Search T Helper/CD4+ T-Cell Epitope Database

Found 5 matching records:

Displaying record number 201525

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HXB2 Location	Gag(257-271) p24(125-139) DNA(1558..1602)	Gag Epitope Map
Author Location	Gag(257-271 HXB2)
Epitope	`PVGEIYKRWIILGLN`	Epitope Alignment
Subtype	B
Species (MHC/HLA)	transgenic mouse(DRB1*01:01)
Immunogen	vaccine
Experimental methods	T-cell Elispot
Keywords	vaccine-induced immune responses, dendritic cells

Vaccine Details

Vaccine type	DNA prime with peptide boost, DNA
Vaccine strain	B clade HXB2
Vaccine component	Gag

Notes

Dendritic cell mediated immunization of DNA plasmid constructs was studied in BALB/c (H-2d) and HLA-DR4 (DRA1*0101, DRB1*0401) transgenic mice. Most peptide-specific T-cell responses in the HLA-DR4 transgenic mice corresponded to human HLA-DR4 epitopes listed in HIV Immunology Database.
Gag peptide PVGEIYKRWIILGLN elicited CD4 T-cell responses in HLA-DR4 (DRB1*0401) transgenic mice immunized with pLAMP/gag-based prime and boost formulations.

References

Simon2010 Gregory G. Simon, Yongli Hu, Asif M. Khan, Jingshi Zhou, Jerome Salmon, Priya R. Chikhlikar, Keun-Ok Jung, Ernesto T. A. Marques, and J. Thomas August. Dendritic Cell Mediated Delivery of Plasmid DNA Encoding LAMP/HIV-1 Gag Fusion Immunogen Enhances T Cell Epitope Responses in HLA DR4 Transgenic Mice. PLoS One, 5(1):e8574, 2010. PubMed ID: 20052293. Show all entries for this paper.

Displaying record number 201526

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HXB2 Location	Gag(257-271) p24(125-139) DNA(1558..1602)	Gag Epitope Map
Author Location	Gag(257-271 HXB2)
Epitope	`PVGEIYKRWIILGLN`	Epitope Alignment
Subtype	B
Species (MHC/HLA)	transgenic mouse(DRB1*01:01)
Immunogen	vaccine
Experimental methods	T-cell Elispot
Keywords	vaccine-induced immune responses, dendritic cells

Vaccine Details

Vaccine type	DNA prime with peptide boost, DNA
Vaccine strain	B clade HXB2
Vaccine component	Gag

Notes

Dendritic cell mediated immunization of DNA plasmid constructs was studied in BALB/c (H-2d) and HLA-DR4 (DRA1*0101, DRB1*0401) transgenic mice. Most peptide-specific T-cell responses in the HLA-DR4 transgenic mice corresponded to human HLA-DR4 epitopes listed in HIV Immunology Database.
Gag peptide PVGEIYKRWIILGLN elicited CD4 T-cell responses in HLA-DR4 (DRB1*0401) transgenic mice immunized with pLAMP/gag-based prime and boost formulations.

References

Displaying record number 201283

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HXB2 Location	Gag(257-271) p24(125-139) DNA(1558..1602)	Gag Epitope Map
Author Location	Gag(257-271 SF2)
Epitope	`PVGEIYKRWIILGLN`	Epitope Alignment
Epitope Name	Peptide 65
Subtype	B
Species (MHC/HLA)	mouse(H-2^d)
Immunogen	vaccine
Experimental methods	CD8 T-cell Elispot - IFNγ, Chromium-release assay
Keywords	vaccine-induced immune responses

Vaccine Details

Vaccine type	protein
Vaccine strain	B clade IIIB
Vaccine component	Gag, Tat
Adjuvant	Complete Freund's Adjuvant (CFA), Incomplete Freund's Adjuvant (IFA)

Notes

In order to increase the known number of epitopes in Gag protein so that they can be assayed for reactivity in vaccine studies, BALB/c mice were inoculated with Gag or Gag+Tat. OLPs were used to elicit T-cell immune responses from mouse splenocytes and tested for by cytotoxicity or ELISpot assays. 5 novel predicted optimal CTL epitopes, 2 novel predicted CD4 epitopes and 2 immune response eliciting peptides were found.
Peptide PVGEIYKRWIILGLN contains a previously defined Gag CD4 epitope and IYKRWIILGL CD8 epitope.

References

Cellini2008 Silvia Cellini, Cinzia Fortini, Eleonora Gallerani, Federica Destro, Egidio Brocca Cofano, Antonella Caputo, and Riccardo Gavioli. Identification of New HIV-1 Gag-Specific Cytotoxic T Lymphocyte Responses in BALB/c Mice. Virol. J., 5:81, 2008. PubMed ID: 18625037. Show all entries for this paper.

Displaying record number 201069

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HXB2 Location	Gag(257-271) p24(125-139) DNA(1558..1602)	Gag Epitope Map
Author Location	Gag(257-271 HXB-2)
Epitope	`PVGEIYKRWIILGLN`	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human
Immunogen	HIV-1 infection
Patient MHC/HLA	DQB103:01, DQB106:01, DRB113:03, DRB115:02, DRB301:01, DRB501:02
Country	United States
Experimental methods	CD4 T-cell Elispot - IFNγ
Keywords

Notes

The study measured CD4+ T-cell responses against epitopes in Gag p17 and p24 and concurrent endogenous plasma HIV-1 RNA epitope sequence variation. No intrapatient protein sequence variation was found in identified epitopes, indicating that escape from CD4-positive T-cell responses is not a common process in vivo.
2/22 patients responded to this peptide.

References

Koeppe2006 John R. Koeppe, Thomas B. Campbell, Eric L. Rapaport, and Cara C. Wilson. HIV-1-Specific CD4+ T-Cell Responses Are Not Associated With Significant Viral Epitope Variation in Persons With Persistent Plasma Viremia. J. Acquir. Immune Defic. Syndr., 41(2):140-148, 1 Feb 2006. PubMed ID: 16394844. Show all entries for this paper.

Displaying record number 201093

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HXB2 Location	Gag(257-271) p24(125-139) DNA(1558..1602)	Gag Epitope Map
Author Location
Epitope	`PVGEIYKRWIILGLN`	Epitope Alignment
Epitope Name	G065
Species (MHC/HLA)	human
Immunogen	HIV-1 infection
Country	Canada
Experimental methods	Flow cytometric T-cell cytokine assay, Proliferation
Keywords	memory cells

Notes

HIV-1-specific CD4+ T-cell responses were analyzed for 6 years since primary infection, in 10 aviremic and 8 viremic patients.
CD4+ T cells proliferating responses were correlated with the frequency of CD4+ T cells secreting IL-2 in aviremic patients. In viremic patients, CD4+ T cell proliferative response was impaired despite of the high frequencies of IFN-γ, but not IL-2-producing CD4+ T cells in periods of elevated viremia, suggesting that long-term CD4+ memory depends on IL-2-producing CD4+ T cells and that IFN-γ only-producing cells are short lived.

References

Younes2003 Souheil-Antoine Younes, Bader Yassine-Diab, Alain R. Dumont, Mohamed-Rachid Boulassel, Zvi Grossman, Jean-Pierre Routy, and Rafick-Pierre Sékaly. HIV-1 Viremia Prevents the Establishment of Interleukin 2-Producing HIV-Specific memory CD4+ T Cells Endowed with Proliferative Capacity. J. Exp. Med., 198(12):1909-1922, 15 Dec 2003. PubMed ID: 14676302. Show all entries for this paper.