Search T Helper/CD4+ T-Cell Epitope Database

Found 2 matching records:

Displaying record number 201409

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HXB2 Location	Gag(265-282) p24(133-150) DNA(1582..1635)	Gag Epitope Map
Author Location	p24
Epitope	WIILGLNKIVRMYSPVSI	Epitope Alignment
Subtype	C
Species (MHC/HLA)	human
Immunogen	HIV-1 infection
Country	South Africa
Experimental methods	CD4 T-cell Elispot - IFNγ
Keywords	immunodominance

Notes

• CD4+ T cell responses were measured in a cohort of C-clade infected chronic, untreated subjects from Durban South Africa. Intracellular cytokine staining detected IFN-γ responses in 261/373 subjects, with the Gag responders having a significantly lower viral load and higher CD4 count than those with no detectable Gag response. Helper epitopes were determined by separate EliSpot screening on 32 subjects, using peptides spanning the entire clade C consensus sequence.
• 33 CD4+ peptides were targeted by 18/32 patients, with 27/33 peptides located in Gag region. 15/18 subjects targeted Gag, indicating a relative immunodominance of Gag. The magnitude of response did not correlate with viral load, but breadth of response had strong inverse correlation with viral load.
• WIILGLNKIVRMYSPVSI was targeted by 2/32 C-clade subjects.

References

Ramduth2009 Danni Ramduth, Cheryl L. Day, Christina F. Thobakgale, Nompumelelo P. Mkhwanazi, Chantal de Pierres, Sharon Reddy, Mary van der Stok, Zenele Mncube, Kriebashne Nair, Eshia S. Moodley, Daniel E. Kaufmann, Hendrik Streeck, Hoosen M. Coovadia, Photini Kiepiela, Philip J. R. Goulder, and Bruce D. Walker. Immunodominant HIV-1 CD4+ T Cell Epitopes in Chronic Untreated Clade C HIV-1 Infection. PLoS One, 4(4):e5013, 2009. PubMed ID: 19352428. Show all entries for this paper.

Displaying record number 201438

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HXB2 Location	Gag(265-282) p24(133-150) DNA(1582..1635)	Gag Epitope Map
Author Location	p24(265-282)
Epitope	WIILGLNKIVRMYSPVSI	Epitope Alignment
Subtype	C
Species (MHC/HLA)	baboon
Immunogen	vaccine
Country	South Africa
Experimental methods	Intracellular cytokine staining, T-cell Elispot
Keywords	vaccine-induced immune responses

Vaccine Details

Notes

• Immune responses in chacma baboons to candidate DNA and MVA vaccines are described. The vaccines express 5 genes (gag, RT, tat, nef, env) from HIV clade C.
• The vaccine induced strong and broad T-cell responses targeting all 5 genes, with a median of 12 targeted peptides per animal and without any single protein dominating the response. The vaccine induced strong IFN-γ and IL-2 responses with a balanced distribution in the CD8+ and CD4+ T-cell compartments. Mucosal immunity as well as long-lasting responses, present over a year after vaccination were induced.
• Responses were mapped with IFN-γ ELISPOT assay and where cells were available, the phenotype of the response was determined using intracellular cytokine staining.
• This peptide was targeted by one animal in ELISPOT and phenotyped as CD4 response. It contains previously determined CD4 epitope NKIVRMYSPTSI (DRB1*1101, DRB1*1501,DRB5*0101.

References

Burgers2009 Wendy A. Burgers, Gerald K. Chege, Tracey L. Muller, Joanne H. van Harmelen, Greg Khoury, Enid G. Shephard, Clive M. Gray, Carolyn Williamson, and Anna-Lise Williamson. Broad, High-Magnitude and Multifunctional CD4+ and CD8+ T-Cell Responses Elicited by a DNA and Modified Vaccinia Ankara Vaccine Containing Human Immunodeficiency Virus Type 1 Subtype C Genes in Baboons. J. Gen. Virol., 90(Pt 2):468-480, Feb 2009. PubMed ID: 19141458. Show all entries for this paper.