Search T Helper/CD4+ T-Cell Epitope Database

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Displaying record number 201266

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HXB2 Location	Tat(41-55) DNA(5951..5995)	Tat Epitope Map
Author Location	Tat(41-55 LAI/IIIB)
Epitope	KALGISYGRKKRRQR	Epitope Alignment
Epitope Name	Tat 41-55
Subtype	B
Species (MHC/HLA)	human(DR11, DR13, DR3, DRB5)
Immunogen	in vitro stimulation or selection
Patient MHC/HLA	DRB1*04:04, DRB1*07:01, DRB4; DRB1*01:01, DRB1*13:02, DRB3; DRB1*11:01, DRB1*11:04, DRB3; DRB1*03:01, DRB1*15:01, DRB3, DRB5; DRB1*13:01, DRB1*15:01, DRB3, DRB5; DRB1*07:01, DRB1*09:01, DRB4; DRB1*11:01, DRB1*13:01, DRB3; DRB1*13:01, DRB1*15:01, DRB3, DRB5
Experimental methods	CD4 T-cell Elispot - IFNγ, HLA binding
Keywords	binding affinity, HLA cross-presentation, variant cross-reactivity

Notes

• CD4+ T-cell response to Tat and Vpr was evaluated in healthy donors. Most donors responded to both Tat and Vpr. In Tat, for 8 HLA-unrelated donors, only one peptide (KALGISYGRKKRRQR) primed all CD4+ responses, while responses to Vpr involved 6 different peptides, depending on the HLA-DR molecules of the donor.
• KALGISYGRKKRRQR from LAI/IIIB isolate has 20% frequency in clade B. Almost all T cells primed by this peptide also recognized the most frequent variant KgLGISYGRKKRRQR (50% frequency in clade B, also present in consensus A1, B, C, D, F1, F2). 3 more variants KgLGISYGsKKRRQR, KALGISnGRKKRRQR, KgLGIyYGRKKRRQR (10% frequency each) were recognized to a lesser extent.
• Based on binding data and experiments with HLA-DR transfected L cells, this peptide was restricted to DR11, DRB5 and DR3. Using EBV cell lines, it was suggested that DR13 may be a restricting HLA for Tat 41-55.

References

Castelli2008 Florence A. Castelli, Diane Houitte, Gaetan Munier, Natacha Szely, Alain Lecoq, Jean-Paul Briand, Sylviane Muller, and Bernard Maillere. Immunoprevalence of the CD4+ T-Cell Response to HIV Tat and Vpr Proteins Is Provided by Clustered and Disperse Epitopes, Respectively. Eur. J. Immunol., 38(10):2821-2831, Oct 2008. PubMed ID: 18828138. Show all entries for this paper.