Search T Helper/CD4+ T-Cell Epitope Database

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Displaying record number 200978

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HXB2 Location	Tat(41-50) DNA(5951..5980)	Tat Epitope Map
Author Location	Tat(40-50 C consensus)
Epitope	KGLGISYGRK?	Epitope Alignment
Subtype	C
Species (MHC/HLA)	mouse
Immunogen	vaccine
Patient MHC/HLA	H-2d
Experimental methods	CD4 T-cell Elispot - IFNγ, Proliferation
Keywords	Th1, vaccine antigen design

Vaccine Details

Notes

• BALB/c and C57BL/6 mice were intramuscularly immunized with a codon optimized HIV-1 C-consensus Tat DNA vaccine that was linked to ubiquitin to facilitate rapid processing. Ubiquitin and codon optimization enhanced Th1 T cell responses, with increased proliferative responses, cytotoxic responses, and Th1 responses measured by IFNgamma EliSpot, but not the Th2 responses, measured by IL-4 EliSpot..
• Several immunogenic regions in HIV-1 Tat were identified in BALB/c mice using EliSpot. The strongest immune response was within the core region of Tat; the peptides based on the C subtype consensus positions 30-50 and 40-60 gave the strongest EliSpot responses in BALB/c mice, suggesting a putative helper T-cell epitope spanning the region of overlap, residues 40-50.

References

Ramakrishna2004 Lakshmi Ramakrishna, Krishnamurthy Kumar Anand, Marthandan Mahalingam, Kumarasamypet M. Mohankumar, Shilpa Ramani, Nagadenahalli B. Siddappa, and Udaykumar Ranga. Codon Optimization and Ubiquitin Conjugation of Human Immunodeficiency Virus-1 Tat Lead to Enhanced Cell-Mediated Immune Responses. Vaccine, 22(20):2586-2598, 30 Jun 2004. PubMed ID: 15193384. Show all entries for this paper.