Search T Helper/CD4+ T-Cell Epitope Database

Found 2 matching records:

Displaying record number 93

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HXB2 Location	Pol(683-698) RT(528-543) DNA(4131..4178)	Pol Epitope Map
Author Location	RT(528-543 BRU)
Epitope	KEKVYLAWVPAHKGIG	Epitope Alignment
Species (MHC/HLA)	mouse(H-2d, H-2f, H-2k)
Immunogen	vaccine
Experimental methods
Keywords

Vaccine Details

Notes

• T-cells from peptide-primed mice could be restimulated by native RT.

References

Haas1991 G. Haas, R. David, R. Frank, H. Gausepohl, C. Devaux, J.-M. Claverie, and M. Pierres. Identification of a Major Human Immunodeficiency Virus-1 Reverse Transcriptase Epitope Recognized by Mouse CD4+ T Lymphocytes. Eur. J. Immunol., 21:1371-1377, 1991. Notes: RT peptides were recognized by several of the T-helper lines established from RT-primed mice. Further, T-cells from peptide-primed mice could be restimulated by native RT. RT epitope: KEKVYLAWVPAHKGIG. PubMed ID: 1710563. Show all entries for this paper.

Displaying record number 202005

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HXB2 Location	Pol(683-698) RT(528-543) DNA(4131..4178)	Pol Epitope Map
Author Location	RT(528-543)
Epitope	KEKVYLAWVPAHKGIG	Epitope Alignment
Subtype	B
Species (MHC/HLA)	mouse
Immunogen	in vitro stimulation or selection, vaccine
Experimental methods	Cytokine production, FluoroSpot, Other
Keywords	vaccine-induced immune responses

Vaccine Details

Notes

• Two model HXB2-derived DNA immunogens, protease (PR) and reverse transcriptase (RT), were administered to Balb/c mice by intramuscular (IM) or intradermal (ID) single immunizations with subsequent electroporation revealing that ID delivery increased the production of IFNγ and IL-2 in PR-immunized, but not RT-immunized, splenocytes. A robust antibody response was only observed in RT-immunized mice and was significantly enhanced with ID, vs. IM, delivery. Epitopes PQVTLWQRPLVTIKI, AIGTVLVGPTPVNII, and KVVPLTNTTNQK also elicited in vitro responses in immunized splenocytes but it is less clear if this was predominantly CTL or CD4+ T cell-mediated recognition.
• After ID immunization with a plasmid containing RT, ˜0.2% of reactive CTLs and ˜0.5% of reactive CD4+ T cells were KEKVYLAWVPAHKGIG-specific and mostly produced TNFα.

References

Petkov2018 Stefan Petkov, Elizaveta Starodubova, Anastasia Latanova, Athina Kilpeläinen, Oleg Latyshev, Simons Svirskis, Britta Wahren, Francesca Chiodi, Ilya Gordeychuk, and Maria Isaguliants. DNA Immunization Site Determines the Level of Gene Expression and the Magnitude, but Not the Type of the Induced Immune Response. PLoS One, 13(6):e0197902, 2018. PubMed ID: 29864114. Show all entries for this paper.