Search T Helper/CD4+ T-Cell Epitope Database

Found 3 matching records:

Displaying record number 201013

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HXB2 Location	Nef(184-199) DNA(9346..9393)	Nef Epitope Map
Author Location	Nef(184-199 B consensus)
Epitope	KFDSRLAFHHMARELH	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human(DRB1*01:01, DRB1*07:01, DRB1*11:01, DRB1*15:01, DRB5*01:01)
Immunogen	HIV-1 infection
Country	United States
Experimental methods	CD4 T-cell Elispot - IFNγ
Keywords	supervised treatment interruptions (STI), immunodominance

Notes

• CD4 T-cell responses in 36 HIV-1 positive individuals in different disease stages were mapped by IFN-γ EliSpot using a panel of 410 B-consensus peptides spanning all HIV proteins. Virus specific CD4+ T-cell responses (median=7, range of magnitude=990) were detected in 30/36 patients.
• This peptide was recognized by 25% of the study group.
• Gag and Nef responses dominated the CD4+ T-cell EliSpot responses in the study. No correlation between plasma viremia and the frequency or magnitude of HIV-1-specific responses was observed.
• The peptides that were recognized by the most people were able to bind broadly to multiple HLA-DR molecules. This peptide showed high cross-reactive binding capacity and bound to 5/8 tested HLA-DR molecules.
• The most HIV-1 specific CD4+ T-cell responses were observed in acutely infected patients with treatment of primary infection who stopped after 1-3 treatments (acute STI), compared to patients continuing therapy. People with therapy during chronic infection had low responses; higher numbers of responses were seen among untreated people. 2/9 LTNPs responded to many peptides, comparable to acute STI.

References

Kaufmann2004 Daniel E. Kaufmann, Paul M. Bailey, John Sidney, Bradford Wagner, Philip J. Norris, Mary N. Johnston, Lisa A. Cosimi, Marylyn M. Addo, Mathias Lichterfeld, Marcus Altfeld, Nicole Frahm, Christian Brander, Alessandro Sette, Bruce D. Walker, and Eric S. Rosenberg. Comprehensive Analysis of Human Immunodeficiency Virus Type 1-Specific CD4 Responses Reveals Marked Immunodominance of gag and nef and the Presence of Broadly Recognized Peptides. J. Virol., 78(9):4463-4477, May 2004. PubMed ID: 15078927. Show all entries for this paper.

Displaying record number 201748

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HXB2 Location	Nef(184-199) DNA(9346..9393)	Nef Epitope Map
Author Location	Nef
Epitope	KFDSRLAFHHMARELH	Epitope Alignment
Epitope Name	OLP-92
Species (MHC/HLA)	human(DRB1*03:02, DRB1*13:02, DRB1*13:03, DRB3*03:01)
Immunogen	HIV-1 infection
Country	United States
Experimental methods	CD4 T-cell Elispot - IFNγ, HLA binding
Keywords	binding affinity, immunodominance, HLA cross-presentation

Notes

• 67 Gag and Nef peptides restricted by HLA-DRB1 were characterized immunologically in 17 controllers and 9 progressors. HLA allele DRB1*15:02 was associated with low HIV-1 viremia and could promiscuously present peptides with lower avidity. HLA DRB1*03:01, however, was associated with high viremia and specific presentation of peptides. Moreover, Nef peptides were exclusively restricted by HLA-DRB1 variants linked with low viremia. Individuals with low viremia-linked HLA-DRB1 alleles also had greater breadth of HIV-specific CD4+ T cell responses. DRB1 allelic expression can impact HIV replication.
• Overlapping peptide-92 (OLP-92), KFDSRLAFHHMARELH, was promiscuous as it was restricted by 4 different HLA-DR across the HIV proteome as determined by IFN-γ EliSpot.

References

Ranasinghe2013 Srinika Ranasinghe, Sam Cutler, Isaiah Davis, Richard Lu, Damien Z. Soghoian, Ying Qi, John Sidney, Gregory Kranias, Michael D. Flanders, Madelene Lindqvist, Bjorn Kuhl, Galit Alter, Steven G. Deeks, Bruce D. Walker, Xiaojiang Gao, Alessandro Sette, Mary Carrington, and Hendrik Streeck. Association of HLA-DRB1-Restricted CD4+ T Cell Responses with HIV Immune Control. Nat. Med., 19(7):930-933, Jul 2013. PubMed ID: 23793098. Show all entries for this paper.

Displaying record number 201086

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HXB2 Location	Nef(184-199) DNA(9346..9393)	Nef Epitope Map
Author Location	Nef(184-199)
Epitope	KFDSRLAFHHMARELH	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human
Immunogen	HIV-1 infection
Country	Netherlands
Experimental methods	Cytokine production
Keywords

Notes

• The relationship between CTL escape and the subsequent increase in viral load and CD4 Th responses was studied in 2 patients. In both patients T-cell reactivity and recognition were lost after CTL escape, and in 1 patient only the loss of CTL responses was paralleled by a decrease in IL-2 CD4 Th responses.
• Autologous sequences corresponding to known and predicted Th epitopes were analyzed. KFDSRLAFHHMARELH had fixation of 2 mutations (KFDS[r/h]LAF[h/r]HMARELH) in 1 of the patients.

References

Geels2006 Mark J. Geels, Christine A. Jansen, Elly Baan, Iris M. De Cuyper, Gijs J. M. van Schijndel, Hanneke Schuitemaker, Jaap Goudsmit, Georgios Pollakis, Frank Miedema, William A. Paxton, and Debbie van Baarle. CTL Escape and Increased Viremia Irrespective of HIV-Specific CD4+ T-Helper Responses in Two HIV-Infected Individuals. Virology, 345(1):209-219, 5 Feb 2006. PubMed ID: 16246391. Show all entries for this paper.