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Displaying record number 201439

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HXB2 Location	Pol(202-219) RT(47-64) DNA(2688..2741)	Pol Epitope Map
Author Location	RT(202-219)
Epitope	ITKIGPENPYNTPIFAIK	Epitope Alignment
Subtype	C
Species (MHC/HLA)	baboon
Immunogen	vaccine
Country	South Africa
Experimental methods	Intracellular cytokine staining, T-cell Elispot
Keywords	vaccine-induced immune responses

Vaccine Details

Notes

• Immune responses in chacma baboons to candidate DNA and MVA vaccines are described. The vaccines express 5 genes (gag, RT, tat, nef, env) from HIV clade C.
• The vaccine induced strong and broad T-cell responses targeting all 5 genes, with a median of 12 targeted peptides per animal and without any single protein dominating the response. The vaccine induced strong IFN-γ and IL-2 responses with a balanced distribution in the CD8+ and CD4+ T-cell compartments. Mucosal immunity as well as long-lasting responses, present over a year after vaccination were induced.
• Responses were mapped with IFN-γ ELISPOT assay and where cells were available, the phenotype of the response was determined using intracellular cytokine staining.
• This peptide was targeted by 2 animals in ELISPOT and was phenotyped as CD4 response. It contains no previously determined CD4 epitopes.

References

Burgers2009 Wendy A. Burgers, Gerald K. Chege, Tracey L. Muller, Joanne H. van Harmelen, Greg Khoury, Enid G. Shephard, Clive M. Gray, Carolyn Williamson, and Anna-Lise Williamson. Broad, High-Magnitude and Multifunctional CD4+ and CD8+ T-Cell Responses Elicited by a DNA and Modified Vaccinia Ankara Vaccine Containing Human Immunodeficiency Virus Type 1 Subtype C Genes in Baboons. J. Gen. Virol., 90(Pt 2):468-480, Feb 2009. PubMed ID: 19141458. Show all entries for this paper.