Search T Helper/CD4+ T-Cell Epitope Database

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Displaying record number 491

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HXB2 Location	gp160(606-620) gp41(95-109) DNA(8040..8084)	gp160 Epitope Map
Author Location	gp41( UG92005)
Epitope	`TNVPWNASWSNKSLE`	Epitope Alignment
Species (MHC/HLA)	mouse(H-2 IA^b)
Immunogen	vaccine
Experimental methods
Keywords	subtype comparisons, epitope processing, TCR usage

Vaccine Details

Vaccine type	DNA, protein, vaccinia
Vaccine strain	B clade 1007, D clade UG92005
Vaccine component	gp140
Adjuvant	Complete Freund's Adjuvant (CFA)

Notes

This gp140 epitope of UG92005 (UG, clade D) was recognized by five hybridomas with Vβ usage Vβ 8.1, 14 and not determined -- one of the Vβ 8.1 was shown to utilize Vα 8.
C57BL/6 mice were immunized with a prime-boost strategy involving three HIV-1 Env antigens: Mice were primed with DNA given i.m., 3-4 weeks later boosted with VV, and 3-4 weeks later boosted again with purified protein in Freund's adjuvant.
The vaccinia construct is a pSC11-based VV vector with the first 38 amino acids contributed by BH10 and the rest of gp120 and gp41 by the vaccine strain, the DNA construct is in the pJW4303 vector with a CMV promotor, and the purified protein is expressed from the pJW4303 vector transfected into CHO-K1 cells.
Ten days after the final boost, hybridomas were made and tested for IL-2 production using either B6 spleen cells or H-2 IA^b transfected L cells as targets and Vβ usage was determined.
Mice were immunized with an Env from either one of two clades: HIV-1 1007, a clade B strain isolated from an individual from Memphis Tennesee, and HIV-1 92UG005, a clade D vaccine isolated from Uganda in 1992 through the WHO.
80 unique clonotypes were characterized from six mice.
H-2 IA^b restricted T-helper epitopes were concentrated in 5 distinct regions within the Env sequence (2 clonotype responses in V2, 26 in C2, 22 in V3, 23 in V4C4, and 7 in gp41).
Epitope hotspots tended to be proximal to heavily glycosylated regions of the Env sequence, in exposed, non-helical strands of the protein. The non-uniform localization may be influenced by differential antigen processing and the glycosylation site proximity may allow binding to lectins and promote trafficking through processing pathways.

References

Surman2001 S. Surman, T. D. Lockey, K. S. Slobod, B. Jones, J. M. Riberdy, S. W. White, P. C. Doherty, and J. L. Hurwitz. Localization of CD4+ T Cell Epitope Hotspots to Exposed Strands of HIV Envelope Glycoprotein Suggests Structural Influences on Antigen Processing. Proc. Natl. Acad. Sci. U.S.A., 98(8):4587-4592, 10 Apr 2001. URL: http://www.pnas.org/cgi/content/full/98/8/4587. PubMed ID: 11287644. Show all entries for this paper.

Displaying record number 200877

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HXB2 Location	gp160(606-620) gp41(95-109) DNA(8040..8084)	gp160 Epitope Map
Author Location	gp41( 1035)
Epitope	`TNVPWNASWSNKSLE`	Epitope Alignment
Subtype	B
Species (MHC/HLA)	mouse
Immunogen	vaccine
Experimental methods	T-cell Elispot
Keywords	epitope processing, vaccine-induced immune responses, escape, TCR usage

Vaccine Details

Vaccine type	vaccinia prime with gp120 boost
Vaccine strain	B clade 1035
Vaccine component	Env
Adjuvant	Complete Freund's Adjuvant (CFA)

Notes

A very narrow Th response was stimulated in C57BL/6 mice vaccinated with vaccinia expressed HIV-1 env clone 1035, to the peptide PKVSFEPIPIHYCAP, located in the C2 region of gp120. The only other peptide recognized using Elispot on Env overlapping peptides to test vaccine responses in the mice was this one: TNVPWNASWSNKSLE, located in gp41.

References

Zhan2003 X. Zhan, K. S. Slobod, S. Surman, S. A. Brown, T. D. Lockey, C. Coleclough, P. C. Doherty, and J. L. Hurwitz. Limited Breadth of a T-Helper Cell Response to a Human Immunodeficiency Virus Envelope Protein. J. Virol., 77(7):4231-4236, Apr 2003. PubMed ID: 12634380. Show all entries for this paper.