specific and type specific epitopes were targets for CTL clones. One subject had a broadening of CTL response over time, using a broad range of restricting HLA class I alleles. Characterization of the cytotoxic T lymphocyte (CTL) response against HIV-1 has been limited by the use of target cells expressing viral proteins from laboratory isolates of HIV-1. This approach has favored identification of group-specific CTL responses and precluded assessment of the extent of type-specific CTL responses directed against HIV-1. Using cells expressing viral proteins from the HIV-1 IIIB strain, we performed a detailed characterization of HIV-1-specific CTL response in three laboratory workers accidentally infected with HIV-1 IIIB. Eight of the epitopes identified were group specific, lying in relatively conserved regions of Gag, reverse transcriptase, and envelope. Three type-specific epitopes were identified, two of them in highly variable regions of envelope. In longitudinal studies in one subject, seven different epitopes and five different restricting HLA class I alleles were identified, with a progressive increase in the number of CTL epitopes recognized by this subject over time. Our data demonstrate that type-specific CTL responses make up a significant proportion of the host cellular immune response against HIV-1 and that a broadening of epitope specificity may occur. PubMed ID: 9045880. Show all entries for this paper.