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Displaying record number 201997

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HXB2 Location	Nef(136-150) DNA(9202..9246)	Nef Epitope Map
Author Location	Nef
Epitope	`PLTFGWCFKLVPVDP`	Epitope Alignment
Epitope Name	Nef 144
Species (MHC/HLA)	human, mouse
Immunogen	in vitro stimulation or selection, HIV-1 infection, vaccine
Country	China
Experimental methods	CD4 T-cell Elispot - IFNγ, HLA binding, Other
Keywords	binding affinity, vaccine-induced immune responses, kinetics, vaccine antigen design

Vaccine Details

Vaccine type	polyepitope, E. coli recombinant protein
Vaccine component	Env, Gag, Nef, Pol

Notes

6/20 epitopes selected bioinformatically for a recombinant polyepitope CD4+ T cell HIV vaccine, HIV20Th, elicited a response from CD4+ enriched PBMCs in at least 15% of 46 HIV-infected patients but all 20 epitopes elicited a response in at least one patient. 5/20 epitopes elicited a response in HIV20Th-immunized Balb/c mice. While a positive correlation was found between magnitude of the T-cell response and the amount of epitope bound to MHC II in HIV20Th-immunized Balb/c splenocytes, no correlation was found to in vitro peptide digestion patterns, binding affinities, or peptide-MHC dissociation assays. Half of the HIV20Th epitopes were included in a modified recombinant polyepitope CD4+ T cell HIV vaccine, HIV20Th-2, which elicited a response for all 10/10 epitopes in immunized Balb/c mice suggesting that in vivo competition for MHC molecules may limit the breadth of induced T-cell responses.
PLTFGWCFKLVPVDP (Nef 144) stimulated a CD4+ IFNγ EliSpot response in about 7% of 46 HIV-infected patients and HIV20Th-2-immunized Balb/c mice but not in HIV20Th-immunized Balb/c mice.
Nef 144 bound MHC II with high affinity, relative to the 20 peptides included in HIV20Th, and had an off-rate of 0.93 hrs.
A Nef 144-overlapping peptide (GPGPLTFGWCFKLVPVDP) was found presented on MHC II in HIV20Th-immunized immunized Balb/c splenocytes.

References

Shu2020 Jiayi Shu, Wei Shen, Haitao Liu, Yiqing Zhou, Jianhui Li, Yan Zhuang, Zhenghui Huang, Shigang Yin, Lubin Jiang, Yongtao Sun, Xia Jin, and Jianqing Xu. The Immunologic Dominance of an Epitope within a Rationally Designed Poly-Epitope Vaccine Is Influenced by Multiple Factors. Vaccine, 38(14):2913-2924, 23 Mar 2020. PubMed ID: 32127225. Show all entries for this paper.