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Found 9 matching records:

Displaying record number 82

HXB2 Location  Gag(77-85)   Gag Epitope Map
View variants at this location
Epitope SLYNTVATL   Epitope Alignment
Variants
SLYNTVAvL   susceptible form
SLfNTVAvL   susceptible form
Epitope Name SL9
Species (MHC/HLA human(A2)

Variant Details

Showing all: 2 variant(s).


Variant ID.  1622
Epitope Seq.  SLYNTVATL
Variant Seq.  SLYNTVAvL
Mutations T/V
Epitope Location T8V
HXB2 Location T84V
Mutation Type SF: susceptible form
Epitope Subtype B
Variant Subtype B
Method Chromium-release assay
Note This naturally occurring, strain MANC variant, was recognized by a Patient LWF CTL clone.


Variant ID.  1623
Epitope Seq.  SLYNTVATL
Variant Seq.  SLfNTVAvL
Mutations Y/F T/V
Epitope Location Y3F T8V
HXB2 Location Y79F T84V
Mutation Type SF: susceptible form
Epitope Subtype B
Variant Subtype B
Method Chromium-release assay
Note This naturally occurring, strain NY5CG variant, was recognized by a Patient LWF CTL clone.

References

Sipsas1997 N. V. Sipsas, S. A. Kalams, A. Trocha, S. He, W. A. Blattner, B. D. Walker, and R. P. Johnson. Identification of Type-Specific Cytotoxic T Lymphocyte Responses to Homologous Viral Proteins in Laboratory Workers Accidentally Infected with HIV-1. J. Clin. Invest., 99:752-762, 1997. To examine a situation where the autologous strain and the reference reagents would be the same, the CTL response of three lab workers accidentally infected with HIV IIIB was studied. Both group specific and type specific epitopes were targets for CTL clones. One subject had a broadening of CTL response over time, using a broad range of restricting HLA class I alleles. Characterization of the cytotoxic T lymphocyte (CTL) response against HIV-1 has been limited by the use of target cells expressing viral proteins from laboratory isolates of HIV-1. This approach has favored identification of group-specific CTL responses and precluded assessment of the extent of type-specific CTL responses directed against HIV-1. Using cells expressing viral proteins from the HIV-1 IIIB strain, we performed a detailed characterization of HIV-1-specific CTL response in three laboratory workers accidentally infected with HIV-1 IIIB. Eight of the epitopes identified were group specific, lying in relatively conserved regions of Gag, reverse transcriptase, and envelope. Three type-specific epitopes were identified, two of them in highly variable regions of envelope. In longitudinal studies in one subject, seven different epitopes and five different restricting HLA class I alleles were identified, with a progressive increase in the number of CTL epitopes recognized by this subject over time. Our data demonstrate that type-specific CTL responses make up a significant proportion of the host cellular immune response against HIV-1 and that a broadening of epitope specificity may occur. PubMed ID: 9045880. Show all entries for this paper.


Displaying record number 214

HXB2 Location  Gag(215-224)   Gag Epitope Map
View variants at this location
Epitope VHPVHAGPIA   Epitope Alignment
Variants
lHPVHAGPvA   susceptible form
lHPVHAGPIA   susceptible form
lHPVHAGPIt   susceptible form
lHPaqAGPIA   susceptible form
Species (MHC/HLA human(B55)

Variant Details

Showing all: 4 variant(s).


Variant ID.  1624
Epitope Seq.  VHPVHAGPIA
Variant Seq.  lHPVHAGPvA
Mutations V/L I/V
Epitope Location V1L I9V
HXB2 Location V215L I223V
Mutation Type SF: susceptible form
Epitope Subtype B
Variant Subtype B
Method Chromium-release assay
Note This naturally occurring, strain PH136 variant, was recognized by a Patient LWR CTL clone.


Variant ID.  1625
Epitope Seq.  VHPVHAGPIA
Variant Seq.  lHPVHAGPIA
Mutations V/L
Epitope Location V1L
HXB2 Location V215L
Mutation Type SF: susceptible form
Epitope Subtype B
Variant Subtype B
Method Chromium-release assay
Note This naturally occurring, strain RF variant, was recognized by a Patient LWR CTL clone.


Variant ID.  1626
Epitope Seq.  VHPVHAGPIA
Variant Seq.  lHPVHAGPIt
Mutations V/L A/T
Epitope Location V1L A10T
HXB2 Location V215L A224T
Mutation Type SF: susceptible form
Epitope Subtype B
Variant Subtype B
Method Chromium-release assay
Note This naturally occurring, strain MN variant, was recognized by a Patient LWR CTL clone.


Variant ID.  1627
Epitope Seq.  VHPVHAGPIA
Variant Seq.  lHPaqAGPIA
Mutations V/L V/A H/Q
Epitope Location V1L V4A H5Q
HXB2 Location V215L V218A H219Q
Mutation Type SF: susceptible form
Epitope Subtype B
Variant Subtype B
Method Chromium-release assay
Note This naturally occurring, strain JH3 variant, was very poorly recognized by a Patient LWR CTL clone.

References

Sipsas1997 N. V. Sipsas, S. A. Kalams, A. Trocha, S. He, W. A. Blattner, B. D. Walker, and R. P. Johnson. Identification of Type-Specific Cytotoxic T Lymphocyte Responses to Homologous Viral Proteins in Laboratory Workers Accidentally Infected with HIV-1. J. Clin. Invest., 99:752-762, 1997. To examine a situation where the autologous strain and the reference reagents would be the same, the CTL response of three lab workers accidentally infected with HIV IIIB was studied. Both group specific and type specific epitopes were targets for CTL clones. One subject had a broadening of CTL response over time, using a broad range of restricting HLA class I alleles. Characterization of the cytotoxic T lymphocyte (CTL) response against HIV-1 has been limited by the use of target cells expressing viral proteins from laboratory isolates of HIV-1. This approach has favored identification of group-specific CTL responses and precluded assessment of the extent of type-specific CTL responses directed against HIV-1. Using cells expressing viral proteins from the HIV-1 IIIB strain, we performed a detailed characterization of HIV-1-specific CTL response in three laboratory workers accidentally infected with HIV-1 IIIB. Eight of the epitopes identified were group specific, lying in relatively conserved regions of Gag, reverse transcriptase, and envelope. Three type-specific epitopes were identified, two of them in highly variable regions of envelope. In longitudinal studies in one subject, seven different epitopes and five different restricting HLA class I alleles were identified, with a progressive increase in the number of CTL epitopes recognized by this subject over time. Our data demonstrate that type-specific CTL responses make up a significant proportion of the host cellular immune response against HIV-1 and that a broadening of epitope specificity may occur. PubMed ID: 9045880. Show all entries for this paper.


Displaying record number 360

HXB2 Location  Gag(349-359)   Gag Epitope Map
View variants at this location
Epitope ACQGVGGPGHK   Epitope Alignment
Variants
ACQGVGGPsHK   susceptible form
Species (MHC/HLA human(A11)

Variant Details

Showing all: 1 variant(s).


Variant ID.  1628
Epitope Seq.  ACQGVGGPGHK
Variant Seq.  ACQGVGGPsHK
Mutations G/S
Epitope Location G9S
HXB2 Location G357S
Mutation Type SF: susceptible form
Epitope Subtype B
Variant Subtype B
Method Chromium-release assay
Note This naturally occurring, strain RF variant, was recognized by a Patient LWF CTL clone.

References

Sipsas1997 N. V. Sipsas, S. A. Kalams, A. Trocha, S. He, W. A. Blattner, B. D. Walker, and R. P. Johnson. Identification of Type-Specific Cytotoxic T Lymphocyte Responses to Homologous Viral Proteins in Laboratory Workers Accidentally Infected with HIV-1. J. Clin. Invest., 99:752-762, 1997. To examine a situation where the autologous strain and the reference reagents would be the same, the CTL response of three lab workers accidentally infected with HIV IIIB was studied. Both group specific and type specific epitopes were targets for CTL clones. One subject had a broadening of CTL response over time, using a broad range of restricting HLA class I alleles. Characterization of the cytotoxic T lymphocyte (CTL) response against HIV-1 has been limited by the use of target cells expressing viral proteins from laboratory isolates of HIV-1. This approach has favored identification of group-specific CTL responses and precluded assessment of the extent of type-specific CTL responses directed against HIV-1. Using cells expressing viral proteins from the HIV-1 IIIB strain, we performed a detailed characterization of HIV-1-specific CTL response in three laboratory workers accidentally infected with HIV-1 IIIB. Eight of the epitopes identified were group specific, lying in relatively conserved regions of Gag, reverse transcriptase, and envelope. Three type-specific epitopes were identified, two of them in highly variable regions of envelope. In longitudinal studies in one subject, seven different epitopes and five different restricting HLA class I alleles were identified, with a progressive increase in the number of CTL epitopes recognized by this subject over time. Our data demonstrate that type-specific CTL responses make up a significant proportion of the host cellular immune response against HIV-1 and that a broadening of epitope specificity may occur. PubMed ID: 9045880. Show all entries for this paper.


Displaying record number 431

HXB2 Location  Pol(273-282)   Pol Epitope Map
View variants at this location
Epitope VPLDEDFRKY   Epitope Alignment
Variants
VPLhEDFRKY   non-susceptible form
VPLDkDFRKY   non-susceptible form
VPhDEDFRKY   non-susceptible form
Species (MHC/HLA human(B35)

Variant Details

Showing all: 3 variant(s).


Variant ID.  1253
Epitope Seq.  VPLDEDFRKY
Variant Seq.  VPLhEDFRKY
Mutations D/H
Epitope Location D4H
HXB2 Location D276H
Mutation Type NSF: non-susceptible form
Method Chromium-release assay
Note This variant was not recognized by CTL generated against the Glu-122 epitope, VPLDEDFRKY.


Variant ID.  1615
Epitope Seq.  VPLDEDFRKY
Variant Seq.  VPLDkDFRKY
Mutations E/K
Epitope Location E5K
HXB2 Location E277K
Mutation Type NSF: non-susceptible form
Epitope Subtype B
Variant Subtype B
Method Chromium-release assay
Note This naturally occurring, strain MN variant, was not recognized by a Patient LWF CTL clone.


Variant ID.  1616
Epitope Seq.  VPLDEDFRKY
Variant Seq.  VPhDEDFRKY
Mutations L/H
Epitope Location L3H
HXB2 Location L275H
Mutation Type NSF: non-susceptible form
Epitope Subtype B
Variant Subtype B
Method Chromium-release assay
Note This naturally occurring, strain YU2 variant, was not recognized by a Patient LWF CTL clone.

References

Sipsas1997 N. V. Sipsas, S. A. Kalams, A. Trocha, S. He, W. A. Blattner, B. D. Walker, and R. P. Johnson. Identification of Type-Specific Cytotoxic T Lymphocyte Responses to Homologous Viral Proteins in Laboratory Workers Accidentally Infected with HIV-1. J. Clin. Invest., 99:752-762, 1997. To examine a situation where the autologous strain and the reference reagents would be the same, the CTL response of three lab workers accidentally infected with HIV IIIB was studied. Both group specific and type specific epitopes were targets for CTL clones. One subject had a broadening of CTL response over time, using a broad range of restricting HLA class I alleles. Characterization of the cytotoxic T lymphocyte (CTL) response against HIV-1 has been limited by the use of target cells expressing viral proteins from laboratory isolates of HIV-1. This approach has favored identification of group-specific CTL responses and precluded assessment of the extent of type-specific CTL responses directed against HIV-1. Using cells expressing viral proteins from the HIV-1 IIIB strain, we performed a detailed characterization of HIV-1-specific CTL response in three laboratory workers accidentally infected with HIV-1 IIIB. Eight of the epitopes identified were group specific, lying in relatively conserved regions of Gag, reverse transcriptase, and envelope. Three type-specific epitopes were identified, two of them in highly variable regions of envelope. In longitudinal studies in one subject, seven different epitopes and five different restricting HLA class I alleles were identified, with a progressive increase in the number of CTL epitopes recognized by this subject over time. Our data demonstrate that type-specific CTL responses make up a significant proportion of the host cellular immune response against HIV-1 and that a broadening of epitope specificity may occur. PubMed ID: 9045880. Show all entries for this paper.


Displaying record number 434

HXB2 Location  Pol(283-290)   Pol Epitope Map
View variants at this location
Epitope TAFTIPSI   Epitope Alignment
Variants
TAFTIPSt   susceptible form
TAFTIPSv   susceptible form
TvFTIPSI   non-susceptible form
TAFTIPSt   diminished response
TAFTIPSv   diminished response
TvFTIPSt   diminished response
Species (MHC/HLA human(B51)

Variant Details

Showing all: 6 variant(s).


Variant ID.  1254
Epitope Seq.  TAFTIPSI
Variant Seq.  TAFTIPSt
Mutations I/T
Epitope Location I8T
HXB2 Location I290T
Mutation Type SF: susceptible form
Method Chromium-release assay
Note This variant has slightly altered CTL recognition and lysis, but 100x higher peptide concentration was required for 50% half maximum lysis. [Menendez-Arias1998].


Variant ID.  1255
Epitope Seq.  TAFTIPSI
Variant Seq.  TAFTIPSv
Mutations I/V
Epitope Location I8V
HXB2 Location I290V
Mutation Type SF: susceptible form
Method Chromium-release assay
Note This variant has slightly altered CTL recognition and lysis, but 10x higher peptide concentration was required for 50% half maximum lysis. [Menendez-Arias1998].


Variant ID.  1256
Epitope Seq.  TAFTIPSI
Variant Seq.  TvFTIPSI
Mutations A/V
Epitope Location A2V
HXB2 Location A284V
Mutation Type NSF: non-susceptible form
Method Chromium-release assay
Note CTL recognition of this highly conserved Ala-129 is almost abrogated.


Variant ID.  1619
Epitope Seq.  TAFTIPSI
Variant Seq.  TAFTIPSt
Mutations I/T
Epitope Location I8T
HXB2 Location I290T
Mutation Type DR: diminished response
Epitope Subtype B
Variant Subtype B
Method Chromium-release assay
Note This naturally occurring, strain CAMI variant, was poorly recognized by a Patient LWS CTL clone. [Sipsas1997].


Variant ID.  1620
Epitope Seq.  TAFTIPSI
Variant Seq.  TAFTIPSv
Mutations I/V
Epitope Location I8V
HXB2 Location I290V
Mutation Type DR: diminished response
Epitope Subtype B
Variant Subtype B
Method Chromium-release assay
Note This naturally occurring, strain CAMI variant, was not well recognized by a Patient LWS CTL clone. [Sipsas1997].


Variant ID.  1621
Epitope Seq.  TAFTIPSI
Variant Seq.  TvFTIPSt
Mutations A/V I/T
Epitope Location A2V I8T
HXB2 Location A284V I290T
Mutation Type DR: diminished response
Epitope Subtype B
Variant Subtype B
Method Chromium-release assay
Note This naturally occurring, strain MANC variant, was very poorly recognized by a Patient LWS CTL clone.

References

Menendez-Arias1998 L. Menendez-Arias, A. Mas, and E. Domingo. Cytotoxic T-Lymphocyte Responses to HIV-1 Reverse Transcriptase. Viral Immunol., 11:167-181, 1998. PubMed ID: 10189185. Show all entries for this paper.

Sipsas1997 N. V. Sipsas, S. A. Kalams, A. Trocha, S. He, W. A. Blattner, B. D. Walker, and R. P. Johnson. Identification of Type-Specific Cytotoxic T Lymphocyte Responses to Homologous Viral Proteins in Laboratory Workers Accidentally Infected with HIV-1. J. Clin. Invest., 99:752-762, 1997. To examine a situation where the autologous strain and the reference reagents would be the same, the CTL response of three lab workers accidentally infected with HIV IIIB was studied. Both group specific and type specific epitopes were targets for CTL clones. One subject had a broadening of CTL response over time, using a broad range of restricting HLA class I alleles. Characterization of the cytotoxic T lymphocyte (CTL) response against HIV-1 has been limited by the use of target cells expressing viral proteins from laboratory isolates of HIV-1. This approach has favored identification of group-specific CTL responses and precluded assessment of the extent of type-specific CTL responses directed against HIV-1. Using cells expressing viral proteins from the HIV-1 IIIB strain, we performed a detailed characterization of HIV-1-specific CTL response in three laboratory workers accidentally infected with HIV-1 IIIB. Eight of the epitopes identified were group specific, lying in relatively conserved regions of Gag, reverse transcriptase, and envelope. Three type-specific epitopes were identified, two of them in highly variable regions of envelope. In longitudinal studies in one subject, seven different epitopes and five different restricting HLA class I alleles were identified, with a progressive increase in the number of CTL epitopes recognized by this subject over time. Our data demonstrate that type-specific CTL responses make up a significant proportion of the host cellular immune response against HIV-1 and that a broadening of epitope specificity may occur. PubMed ID: 9045880. Show all entries for this paper.


Displaying record number 461

HXB2 Location  Pol(330-338)   Pol Epitope Map
View variants at this location
Epitope NPDIVIYQY   Epitope Alignment
Variants
hPDIVIYQY   susceptible form
hPDIlIYQY   susceptible form
NPDIiIYQY   susceptible form
NPeIVIYQY   susceptible form
NPDlVIYQY   susceptible form
Species (MHC/HLA human(B35)

Variant Details

Showing all: 5 variant(s).


Variant ID.  1266
Epitope Seq.  NPDIVIYQY
Variant Seq.  hPDIVIYQY
Mutations N/H
Epitope Location N1H
HXB2 Location N330H
Mutation Type SF: susceptible form
Epitope Subtype B
Variant Subtype A
Note This variant is the HIV-1 Clade A consensus and obtained by a Medline database search in August 1998.


Variant ID.  1267
Epitope Seq.  NPDIVIYQY
Variant Seq.  hPDIlIYQY
Mutations N/H V/L
Epitope Location N1H V5L
HXB2 Location N330H V334L
Mutation Type SF: susceptible form
Epitope Subtype B
Note This HIV-2 homologous epitope is recognized by NPDIVIYQY-specific clones.


Variant ID.  1629
Epitope Seq.  NPDIVIYQY
Variant Seq.  NPDIiIYQY
Mutations V/I
Epitope Location V5I
HXB2 Location V334I
Mutation Type SF: susceptible form
Epitope Subtype B
Variant Subtype B
Method Chromium-release assay
Note This naturally occurring, strain JRCSF variant, was recognized by a Patient LWF CTL clone.


Variant ID.  1630
Epitope Seq.  NPDIVIYQY
Variant Seq.  NPeIVIYQY
Mutations D/E
Epitope Location D3E
HXB2 Location D332E
Mutation Type SF: susceptible form
Epitope Subtype B
Variant Subtype B
Method Chromium-release assay
Note This naturally occurring, strain JRU2RF variant, was recognized by a Patient LWF CTL clone.


Variant ID.  1631
Epitope Seq.  NPDIVIYQY
Variant Seq.  NPDlVIYQY
Mutations I/L
Epitope Location I4L
HXB2 Location I333L
Mutation Type SF: susceptible form
Epitope Subtype B
Variant Subtype B
Method Chromium-release assay
Note This naturally occurring variant was recognized by a Patient LWF CTL clone.

References

Menendez-Arias1998 L. Menendez-Arias, A. Mas, and E. Domingo. Cytotoxic T-Lymphocyte Responses to HIV-1 Reverse Transcriptase. Viral Immunol., 11:167-181, 1998. PubMed ID: 10189185. Show all entries for this paper.

Sipsas1997 N. V. Sipsas, S. A. Kalams, A. Trocha, S. He, W. A. Blattner, B. D. Walker, and R. P. Johnson. Identification of Type-Specific Cytotoxic T Lymphocyte Responses to Homologous Viral Proteins in Laboratory Workers Accidentally Infected with HIV-1. J. Clin. Invest., 99:752-762, 1997. To examine a situation where the autologous strain and the reference reagents would be the same, the CTL response of three lab workers accidentally infected with HIV IIIB was studied. Both group specific and type specific epitopes were targets for CTL clones. One subject had a broadening of CTL response over time, using a broad range of restricting HLA class I alleles. Characterization of the cytotoxic T lymphocyte (CTL) response against HIV-1 has been limited by the use of target cells expressing viral proteins from laboratory isolates of HIV-1. This approach has favored identification of group-specific CTL responses and precluded assessment of the extent of type-specific CTL responses directed against HIV-1. Using cells expressing viral proteins from the HIV-1 IIIB strain, we performed a detailed characterization of HIV-1-specific CTL response in three laboratory workers accidentally infected with HIV-1 IIIB. Eight of the epitopes identified were group specific, lying in relatively conserved regions of Gag, reverse transcriptase, and envelope. Three type-specific epitopes were identified, two of them in highly variable regions of envelope. In longitudinal studies in one subject, seven different epitopes and five different restricting HLA class I alleles were identified, with a progressive increase in the number of CTL epitopes recognized by this subject over time. Our data demonstrate that type-specific CTL responses make up a significant proportion of the host cellular immune response against HIV-1 and that a broadening of epitope specificity may occur. PubMed ID: 9045880. Show all entries for this paper.


Displaying record number 620

HXB2 Location  gp160(2-10)   gp160 Epitope Map
View variants at this location
Epitope RVKEKYQHL   Epitope Alignment
Variants
RVKgirknYQHL   non-susceptible form
Species (MHC/HLA human(B8)

Variant Details

Showing all: 1 variant(s).


Variant ID.  1617
Epitope Seq.  RVKEKYQHL
Variant Seq.  RVKgirknYQHL
Mutations E/G K/I Y/R Q/K H/N L/Y
Epitope Location E4G K5I Y6R Q7K H8N L9Y
HXB2 Location E5G K6I Y7R Q8K H9N L10Y
Mutation Type NSF: non-susceptible form
Epitope Subtype B
Variant Subtype B
Method Chromium-release assay
Note This naturally occurring, strain JRCSF variant, was not recognized by a Patient LWS CTL clone.

References

Sipsas1997 N. V. Sipsas, S. A. Kalams, A. Trocha, S. He, W. A. Blattner, B. D. Walker, and R. P. Johnson. Identification of Type-Specific Cytotoxic T Lymphocyte Responses to Homologous Viral Proteins in Laboratory Workers Accidentally Infected with HIV-1. J. Clin. Invest., 99:752-762, 1997. To examine a situation where the autologous strain and the reference reagents would be the same, the CTL response of three lab workers accidentally infected with HIV IIIB was studied. Both group specific and type specific epitopes were targets for CTL clones. One subject had a broadening of CTL response over time, using a broad range of restricting HLA class I alleles. Characterization of the cytotoxic T lymphocyte (CTL) response against HIV-1 has been limited by the use of target cells expressing viral proteins from laboratory isolates of HIV-1. This approach has favored identification of group-specific CTL responses and precluded assessment of the extent of type-specific CTL responses directed against HIV-1. Using cells expressing viral proteins from the HIV-1 IIIB strain, we performed a detailed characterization of HIV-1-specific CTL response in three laboratory workers accidentally infected with HIV-1 IIIB. Eight of the epitopes identified were group specific, lying in relatively conserved regions of Gag, reverse transcriptase, and envelope. Three type-specific epitopes were identified, two of them in highly variable regions of envelope. In longitudinal studies in one subject, seven different epitopes and five different restricting HLA class I alleles were identified, with a progressive increase in the number of CTL epitopes recognized by this subject over time. Our data demonstrate that type-specific CTL responses make up a significant proportion of the host cellular immune response against HIV-1 and that a broadening of epitope specificity may occur. PubMed ID: 9045880. Show all entries for this paper.


Displaying record number 672

HXB2 Location  gp160(156-165)   gp160 Epitope Map
View variants at this location
Epitope NCSFNISTSI   Epitope Alignment
Variants
NCSFNItTSI   non-susceptible form
Species (MHC/HLA human(Cw8)

Variant Details

Showing all: 1 variant(s).


Variant ID.  1618
Epitope Seq.  NCSFNISTSI
Variant Seq.  NCSFNItTSI
Mutations S/T
Epitope Location S7T
HXB2 Location S162T
Mutation Type NSF: non-susceptible form
Epitope Subtype B
Variant Subtype B
Method Chromium-release assay
Note This naturally occurring, strain MN variant, was not recognized by a Patient LWF CTL clone.

References

Sipsas1997 N. V. Sipsas, S. A. Kalams, A. Trocha, S. He, W. A. Blattner, B. D. Walker, and R. P. Johnson. Identification of Type-Specific Cytotoxic T Lymphocyte Responses to Homologous Viral Proteins in Laboratory Workers Accidentally Infected with HIV-1. J. Clin. Invest., 99:752-762, 1997. To examine a situation where the autologous strain and the reference reagents would be the same, the CTL response of three lab workers accidentally infected with HIV IIIB was studied. Both group specific and type specific epitopes were targets for CTL clones. One subject had a broadening of CTL response over time, using a broad range of restricting HLA class I alleles. Characterization of the cytotoxic T lymphocyte (CTL) response against HIV-1 has been limited by the use of target cells expressing viral proteins from laboratory isolates of HIV-1. This approach has favored identification of group-specific CTL responses and precluded assessment of the extent of type-specific CTL responses directed against HIV-1. Using cells expressing viral proteins from the HIV-1 IIIB strain, we performed a detailed characterization of HIV-1-specific CTL response in three laboratory workers accidentally infected with HIV-1 IIIB. Eight of the epitopes identified were group specific, lying in relatively conserved regions of Gag, reverse transcriptase, and envelope. Three type-specific epitopes were identified, two of them in highly variable regions of envelope. In longitudinal studies in one subject, seven different epitopes and five different restricting HLA class I alleles were identified, with a progressive increase in the number of CTL epitopes recognized by this subject over time. Our data demonstrate that type-specific CTL responses make up a significant proportion of the host cellular immune response against HIV-1 and that a broadening of epitope specificity may occur. PubMed ID: 9045880. Show all entries for this paper.


Displaying record number 806

HXB2 Location  gp160(557-565)   gp160 Epitope Map
View variants at this location
Epitope RAIEAQQHL   Epitope Alignment
Variants
kAIEAQQHL   susceptible form
RAIEAQQHm   susceptible form
RAIdAQQHL   susceptible form
RAIkAQQHL   susceptible form
Species (MHC/HLA human(B51)

Variant Details

Showing all: 4 variant(s).


Variant ID.  1632
Epitope Seq.  RAIEAQQHL
Variant Seq.  kAIEAQQHL
Mutations R/K
Epitope Location R1K
HXB2 Location R557K
Mutation Type SF: susceptible form
Epitope Subtype B
Variant Subtype B
Method Chromium-release assay
Note This naturally occurring, strain NY5CG variant, was recognized by a Patient LWS CTL clone.


Variant ID.  1633
Epitope Seq.  RAIEAQQHL
Variant Seq.  RAIEAQQHm
Mutations L/M
Epitope Location L9M
HXB2 Location L565M
Mutation Type SF: susceptible form
Epitope Subtype B
Variant Subtype B
Method Chromium-release assay
Note This naturally occurring, strain JRCSF variant, was recognized by a Patient LWS CTL clone.


Variant ID.  1634
Epitope Seq.  RAIEAQQHL
Variant Seq.  RAIdAQQHL
Mutations E/D
Epitope Location E4D
HXB2 Location E560D
Mutation Type SF: susceptible form
Epitope Subtype B
Variant Subtype B
Method Chromium-release assay
Note This naturally occurring, strain ETR variant, was recognized by a Patient LWS CTL clone.


Variant ID.  1635
Epitope Seq.  RAIEAQQHL
Variant Seq.  RAIkAQQHL
Mutations E/K
Epitope Location E4K
HXB2 Location E560K
Mutation Type SF: susceptible form
Epitope Subtype B
Variant Subtype B
Method Chromium-release assay
Note This naturally occurring, strain CDC42 variant, was recognized by a Patient LWS CTL clone.

References

Sipsas1997 N. V. Sipsas, S. A. Kalams, A. Trocha, S. He, W. A. Blattner, B. D. Walker, and R. P. Johnson. Identification of Type-Specific Cytotoxic T Lymphocyte Responses to Homologous Viral Proteins in Laboratory Workers Accidentally Infected with HIV-1. J. Clin. Invest., 99:752-762, 1997. To examine a situation where the autologous strain and the reference reagents would be the same, the CTL response of three lab workers accidentally infected with HIV IIIB was studied. Both group specific and type specific epitopes were targets for CTL clones. One subject had a broadening of CTL response over time, using a broad range of restricting HLA class I alleles. Characterization of the cytotoxic T lymphocyte (CTL) response against HIV-1 has been limited by the use of target cells expressing viral proteins from laboratory isolates of HIV-1. This approach has favored identification of group-specific CTL responses and precluded assessment of the extent of type-specific CTL responses directed against HIV-1. Using cells expressing viral proteins from the HIV-1 IIIB strain, we performed a detailed characterization of HIV-1-specific CTL response in three laboratory workers accidentally infected with HIV-1 IIIB. Eight of the epitopes identified were group specific, lying in relatively conserved regions of Gag, reverse transcriptase, and envelope. Three type-specific epitopes were identified, two of them in highly variable regions of envelope. In longitudinal studies in one subject, seven different epitopes and five different restricting HLA class I alleles were identified, with a progressive increase in the number of CTL epitopes recognized by this subject over time. Our data demonstrate that type-specific CTL responses make up a significant proportion of the host cellular immune response against HIV-1 and that a broadening of epitope specificity may occur. PubMed ID: 9045880. Show all entries for this paper.


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