HIV molecular immunology database

 

Search the Epitope Variant and Escape Mutation Database

Help


Found 1 matching record:

Displaying record number 434

HXB2 Location  Pol(283-290)   Pol Epitope Map
View variants at this location
Epitope TAFTIPSI   Epitope Alignment
Variants
TAFTIPSt   susceptible form
TAFTIPSv   susceptible form
TvFTIPSI   non-susceptible form
TAFTIPSt   diminished response
TAFTIPSv   diminished response
TvFTIPSt   diminished response
Species (MHC/HLA human(B51)

Variant Details

Showing all: 6 variant(s).


Variant ID.  1254
Epitope Seq.  TAFTIPSI
Variant Seq.  TAFTIPSt
Mutations I/T
Epitope Location I8T
HXB2 Location I290T
Mutation Type SF: susceptible form
Method Chromium-release assay
Note This variant has slightly altered CTL recognition and lysis, but 100x higher peptide concentration was required for 50% half maximum lysis. [Menendez-Arias1998].


Variant ID.  1255
Epitope Seq.  TAFTIPSI
Variant Seq.  TAFTIPSv
Mutations I/V
Epitope Location I8V
HXB2 Location I290V
Mutation Type SF: susceptible form
Method Chromium-release assay
Note This variant has slightly altered CTL recognition and lysis, but 10x higher peptide concentration was required for 50% half maximum lysis. [Menendez-Arias1998].


Variant ID.  1256
Epitope Seq.  TAFTIPSI
Variant Seq.  TvFTIPSI
Mutations A/V
Epitope Location A2V
HXB2 Location A284V
Mutation Type NSF: non-susceptible form
Method Chromium-release assay
Note CTL recognition of this highly conserved Ala-129 is almost abrogated.


Variant ID.  1619
Epitope Seq.  TAFTIPSI
Variant Seq.  TAFTIPSt
Mutations I/T
Epitope Location I8T
HXB2 Location I290T
Mutation Type DR: diminished response
Epitope Subtype B
Variant Subtype B
Method Chromium-release assay
Note This naturally occurring, strain CAMI variant, was poorly recognized by a Patient LWS CTL clone. [Sipsas1997].


Variant ID.  1620
Epitope Seq.  TAFTIPSI
Variant Seq.  TAFTIPSv
Mutations I/V
Epitope Location I8V
HXB2 Location I290V
Mutation Type DR: diminished response
Epitope Subtype B
Variant Subtype B
Method Chromium-release assay
Note This naturally occurring, strain CAMI variant, was not well recognized by a Patient LWS CTL clone. [Sipsas1997].


Variant ID.  1621
Epitope Seq.  TAFTIPSI
Variant Seq.  TvFTIPSt
Mutations A/V I/T
Epitope Location A2V I8T
HXB2 Location A284V I290T
Mutation Type DR: diminished response
Epitope Subtype B
Variant Subtype B
Method Chromium-release assay
Note This naturally occurring, strain MANC variant, was very poorly recognized by a Patient LWS CTL clone.

References

Menendez-Arias1998 L. Menendez-Arias, A. Mas, and E. Domingo. Cytotoxic T-Lymphocyte Responses to HIV-1 Reverse Transcriptase. Viral Immunol., 11:167-181, 1998. PubMed ID: 10189185. Show all entries for this paper.

Sipsas1997 N. V. Sipsas, S. A. Kalams, A. Trocha, S. He, W. A. Blattner, B. D. Walker, and R. P. Johnson. Identification of Type-Specific Cytotoxic T Lymphocyte Responses to Homologous Viral Proteins in Laboratory Workers Accidentally Infected with HIV-1. J. Clin. Invest., 99:752-762, 1997. To examine a situation where the autologous strain and the reference reagents would be the same, the CTL response of three lab workers accidentally infected with HIV IIIB was studied. Both group specific and type specific epitopes were targets for CTL clones. One subject had a broadening of CTL response over time, using a broad range of restricting HLA class I alleles. Characterization of the cytotoxic T lymphocyte (CTL) response against HIV-1 has been limited by the use of target cells expressing viral proteins from laboratory isolates of HIV-1. This approach has favored identification of group-specific CTL responses and precluded assessment of the extent of type-specific CTL responses directed against HIV-1. Using cells expressing viral proteins from the HIV-1 IIIB strain, we performed a detailed characterization of HIV-1-specific CTL response in three laboratory workers accidentally infected with HIV-1 IIIB. Eight of the epitopes identified were group specific, lying in relatively conserved regions of Gag, reverse transcriptase, and envelope. Three type-specific epitopes were identified, two of them in highly variable regions of envelope. In longitudinal studies in one subject, seven different epitopes and five different restricting HLA class I alleles were identified, with a progressive increase in the number of CTL epitopes recognized by this subject over time. Our data demonstrate that type-specific CTL responses make up a significant proportion of the host cellular immune response against HIV-1 and that a broadening of epitope specificity may occur. PubMed ID: 9045880. Show all entries for this paper.


Questions or comments? Contact us at immuno@lanl.gov
 
Managed by Triad National Security, LLC for the U.S. Department of Energy’s National Nuclear Security Administration
Copyright © 2022 Triad National Security, LLC | Disclaimer/Privacy

Dept of Health & Human Services Los Alamos National Institutes of Health