Search the Epitope Variant and Escape Mutation Database

Help

Found 2 matching records:

Displaying record number 40

HXB2 Location Gag(24-31) Gag Epitope Map
View variants at this location

GGKKKYKL

`GGKKqYKL`		escape documented in this paper
`erKKqYKL`		observed variant

Species (MHC/HLA) human(B8)

Variant Details

Showing all: 2 variant(s).

Variant ID.	`1440`
Epitope Seq.	`GGKKKYKL`
Variant Seq.	`GGKKqYKL`
Mutations	K/Q
Epitope Location	K5Q
HXB2 Location	K28Q
Mutation Type	E: escape documented in this paper
Epitope Subtype	B
Variant Subtype	B
Method	Chromium-release assay, HLA binding
Note	This variant from the index was seen from the earliest recorded time point. It is either an escape that became fixed or infection occurred with the virus bearing variant Q5. Diminished recognition by index epitope-specific CTL was measured.

Variant ID.	`1441`
Epitope Seq.	`GGKKKYKL`
Variant Seq.	`erKKqYKL`
Mutations	G/E G/R K/Q
Epitope Location	G1E G2R K5Q
HXB2 Location	G24E G25R K28Q
Mutation Type	OV: observed variant
Epitope Subtype	B
Variant Subtype	B
Note	This variant from the index epitope was seen at the earliest recorded time point but was later lost.

References

Price1997 D. A. Price, P. J. Goulder, P. Klenerman, A. K. Sewell, P. J. Easterbrook, M. Troop, C. R. Bangham, and R. E. Phillips. Positive Selection of HIV-1 Cytotoxic T Lymphocyte Escape Variants during Primary Infection. Proc. Natl. Acad. Sci. U.S.A., 94:1890-1895, 1997. Cytotoxic T lymphocytes (CTLs) are thought to play a crucial role in the termination of the acute primary HIV-1 syndrome, but clear evidence for this presumption has been lacking. Here we demonstrate positive selection of HIV-1 proviral sequences encoding variants within a CTL epitope in Nef, a gene product critical for viral pathogenicity, during and after seroconversion. These positively selected HIV-1 variants carried epitope sequence changes that either diminished or escaped CTL recognition. Other proviruses had mutations that abolished the Nef epitope altogether. These results provide clear evidence that CTLs exert selection pressure on the viral population in acute HIV-1 infection. PubMed ID: 9050875. Show all entries for this paper.

Displaying record number 1015

HXB2 Location Nef(90-97) Nef Epitope Map
View variants at this location

Epitope

FLKEKGGL

Epitope Alignment

Variants

`FLKEqGGL`		diminished HLA binding or increased off-rate; escape documented in this paper
`FLKEeGGL`		diminished HLA binding or increased off-rate; escape documented in this paper
`FLKEnGGL`		diminished HLA binding or increased off-rate; escape documented in this paper
`FLKdKGGL`		diminished HLA binding or increased off-rate; escape documented in this paper
`sLKEKGGL`		susceptible form
`lLKEKGGL`		susceptible form
`FiKEnGGL`		diminished HLA binding or increased off-rate; escape documented in this paper
`FLeEnGGL`		diminished HLA binding or increased off-rate; escape documented in this paper
`FLKgnGGL`		diminished HLA binding or increased off-rate; escape documented in this paper
`--------`		epitope loss

Species (MHC/HLA) human(B*08)

Variant Details

Showing all: 10 variant(s).

Variant ID.	`1431`
Epitope Seq.	`FLKEKGGL`
Variant Seq.	`FLKEqGGL`
Mutations	K/Q
Epitope Location	K5Q
HXB2 Location	K94Q
Mutation Type	DHB: diminished HLA binding or increased off-rate E: escape documented in this paper
Method	Chromium-release assay, HLA binding
Note	Reduced binding and recognition

Variant ID.	`1432`
Epitope Seq.	`FLKEKGGL`
Variant Seq.	`FLKEeGGL`
Mutations	K/E
Epitope Location	K5E
HXB2 Location	K94E
Mutation Type	DHB: diminished HLA binding or increased off-rate E: escape documented in this paper
Method	Chromium-release assay, HLA binding
Note	Reduced binding, recognition

Variant ID.	`1433`
Epitope Seq.	`FLKEKGGL`
Variant Seq.	`FLKEnGGL`
Mutations	K/N
Epitope Location	K5N
HXB2 Location	K94N
Mutation Type	DHB: diminished HLA binding or increased off-rate E: escape documented in this paper
Method	Chromium-release assay, HLA binding
Note	Reduced binding, recognition

Variant ID.	`1434`
Epitope Seq.	`FLKEKGGL`
Variant Seq.	`FLKdKGGL`
Mutations	E/D
Epitope Location	E4D
HXB2 Location	E93D
Mutation Type	DHB: diminished HLA binding or increased off-rate E: escape documented in this paper
Method	Chromium-release assay, HLA binding
Note	Reduced binding, recognition

Variant ID.	`1435`
Epitope Seq.	`FLKEKGGL`
Variant Seq.	`sLKEKGGL`
Mutations	F/S
Epitope Location	F1S
HXB2 Location	F90S
Mutation Type	SF: susceptible form
Method	HLA binding
Note	Variant recognized robustly

Variant ID.	`1436`
Epitope Seq.	`FLKEKGGL`
Variant Seq.	`lLKEKGGL`
Mutations	F/L
Epitope Location	F1L
HXB2 Location	F90L
Mutation Type	SF: susceptible form
Method	HLA binding
Note	Variant recognized robustly

Variant ID.	`1437`
Epitope Seq.	`FLKEKGGL`
Variant Seq.	`FiKEnGGL`
Mutations	L/I K/N
Epitope Location	L2I K5N
HXB2 Location	L91I K94N
Mutation Type	DHB: diminished HLA binding or increased off-rate E: escape documented in this paper
Method	HLA binding
Note	Escaped recognition completely

Variant ID.	`1438`
Epitope Seq.	`FLKEKGGL`
Variant Seq.	`FLeEnGGL`
Mutations	K/E K/N
Epitope Location	K3E K5N
HXB2 Location	K92E K94N
Mutation Type	DHB: diminished HLA binding or increased off-rate E: escape documented in this paper
Method	HLA binding
Note	Escaped recognition completely

Variant ID.	`1439`
Epitope Seq.	`FLKEKGGL`
Variant Seq.	`FLKgnGGL`
Mutations	E/G K/N
Epitope Location	E4G K5N
HXB2 Location	E93G K94N
Mutation Type	DHB: diminished HLA binding or increased off-rate E: escape documented in this paper
Method	HLA binding
Note	Escaped recognition completely

Variant ID.	`2655`
Epitope Seq.	`FLKEKGGL`
Variant Seq.	`--------`
Mutations	F/- L/- K/- E/- K/- G/- G/- L/-
Epitope Location	F1- L2- K3- E4- K5- G6- G7- L8-
HXB2 Location	F90- L91- K92- E93- K94- G95- G96- L97-
Mutation Type	EL: epitope loss
Epitope Subtype	B
Variant Subtype	B
Method	Sequence
Note	A single nucleotide deletion within DNA resulted in a premature termination of Nef protein 6 amino acids before the epitope FL8. 2 clones with this truncation of protein and epitope loss were found at separate time points.

References

Goulder1997e P. Goulder, D. Price, M. Nowak, S. Rowland-Jones, R. Phillips, and A. McMichael. Co-Evolution of Human Immunodeficiency Virus and Cytotoxic T-Lymphocyte Responses. Immunol. Rev., 159:17-29, 1997. PubMed ID: 9416500. Show all entries for this paper.