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Displaying record number 201396

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HXB2 Location	gp160(404-420) gp120(404-420) DNA(7434..7484)	gp160 Epitope Map
Author Location	gp120
Epitope	GSNNTVGNPIILPCRI	Epitope Alignment
Subtype	A, B, C, D
Species (MHC/HLA)	human
Immunogen	vaccine
Country	United States
Experimental methods	Cytokine production
Keywords	subtype comparisons, immunodominance

Vaccine Details

Notes

• A vaccine study with B clade Envs in mice was unertaken to assess a subtype-specificity of responses. Four T-cell hybridomas responsive to subtype B envelope proteins were tested against 20 different subtype B envelope proteins and a protein each from subtypes A, C and D. IL-2 production was measured.
• No consistent correlation was found between T cell specificity towards epitopes from a certain (B) subtype or lack of specificity towards other (A, C, D) subtype.
• Not only did T-cell specificity not vary with subtype, but pairwise sequence comparisons of HIV gp120 envelope sequences showed that some US-derived sequences were more similar to sequences from distant countries than to each other
• Changes in core epitopes, flanking and distant regions, all affected responsiveness of the hybridomas to different subtype Env epitopes, showing that it is not only core changes that can eliminate T cell reactivity to an epitope.
• The above findings were substantiated by database analyses showing that epitope distributions are not necessarily dictated by subtype.
• Peptide GSNNTVGNPIILPCRI was the only one recognized by the hybridoma among multiple B, A, C and D variants, and it was the only one including the minimal core sequences NPIIL.

References

Brown2006 S. A. Brown, K. S. Slobod, S. Surman, A. Zirkel, X. Zhan, and J. L. Hurwitz. Individual HIV Type 1 Envelope-Specific T Cell Responses and Epitopes Do Not Segregate by Virus Subtype. AIDS Res. Hum. Retroviruses, 22(2):188-194, Feb 2006. PubMed ID: 16478402. Show all entries for this paper.