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Found 2 matching records:

Displaying record number 40

HXB2 Location  Gag(24-31)   Gag Epitope Map
View variants at this location
Epitope GGKKKYKL   Epitope Alignment
Variants
GGKKqYKL   escape documented in this paper
erKKqYKL   observed variant
Species (MHC/HLA human(B8)

Variant Details

Showing all: 2 variant(s).


Variant ID.  1440
Epitope Seq.  GGKKKYKL
Variant Seq.  GGKKqYKL
Mutations K/Q
Epitope Location K5Q
HXB2 Location K28Q
Mutation Type E: escape documented in this paper
Epitope Subtype B
Variant Subtype B
Method Chromium-release assay, HLA binding
Note This variant from the index was seen from the earliest recorded time point. It is either an escape that became fixed or infection occurred with the virus bearing variant Q5. Diminished recognition by index epitope-specific CTL was measured.


Variant ID.  1441
Epitope Seq.  GGKKKYKL
Variant Seq.  erKKqYKL
Mutations G/E G/R K/Q
Epitope Location G1E G2R K5Q
HXB2 Location G24E G25R K28Q
Mutation Type OV: observed variant
Epitope Subtype B
Variant Subtype B
Note This variant from the index epitope was seen at the earliest recorded time point but was later lost.

References

Price1997 D. A. Price, P. J. Goulder, P. Klenerman, A. K. Sewell, P. J. Easterbrook, M. Troop, C. R. Bangham, and R. E. Phillips. Positive Selection of HIV-1 Cytotoxic T Lymphocyte Escape Variants during Primary Infection. Proc. Natl. Acad. Sci. U.S.A., 94:1890-1895, 1997. Cytotoxic T lymphocytes (CTLs) are thought to play a crucial role in the termination of the acute primary HIV-1 syndrome, but clear evidence for this presumption has been lacking. Here we demonstrate positive selection of HIV-1 proviral sequences encoding variants within a CTL epitope in Nef, a gene product critical for viral pathogenicity, during and after seroconversion. These positively selected HIV-1 variants carried epitope sequence changes that either diminished or escaped CTL recognition. Other proviruses had mutations that abolished the Nef epitope altogether. These results provide clear evidence that CTLs exert selection pressure on the viral population in acute HIV-1 infection. PubMed ID: 9050875. Show all entries for this paper.


Displaying record number 1015

HXB2 Location  Nef(90-97)   Nef Epitope Map
View variants at this location
Epitope FLKEKGGL   Epitope Alignment
Variants
FLKEqGGL   diminished HLA binding or increased off-rate; escape documented in this paper
FLKEeGGL   diminished HLA binding or increased off-rate; escape documented in this paper
FLKEnGGL   diminished HLA binding or increased off-rate; escape documented in this paper
FLKdKGGL   diminished HLA binding or increased off-rate; escape documented in this paper
sLKEKGGL   susceptible form
lLKEKGGL   susceptible form
FiKEnGGL   diminished HLA binding or increased off-rate; escape documented in this paper
FLeEnGGL   diminished HLA binding or increased off-rate; escape documented in this paper
FLKgnGGL   diminished HLA binding or increased off-rate; escape documented in this paper
--------   epitope loss
Species (MHC/HLA human(B*08)

Variant Details

Showing all: 10 variant(s).


Variant ID.  1431
Epitope Seq.  FLKEKGGL
Variant Seq.  FLKEqGGL
Mutations K/Q
Epitope Location K5Q
HXB2 Location K94Q
Mutation Type DHB: diminished HLA binding or increased off-rate
E: escape documented in this paper
Method Chromium-release assay, HLA binding
Note Reduced binding and recognition


Variant ID.  1432
Epitope Seq.  FLKEKGGL
Variant Seq.  FLKEeGGL
Mutations K/E
Epitope Location K5E
HXB2 Location K94E
Mutation Type DHB: diminished HLA binding or increased off-rate
E: escape documented in this paper
Method Chromium-release assay, HLA binding
Note Reduced binding, recognition


Variant ID.  1433
Epitope Seq.  FLKEKGGL
Variant Seq.  FLKEnGGL
Mutations K/N
Epitope Location K5N
HXB2 Location K94N
Mutation Type DHB: diminished HLA binding or increased off-rate
E: escape documented in this paper
Method Chromium-release assay, HLA binding
Note Reduced binding, recognition


Variant ID.  1434
Epitope Seq.  FLKEKGGL
Variant Seq.  FLKdKGGL
Mutations E/D
Epitope Location E4D
HXB2 Location E93D
Mutation Type DHB: diminished HLA binding or increased off-rate
E: escape documented in this paper
Method Chromium-release assay, HLA binding
Note Reduced binding, recognition


Variant ID.  1435
Epitope Seq.  FLKEKGGL
Variant Seq.  sLKEKGGL
Mutations F/S
Epitope Location F1S
HXB2 Location F90S
Mutation Type SF: susceptible form
Method HLA binding
Note Variant recognized robustly


Variant ID.  1436
Epitope Seq.  FLKEKGGL
Variant Seq.  lLKEKGGL
Mutations F/L
Epitope Location F1L
HXB2 Location F90L
Mutation Type SF: susceptible form
Method HLA binding
Note Variant recognized robustly


Variant ID.  1437
Epitope Seq.  FLKEKGGL
Variant Seq.  FiKEnGGL
Mutations L/I K/N
Epitope Location L2I K5N
HXB2 Location L91I K94N
Mutation Type DHB: diminished HLA binding or increased off-rate
E: escape documented in this paper
Method HLA binding
Note Escaped recognition completely


Variant ID.  1438
Epitope Seq.  FLKEKGGL
Variant Seq.  FLeEnGGL
Mutations K/E K/N
Epitope Location K3E K5N
HXB2 Location K92E K94N
Mutation Type DHB: diminished HLA binding or increased off-rate
E: escape documented in this paper
Method HLA binding
Note Escaped recognition completely


Variant ID.  1439
Epitope Seq.  FLKEKGGL
Variant Seq.  FLKgnGGL
Mutations E/G K/N
Epitope Location E4G K5N
HXB2 Location E93G K94N
Mutation Type DHB: diminished HLA binding or increased off-rate
E: escape documented in this paper
Method HLA binding
Note Escaped recognition completely


Variant ID.  2655
Epitope Seq.  FLKEKGGL
Variant Seq.  --------
Mutations F/- L/- K/- E/- K/- G/- G/- L/-
Epitope Location F1- L2- K3- E4- K5- G6- G7- L8-
HXB2 Location F90- L91- K92- E93- K94- G95- G96- L97-
Mutation Type EL: epitope loss
Epitope Subtype B
Variant Subtype B
Method Sequence
Note A single nucleotide deletion within DNA resulted in a premature termination of Nef protein 6 amino acids before the epitope FL8. 2 clones with this truncation of protein and epitope loss were found at separate time points.

References

Goulder1997e P. Goulder, D. Price, M. Nowak, S. Rowland-Jones, R. Phillips, and A. McMichael. Co-Evolution of Human Immunodeficiency Virus and Cytotoxic T-Lymphocyte Responses. Immunol. Rev., 159:17-29, 1997. PubMed ID: 9416500. Show all entries for this paper.

Price1997 D. A. Price, P. J. Goulder, P. Klenerman, A. K. Sewell, P. J. Easterbrook, M. Troop, C. R. Bangham, and R. E. Phillips. Positive Selection of HIV-1 Cytotoxic T Lymphocyte Escape Variants during Primary Infection. Proc. Natl. Acad. Sci. U.S.A., 94:1890-1895, 1997. Cytotoxic T lymphocytes (CTLs) are thought to play a crucial role in the termination of the acute primary HIV-1 syndrome, but clear evidence for this presumption has been lacking. Here we demonstrate positive selection of HIV-1 proviral sequences encoding variants within a CTL epitope in Nef, a gene product critical for viral pathogenicity, during and after seroconversion. These positively selected HIV-1 variants carried epitope sequence changes that either diminished or escaped CTL recognition. Other proviruses had mutations that abolished the Nef epitope altogether. These results provide clear evidence that CTLs exert selection pressure on the viral population in acute HIV-1 infection. PubMed ID: 9050875. Show all entries for this paper.


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