HIV molecular immunology database
Found 9 matching records:
| HXB2 Location | Gag(77-85) | Gag Epitope Map
View variants at this location |
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|---|---|---|---|---|---|---|---|---|---|
| Epitope |
SLYNTVATL
|
Epitope Alignment | |||||||
| Variants |
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| Epitope Name | SL9 | ||||||||
| Species (MHC/HLA) | human(A2) | ||||||||
Showing all: 2 variant(s).
| Variant ID. | 1622 |
|---|---|
| Epitope Seq. | SLYNTVATL |
| Variant Seq. | SLYNTVAvL |
| Mutations | T/V |
| Epitope Location | T8V |
| HXB2 Location | T84V |
| Mutation Type | SF: susceptible form |
| Epitope Subtype | B |
| Variant Subtype | B |
| Method | Chromium-release assay |
| Note | This naturally occurring, strain MANC variant, was recognized by a Patient LWF CTL clone. |
| Variant ID. | 1623 |
|---|---|
| Epitope Seq. | SLYNTVATL |
| Variant Seq. | SLfNTVAvL |
| Mutations | Y/F T/V |
| Epitope Location | Y3F T8V |
| HXB2 Location | Y79F T84V |
| Mutation Type | SF: susceptible form |
| Epitope Subtype | B |
| Variant Subtype | B |
| Method | Chromium-release assay |
| Note | This naturally occurring, strain NY5CG variant, was recognized by a Patient LWF CTL clone. |
Sipsas1997 N. V. Sipsas, S. A. Kalams, A. Trocha, S. He, W. A. Blattner, B. D. Walker, and R. P. Johnson. Identification of Type-Specific Cytotoxic T Lymphocyte Responses to Homologous Viral Proteins in Laboratory Workers Accidentally Infected with HIV-1. J. Clin. Invest., 99:752-762, 1997. To examine a situation where the autologous strain and the reference reagents would be the same, the CTL response of three lab workers accidentally infected with HIV IIIB was studied. Both group specific and type specific epitopes were targets for CTL clones. One subject had a broadening of CTL response over time, using a broad range of restricting HLA class I alleles. Characterization of the cytotoxic T lymphocyte (CTL) response against HIV-1 has been limited by the use of target cells expressing viral proteins from laboratory isolates of HIV-1. This approach has favored identification of group-specific CTL responses and precluded assessment of the extent of type-specific CTL responses directed against HIV-1. Using cells expressing viral proteins from the HIV-1 IIIB strain, we performed a detailed characterization of HIV-1-specific CTL response in three laboratory workers accidentally infected with HIV-1 IIIB. Eight of the epitopes identified were group specific, lying in relatively conserved regions of Gag, reverse transcriptase, and envelope. Three type-specific epitopes were identified, two of them in highly variable regions of envelope. In longitudinal studies in one subject, seven different epitopes and five different restricting HLA class I alleles were identified, with a progressive increase in the number of CTL epitopes recognized by this subject over time. Our data demonstrate that type-specific CTL responses make up a significant proportion of the host cellular immune response against HIV-1 and that a broadening of epitope specificity may occur. PubMed ID: 9045880. Show all entries for this paper.
| HXB2 Location | Gag(215-224) | Gag Epitope Map
View variants at this location |
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| Epitope |
VHPVHAGPIA
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Epitope Alignment | |||||||||||||
| Variants |
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| Species (MHC/HLA) | human(B55) | ||||||||||||||
Showing all: 4 variant(s).
| Variant ID. | 1624 |
|---|---|
| Epitope Seq. | VHPVHAGPIA |
| Variant Seq. | lHPVHAGPvA |
| Mutations | V/L I/V |
| Epitope Location | V1L I9V |
| HXB2 Location | V215L I223V |
| Mutation Type | SF: susceptible form |
| Epitope Subtype | B |
| Variant Subtype | B |
| Method | Chromium-release assay |
| Note | This naturally occurring, strain PH136 variant, was recognized by a Patient LWR CTL clone. |
| Variant ID. | 1625 |
|---|---|
| Epitope Seq. | VHPVHAGPIA |
| Variant Seq. | lHPVHAGPIA |
| Mutations | V/L |
| Epitope Location | V1L |
| HXB2 Location | V215L |
| Mutation Type | SF: susceptible form |
| Epitope Subtype | B |
| Variant Subtype | B |
| Method | Chromium-release assay |
| Note | This naturally occurring, strain RF variant, was recognized by a Patient LWR CTL clone. |
| Variant ID. | 1626 |
|---|---|
| Epitope Seq. | VHPVHAGPIA |
| Variant Seq. | lHPVHAGPIt |
| Mutations | V/L A/T |
| Epitope Location | V1L A10T |
| HXB2 Location | V215L A224T |
| Mutation Type | SF: susceptible form |
| Epitope Subtype | B |
| Variant Subtype | B |
| Method | Chromium-release assay |
| Note | This naturally occurring, strain MN variant, was recognized by a Patient LWR CTL clone. |
| Variant ID. | 1627 |
|---|---|
| Epitope Seq. | VHPVHAGPIA |
| Variant Seq. | lHPaqAGPIA |
| Mutations | V/L V/A H/Q |
| Epitope Location | V1L V4A H5Q |
| HXB2 Location | V215L V218A H219Q |
| Mutation Type | SF: susceptible form |
| Epitope Subtype | B |
| Variant Subtype | B |
| Method | Chromium-release assay |
| Note | This naturally occurring, strain JH3 variant, was very poorly recognized by a Patient LWR CTL clone. |
Sipsas1997 N. V. Sipsas, S. A. Kalams, A. Trocha, S. He, W. A. Blattner, B. D. Walker, and R. P. Johnson. Identification of Type-Specific Cytotoxic T Lymphocyte Responses to Homologous Viral Proteins in Laboratory Workers Accidentally Infected with HIV-1. J. Clin. Invest., 99:752-762, 1997. To examine a situation where the autologous strain and the reference reagents would be the same, the CTL response of three lab workers accidentally infected with HIV IIIB was studied. Both group specific and type specific epitopes were targets for CTL clones. One subject had a broadening of CTL response over time, using a broad range of restricting HLA class I alleles. Characterization of the cytotoxic T lymphocyte (CTL) response against HIV-1 has been limited by the use of target cells expressing viral proteins from laboratory isolates of HIV-1. This approach has favored identification of group-specific CTL responses and precluded assessment of the extent of type-specific CTL responses directed against HIV-1. Using cells expressing viral proteins from the HIV-1 IIIB strain, we performed a detailed characterization of HIV-1-specific CTL response in three laboratory workers accidentally infected with HIV-1 IIIB. Eight of the epitopes identified were group specific, lying in relatively conserved regions of Gag, reverse transcriptase, and envelope. Three type-specific epitopes were identified, two of them in highly variable regions of envelope. In longitudinal studies in one subject, seven different epitopes and five different restricting HLA class I alleles were identified, with a progressive increase in the number of CTL epitopes recognized by this subject over time. Our data demonstrate that type-specific CTL responses make up a significant proportion of the host cellular immune response against HIV-1 and that a broadening of epitope specificity may occur. PubMed ID: 9045880. Show all entries for this paper.
| HXB2 Location | Gag(349-359) | Gag Epitope Map
View variants at this location |
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|---|---|---|---|---|---|---|
| Epitope |
ACQGVGGPGHK
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Epitope Alignment | ||||
| Variants |
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| Species (MHC/HLA) | human(A11) | |||||
Showing all: 1 variant(s).
| Variant ID. | 1628 |
|---|---|
| Epitope Seq. | ACQGVGGPGHK |
| Variant Seq. | ACQGVGGPsHK |
| Mutations | G/S |
| Epitope Location | G9S |
| HXB2 Location | G357S |
| Mutation Type | SF: susceptible form |
| Epitope Subtype | B |
| Variant Subtype | B |
| Method | Chromium-release assay |
| Note | This naturally occurring, strain RF variant, was recognized by a Patient LWF CTL clone. |
Sipsas1997 N. V. Sipsas, S. A. Kalams, A. Trocha, S. He, W. A. Blattner, B. D. Walker, and R. P. Johnson. Identification of Type-Specific Cytotoxic T Lymphocyte Responses to Homologous Viral Proteins in Laboratory Workers Accidentally Infected with HIV-1. J. Clin. Invest., 99:752-762, 1997. To examine a situation where the autologous strain and the reference reagents would be the same, the CTL response of three lab workers accidentally infected with HIV IIIB was studied. Both group specific and type specific epitopes were targets for CTL clones. One subject had a broadening of CTL response over time, using a broad range of restricting HLA class I alleles. Characterization of the cytotoxic T lymphocyte (CTL) response against HIV-1 has been limited by the use of target cells expressing viral proteins from laboratory isolates of HIV-1. This approach has favored identification of group-specific CTL responses and precluded assessment of the extent of type-specific CTL responses directed against HIV-1. Using cells expressing viral proteins from the HIV-1 IIIB strain, we performed a detailed characterization of HIV-1-specific CTL response in three laboratory workers accidentally infected with HIV-1 IIIB. Eight of the epitopes identified were group specific, lying in relatively conserved regions of Gag, reverse transcriptase, and envelope. Three type-specific epitopes were identified, two of them in highly variable regions of envelope. In longitudinal studies in one subject, seven different epitopes and five different restricting HLA class I alleles were identified, with a progressive increase in the number of CTL epitopes recognized by this subject over time. Our data demonstrate that type-specific CTL responses make up a significant proportion of the host cellular immune response against HIV-1 and that a broadening of epitope specificity may occur. PubMed ID: 9045880. Show all entries for this paper.
| HXB2 Location | Pol(273-282) | Pol Epitope Map
View variants at this location |
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|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Epitope |
VPLDEDFRKY
|
Epitope Alignment | ||||||||||
| Variants |
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| Species (MHC/HLA) | human(B35) | |||||||||||
Showing all: 3 variant(s).
| Variant ID. | 1253 |
|---|---|
| Epitope Seq. | VPLDEDFRKY |
| Variant Seq. | VPLhEDFRKY |
| Mutations | D/H |
| Epitope Location | D4H |
| HXB2 Location | D276H |
| Mutation Type | NSF: non-susceptible form |
| Method | Chromium-release assay |
| Note | This variant was not recognized by CTL generated against the Glu-122 epitope, VPLDEDFRKY. |
| Variant ID. | 1615 |
|---|---|
| Epitope Seq. | VPLDEDFRKY |
| Variant Seq. | VPLDkDFRKY |
| Mutations | E/K |
| Epitope Location | E5K |
| HXB2 Location | E277K |
| Mutation Type | NSF: non-susceptible form |
| Epitope Subtype | B |
| Variant Subtype | B |
| Method | Chromium-release assay |
| Note | This naturally occurring, strain MN variant, was not recognized by a Patient LWF CTL clone. |
| Variant ID. | 1616 |
|---|---|
| Epitope Seq. | VPLDEDFRKY |
| Variant Seq. | VPhDEDFRKY |
| Mutations | L/H |
| Epitope Location | L3H |
| HXB2 Location | L275H |
| Mutation Type | NSF: non-susceptible form |
| Epitope Subtype | B |
| Variant Subtype | B |
| Method | Chromium-release assay |
| Note | This naturally occurring, strain YU2 variant, was not recognized by a Patient LWF CTL clone. |
Sipsas1997 N. V. Sipsas, S. A. Kalams, A. Trocha, S. He, W. A. Blattner, B. D. Walker, and R. P. Johnson. Identification of Type-Specific Cytotoxic T Lymphocyte Responses to Homologous Viral Proteins in Laboratory Workers Accidentally Infected with HIV-1. J. Clin. Invest., 99:752-762, 1997. To examine a situation where the autologous strain and the reference reagents would be the same, the CTL response of three lab workers accidentally infected with HIV IIIB was studied. Both group specific and type specific epitopes were targets for CTL clones. One subject had a broadening of CTL response over time, using a broad range of restricting HLA class I alleles. Characterization of the cytotoxic T lymphocyte (CTL) response against HIV-1 has been limited by the use of target cells expressing viral proteins from laboratory isolates of HIV-1. This approach has favored identification of group-specific CTL responses and precluded assessment of the extent of type-specific CTL responses directed against HIV-1. Using cells expressing viral proteins from the HIV-1 IIIB strain, we performed a detailed characterization of HIV-1-specific CTL response in three laboratory workers accidentally infected with HIV-1 IIIB. Eight of the epitopes identified were group specific, lying in relatively conserved regions of Gag, reverse transcriptase, and envelope. Three type-specific epitopes were identified, two of them in highly variable regions of envelope. In longitudinal studies in one subject, seven different epitopes and five different restricting HLA class I alleles were identified, with a progressive increase in the number of CTL epitopes recognized by this subject over time. Our data demonstrate that type-specific CTL responses make up a significant proportion of the host cellular immune response against HIV-1 and that a broadening of epitope specificity may occur. PubMed ID: 9045880. Show all entries for this paper.
| HXB2 Location | Pol(283-290) | Pol Epitope Map
View variants at this location |
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|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Epitope |
TAFTIPSI
|
Epitope Alignment | |||||||||||||||||||
| Variants |
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| Species (MHC/HLA) | human(B51) | ||||||||||||||||||||
Showing all: 6 variant(s).
| Variant ID. | 1254 |
|---|---|
| Epitope Seq. | TAFTIPSI |
| Variant Seq. | TAFTIPSt |
| Mutations | I/T |
| Epitope Location | I8T |
| HXB2 Location | I290T |
| Mutation Type | SF: susceptible form |
| Method | Chromium-release assay |
| Note | This variant has slightly altered CTL recognition and lysis, but 100x higher peptide concentration was required for 50% half maximum lysis. [Menendez-Arias1998]. |
| Variant ID. | 1255 |
|---|---|
| Epitope Seq. | TAFTIPSI |
| Variant Seq. | TAFTIPSv |
| Mutations | I/V |
| Epitope Location | I8V |
| HXB2 Location | I290V |
| Mutation Type | SF: susceptible form |
| Method | Chromium-release assay |
| Note | This variant has slightly altered CTL recognition and lysis, but 10x higher peptide concentration was required for 50% half maximum lysis. [Menendez-Arias1998]. |
| Variant ID. | 1256 |
|---|---|
| Epitope Seq. | TAFTIPSI |
| Variant Seq. | TvFTIPSI |
| Mutations | A/V |
| Epitope Location | A2V |
| HXB2 Location | A284V |
| Mutation Type | NSF: non-susceptible form |
| Method | Chromium-release assay |
| Note | CTL recognition of this highly conserved Ala-129 is almost abrogated. |
| Variant ID. | 1619 |
|---|---|
| Epitope Seq. | TAFTIPSI |
| Variant Seq. | TAFTIPSt |
| Mutations | I/T |
| Epitope Location | I8T |
| HXB2 Location | I290T |
| Mutation Type | DR: diminished response |
| Epitope Subtype | B |
| Variant Subtype | B |
| Method | Chromium-release assay |
| Note | This naturally occurring, strain CAMI variant, was poorly recognized by a Patient LWS CTL clone. [Sipsas1997]. |
| Variant ID. | 1620 |
|---|---|
| Epitope Seq. | TAFTIPSI |
| Variant Seq. | TAFTIPSv |
| Mutations | I/V |
| Epitope Location | I8V |
| HXB2 Location | I290V |
| Mutation Type | DR: diminished response |
| Epitope Subtype | B |
| Variant Subtype | B |
| Method | Chromium-release assay |
| Note | This naturally occurring, strain CAMI variant, was not well recognized by a Patient LWS CTL clone. [Sipsas1997]. |
| Variant ID. | 1621 |
|---|---|
| Epitope Seq. | TAFTIPSI |
| Variant Seq. | TvFTIPSt |
| Mutations | A/V I/T |
| Epitope Location | A2V I8T |
| HXB2 Location | A284V I290T |
| Mutation Type | DR: diminished response |
| Epitope Subtype | B |
| Variant Subtype | B |
| Method | Chromium-release assay |
| Note | This naturally occurring, strain MANC variant, was very poorly recognized by a Patient LWS CTL clone. |
Menendez-Arias1998 L. Menendez-Arias, A. Mas, and E. Domingo. Cytotoxic T-Lymphocyte Responses to HIV-1 Reverse Transcriptase. Viral Immunol., 11:167-181, 1998. PubMed ID: 10189185. Show all entries for this paper.
Sipsas1997 N. V. Sipsas, S. A. Kalams, A. Trocha, S. He, W. A. Blattner, B. D. Walker, and R. P. Johnson. Identification of Type-Specific Cytotoxic T Lymphocyte Responses to Homologous Viral Proteins in Laboratory Workers Accidentally Infected with HIV-1. J. Clin. Invest., 99:752-762, 1997. To examine a situation where the autologous strain and the reference reagents would be the same, the CTL response of three lab workers accidentally infected with HIV IIIB was studied. Both group specific and type specific epitopes were targets for CTL clones. One subject had a broadening of CTL response over time, using a broad range of restricting HLA class I alleles. Characterization of the cytotoxic T lymphocyte (CTL) response against HIV-1 has been limited by the use of target cells expressing viral proteins from laboratory isolates of HIV-1. This approach has favored identification of group-specific CTL responses and precluded assessment of the extent of type-specific CTL responses directed against HIV-1. Using cells expressing viral proteins from the HIV-1 IIIB strain, we performed a detailed characterization of HIV-1-specific CTL response in three laboratory workers accidentally infected with HIV-1 IIIB. Eight of the epitopes identified were group specific, lying in relatively conserved regions of Gag, reverse transcriptase, and envelope. Three type-specific epitopes were identified, two of them in highly variable regions of envelope. In longitudinal studies in one subject, seven different epitopes and five different restricting HLA class I alleles were identified, with a progressive increase in the number of CTL epitopes recognized by this subject over time. Our data demonstrate that type-specific CTL responses make up a significant proportion of the host cellular immune response against HIV-1 and that a broadening of epitope specificity may occur. PubMed ID: 9045880. Show all entries for this paper.
| HXB2 Location | Pol(330-338) | Pol Epitope Map
View variants at this location |
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|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Epitope |
NPDIVIYQY
|
Epitope Alignment | ||||||||||||||||
| Variants |
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| Species (MHC/HLA) | human(B35) | |||||||||||||||||
Showing all: 5 variant(s).
| Variant ID. | 1266 |
|---|---|
| Epitope Seq. | NPDIVIYQY |
| Variant Seq. | hPDIVIYQY |
| Mutations | N/H |
| Epitope Location | N1H |
| HXB2 Location | N330H |
| Mutation Type | SF: susceptible form |
| Epitope Subtype | B |
| Variant Subtype | A |
| Note | This variant is the HIV-1 Clade A consensus and obtained by a Medline database search in August 1998. |
| Variant ID. | 1267 |
|---|---|
| Epitope Seq. | NPDIVIYQY |
| Variant Seq. | hPDIlIYQY |
| Mutations | N/H V/L |
| Epitope Location | N1H V5L |
| HXB2 Location | N330H V334L |
| Mutation Type | SF: susceptible form |
| Epitope Subtype | B |
| Note | This HIV-2 homologous epitope is recognized by NPDIVIYQY-specific clones. |
| Variant ID. | 1629 |
|---|---|
| Epitope Seq. | NPDIVIYQY |
| Variant Seq. | NPDIiIYQY |
| Mutations | V/I |
| Epitope Location | V5I |
| HXB2 Location | V334I |
| Mutation Type | SF: susceptible form |
| Epitope Subtype | B |
| Variant Subtype | B |
| Method | Chromium-release assay |
| Note | This naturally occurring, strain JRCSF variant, was recognized by a Patient LWF CTL clone. |
| Variant ID. | 1630 |
|---|---|
| Epitope Seq. | NPDIVIYQY |
| Variant Seq. | NPeIVIYQY |
| Mutations | D/E |
| Epitope Location | D3E |
| HXB2 Location | D332E |
| Mutation Type | SF: susceptible form |
| Epitope Subtype | B |
| Variant Subtype | B |
| Method | Chromium-release assay |
| Note | This naturally occurring, strain JRU2RF variant, was recognized by a Patient LWF CTL clone. |
| Variant ID. | 1631 |
|---|---|
| Epitope Seq. | NPDIVIYQY |
| Variant Seq. | NPDlVIYQY |
| Mutations | I/L |
| Epitope Location | I4L |
| HXB2 Location | I333L |
| Mutation Type | SF: susceptible form |
| Epitope Subtype | B |
| Variant Subtype | B |
| Method | Chromium-release assay |
| Note | This naturally occurring variant was recognized by a Patient LWF CTL clone. |
Menendez-Arias1998 L. Menendez-Arias, A. Mas, and E. Domingo. Cytotoxic T-Lymphocyte Responses to HIV-1 Reverse Transcriptase. Viral Immunol., 11:167-181, 1998. PubMed ID: 10189185. Show all entries for this paper.
Sipsas1997 N. V. Sipsas, S. A. Kalams, A. Trocha, S. He, W. A. Blattner, B. D. Walker, and R. P. Johnson. Identification of Type-Specific Cytotoxic T Lymphocyte Responses to Homologous Viral Proteins in Laboratory Workers Accidentally Infected with HIV-1. J. Clin. Invest., 99:752-762, 1997. To examine a situation where the autologous strain and the reference reagents would be the same, the CTL response of three lab workers accidentally infected with HIV IIIB was studied. Both group specific and type specific epitopes were targets for CTL clones. One subject had a broadening of CTL response over time, using a broad range of restricting HLA class I alleles. Characterization of the cytotoxic T lymphocyte (CTL) response against HIV-1 has been limited by the use of target cells expressing viral proteins from laboratory isolates of HIV-1. This approach has favored identification of group-specific CTL responses and precluded assessment of the extent of type-specific CTL responses directed against HIV-1. Using cells expressing viral proteins from the HIV-1 IIIB strain, we performed a detailed characterization of HIV-1-specific CTL response in three laboratory workers accidentally infected with HIV-1 IIIB. Eight of the epitopes identified were group specific, lying in relatively conserved regions of Gag, reverse transcriptase, and envelope. Three type-specific epitopes were identified, two of them in highly variable regions of envelope. In longitudinal studies in one subject, seven different epitopes and five different restricting HLA class I alleles were identified, with a progressive increase in the number of CTL epitopes recognized by this subject over time. Our data demonstrate that type-specific CTL responses make up a significant proportion of the host cellular immune response against HIV-1 and that a broadening of epitope specificity may occur. PubMed ID: 9045880. Show all entries for this paper.
| HXB2 Location | gp160(2-10) | gp160 Epitope Map
View variants at this location |
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|---|---|---|---|---|---|---|
| Epitope |
RVKEKYQHL
|
Epitope Alignment | ||||
| Variants |
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| Species (MHC/HLA) | human(B8) | |||||
Showing all: 1 variant(s).
| Variant ID. | 1617 |
|---|---|
| Epitope Seq. | RVKEKYQHL |
| Variant Seq. | RVKgirknYQHL |
| Mutations | E/G K/I Y/R Q/K H/N L/Y |
| Epitope Location | E4G K5I Y6R Q7K H8N L9Y |
| HXB2 Location | E5G K6I Y7R Q8K H9N L10Y |
| Mutation Type | NSF: non-susceptible form |
| Epitope Subtype | B |
| Variant Subtype | B |
| Method | Chromium-release assay |
| Note | This naturally occurring, strain JRCSF variant, was not recognized by a Patient LWS CTL clone. |
Sipsas1997 N. V. Sipsas, S. A. Kalams, A. Trocha, S. He, W. A. Blattner, B. D. Walker, and R. P. Johnson. Identification of Type-Specific Cytotoxic T Lymphocyte Responses to Homologous Viral Proteins in Laboratory Workers Accidentally Infected with HIV-1. J. Clin. Invest., 99:752-762, 1997. To examine a situation where the autologous strain and the reference reagents would be the same, the CTL response of three lab workers accidentally infected with HIV IIIB was studied. Both group specific and type specific epitopes were targets for CTL clones. One subject had a broadening of CTL response over time, using a broad range of restricting HLA class I alleles. Characterization of the cytotoxic T lymphocyte (CTL) response against HIV-1 has been limited by the use of target cells expressing viral proteins from laboratory isolates of HIV-1. This approach has favored identification of group-specific CTL responses and precluded assessment of the extent of type-specific CTL responses directed against HIV-1. Using cells expressing viral proteins from the HIV-1 IIIB strain, we performed a detailed characterization of HIV-1-specific CTL response in three laboratory workers accidentally infected with HIV-1 IIIB. Eight of the epitopes identified were group specific, lying in relatively conserved regions of Gag, reverse transcriptase, and envelope. Three type-specific epitopes were identified, two of them in highly variable regions of envelope. In longitudinal studies in one subject, seven different epitopes and five different restricting HLA class I alleles were identified, with a progressive increase in the number of CTL epitopes recognized by this subject over time. Our data demonstrate that type-specific CTL responses make up a significant proportion of the host cellular immune response against HIV-1 and that a broadening of epitope specificity may occur. PubMed ID: 9045880. Show all entries for this paper.
| HXB2 Location | gp160(156-165) | gp160 Epitope Map
View variants at this location |
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|---|---|---|---|---|---|---|
| Epitope |
NCSFNISTSI
|
Epitope Alignment | ||||
| Variants |
|
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| Species (MHC/HLA) | human(Cw8) | |||||
Showing all: 1 variant(s).
| Variant ID. | 1618 |
|---|---|
| Epitope Seq. | NCSFNISTSI |
| Variant Seq. | NCSFNItTSI |
| Mutations | S/T |
| Epitope Location | S7T |
| HXB2 Location | S162T |
| Mutation Type | NSF: non-susceptible form |
| Epitope Subtype | B |
| Variant Subtype | B |
| Method | Chromium-release assay |
| Note | This naturally occurring, strain MN variant, was not recognized by a Patient LWF CTL clone. |
Sipsas1997 N. V. Sipsas, S. A. Kalams, A. Trocha, S. He, W. A. Blattner, B. D. Walker, and R. P. Johnson. Identification of Type-Specific Cytotoxic T Lymphocyte Responses to Homologous Viral Proteins in Laboratory Workers Accidentally Infected with HIV-1. J. Clin. Invest., 99:752-762, 1997. To examine a situation where the autologous strain and the reference reagents would be the same, the CTL response of three lab workers accidentally infected with HIV IIIB was studied. Both group specific and type specific epitopes were targets for CTL clones. One subject had a broadening of CTL response over time, using a broad range of restricting HLA class I alleles. Characterization of the cytotoxic T lymphocyte (CTL) response against HIV-1 has been limited by the use of target cells expressing viral proteins from laboratory isolates of HIV-1. This approach has favored identification of group-specific CTL responses and precluded assessment of the extent of type-specific CTL responses directed against HIV-1. Using cells expressing viral proteins from the HIV-1 IIIB strain, we performed a detailed characterization of HIV-1-specific CTL response in three laboratory workers accidentally infected with HIV-1 IIIB. Eight of the epitopes identified were group specific, lying in relatively conserved regions of Gag, reverse transcriptase, and envelope. Three type-specific epitopes were identified, two of them in highly variable regions of envelope. In longitudinal studies in one subject, seven different epitopes and five different restricting HLA class I alleles were identified, with a progressive increase in the number of CTL epitopes recognized by this subject over time. Our data demonstrate that type-specific CTL responses make up a significant proportion of the host cellular immune response against HIV-1 and that a broadening of epitope specificity may occur. PubMed ID: 9045880. Show all entries for this paper.
| HXB2 Location | gp160(557-565) | gp160 Epitope Map
View variants at this location |
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|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Epitope |
RAIEAQQHL
|
Epitope Alignment | |||||||||||||
| Variants |
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| Species (MHC/HLA) | human(B51) | ||||||||||||||
Showing all: 4 variant(s).
| Variant ID. | 1632 |
|---|---|
| Epitope Seq. | RAIEAQQHL |
| Variant Seq. | kAIEAQQHL |
| Mutations | R/K |
| Epitope Location | R1K |
| HXB2 Location | R557K |
| Mutation Type | SF: susceptible form |
| Epitope Subtype | B |
| Variant Subtype | B |
| Method | Chromium-release assay |
| Note | This naturally occurring, strain NY5CG variant, was recognized by a Patient LWS CTL clone. |
| Variant ID. | 1633 |
|---|---|
| Epitope Seq. | RAIEAQQHL |
| Variant Seq. | RAIEAQQHm |
| Mutations | L/M |
| Epitope Location | L9M |
| HXB2 Location | L565M |
| Mutation Type | SF: susceptible form |
| Epitope Subtype | B |
| Variant Subtype | B |
| Method | Chromium-release assay |
| Note | This naturally occurring, strain JRCSF variant, was recognized by a Patient LWS CTL clone. |
| Variant ID. | 1634 |
|---|---|
| Epitope Seq. | RAIEAQQHL |
| Variant Seq. | RAIdAQQHL |
| Mutations | E/D |
| Epitope Location | E4D |
| HXB2 Location | E560D |
| Mutation Type | SF: susceptible form |
| Epitope Subtype | B |
| Variant Subtype | B |
| Method | Chromium-release assay |
| Note | This naturally occurring, strain ETR variant, was recognized by a Patient LWS CTL clone. |
| Variant ID. | 1635 |
|---|---|
| Epitope Seq. | RAIEAQQHL |
| Variant Seq. | RAIkAQQHL |
| Mutations | E/K |
| Epitope Location | E4K |
| HXB2 Location | E560K |
| Mutation Type | SF: susceptible form |
| Epitope Subtype | B |
| Variant Subtype | B |
| Method | Chromium-release assay |
| Note | This naturally occurring, strain CDC42 variant, was recognized by a Patient LWS CTL clone. |
Sipsas1997 N. V. Sipsas, S. A. Kalams, A. Trocha, S. He, W. A. Blattner, B. D. Walker, and R. P. Johnson. Identification of Type-Specific Cytotoxic T Lymphocyte Responses to Homologous Viral Proteins in Laboratory Workers Accidentally Infected with HIV-1. J. Clin. Invest., 99:752-762, 1997. To examine a situation where the autologous strain and the reference reagents would be the same, the CTL response of three lab workers accidentally infected with HIV IIIB was studied. Both group specific and type specific epitopes were targets for CTL clones. One subject had a broadening of CTL response over time, using a broad range of restricting HLA class I alleles. Characterization of the cytotoxic T lymphocyte (CTL) response against HIV-1 has been limited by the use of target cells expressing viral proteins from laboratory isolates of HIV-1. This approach has favored identification of group-specific CTL responses and precluded assessment of the extent of type-specific CTL responses directed against HIV-1. Using cells expressing viral proteins from the HIV-1 IIIB strain, we performed a detailed characterization of HIV-1-specific CTL response in three laboratory workers accidentally infected with HIV-1 IIIB. Eight of the epitopes identified were group specific, lying in relatively conserved regions of Gag, reverse transcriptase, and envelope. Three type-specific epitopes were identified, two of them in highly variable regions of envelope. In longitudinal studies in one subject, seven different epitopes and five different restricting HLA class I alleles were identified, with a progressive increase in the number of CTL epitopes recognized by this subject over time. Our data demonstrate that type-specific CTL responses make up a significant proportion of the host cellular immune response against HIV-1 and that a broadening of epitope specificity may occur. PubMed ID: 9045880. Show all entries for this paper.