Search T Helper/CD4+ T-Cell Epitope Database

Found 3 matching records:

Displaying record number 201653

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HXB2 Location	Gag(269-283) p24(137-151) DNA(1594..1638)	Gag Epitope Map
Author Location	Gag
Epitope	`GLNKIVRMYSPVSIL`	Epitope Alignment
Epitope Name	Gag-GL15
Subtype	B
Species (MHC/HLA)	human
Immunogen	HIV-1 infection
Country	Sweden
Experimental methods	CD4 T-cell Elispot - IFNγ, Intracellular cytokine staining
Keywords	subtype comparisons, computational epitope prediction

Notes

47 computationally selected helper epitopes from Gag, Nef, Env, Pol, Tat, were found to induce HIV-1-specific T-cell responses in a 38-member cohort of subjects infected with 10 different HIV-1 subtypes. 13 subjects were not taking any treatment, and HLA restriction was to multiple -DR and -DQ alleles. The peptides identified were therefore able to provide immune coverage for both viral and genetic variations across diverse ethnic backgrounds and as expected, viral load (VL) correlated inversely to the breadth and polyfunctionality of HTL response to these peptides.
Gag peptide GLNKIVRMYSPVSIL is reported for the first time to be broadly immunogenic, and is predicted to interact with 15 HLA-DR/DQ alleles. 6/38 (15.8%) of subjects had a CD4+ response to Gag-GL15.

References

Buggert2012 Marcus Buggert, Melissa M. Norström, Chris Czarnecki, Emmanuel Tupin, Ma Luo, Katarina Gyllensten, Anders Sönnerborg, Claus Lundegaard, Ole Lund, Morten Nielsen, and Annika C. Karlsson. Characterization of HIV-Specific CD4+ T Cell Responses Against Peptides Selected with Broad Population and Pathogen Coverage. PLoS One, 7(7):e39874, 2012. PubMed ID: 22792193. Show all entries for this paper.

Displaying record number 201889

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HXB2 Location	Gag(269-283) p24(137-151) DNA(1594..1638)	Gag Epitope Map
Author Location	Gag
Epitope	`GLNKIVRMYSPVSIL`	Epitope Alignment
Epitope Name	Gag 17
Subtype	A, B
Species (MHC/HLA)	human
Immunogen	vaccine
Experimental methods	CD8 T-cell Elispot - IFNγ, Intracellular cytokine staining, Other
Keywords	vaccine-induced immune responses

Vaccine Details

Vaccine strain	A clade, B clade, C clade, D clade

Notes

Previous studies of the HIVconsv vaccine containing a chimeric protein with alternating HIV-1 Clade A, B, C, D conserved regions showed that subdominant epitopes could induce strong CTL response correlating with Gag and Pol-specific IFN-γ production. Here the specificity i.e. the epitopes themselves were characterized as well as the functionality, (i.e. cytokine production) of CTL from 3 subjects of the HIV-CORE 002 trial.
CTL elicited by HIVconsv from healthy human volunteer 418 (A*0201, A*2402/B*0702, B*2705/Cw*0102, Cw*0702), when expanded upon stimulation with peptide Gag 17, GLNKIVRMYSPVSIL (Gag 268-282), gave a strongly hierarchical immune response of 1583 sfc/ml.

References

Ahmed2016 Tina Ahmed, Nicola J. Borthwick, Jill Gilmour, Peter Hayes, Lucy Dorrell, and Tomáš Hanke. Control of HIV-1 Replication In Vitro by Vaccine-Induced Human CD8+ T Cells through Conserved Subdominant Pol Epitopes. Vaccine, 34(9):1215-1224, 24 Feb 2016. PubMed ID: 26784683. Show all entries for this paper.

Displaying record number 201947

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HXB2 Location	Gag(269-283) p24(137-151) DNA(1594..1638)	Gag Epitope Map
Author Location	Gag(269-283)
Epitope	`GLNKIVRMYSPVSIL`	Epitope Alignment
Epitope Name	HC017
Subtype	A, B
Species (MHC/HLA)	human
Immunogen	vaccine
Patient MHC/HLA	VID418: A02:01, A24:02, B07:02, B27:05, C01:02, C07:02, DQB103:01, DQB103:03, DRB104:01, DRB107:01, DRB4
Country	United Kingdom
Experimental methods	CD4 T-cell Elispot - IFNγ, Intracellular cytokine staining
Keywords	vaccine-induced immune responses, CD4+ CTL

Vaccine Details

Vaccine type	non-replicating adenovirus, polyepitope, DNA prime with modified vaccinia Ankara (MVA) boost, HIVconsv
Vaccine strain	A clade, B clade, multiple epitope immunogen, C clade, D clade
Vaccine component	Env, Gag, Pol, Vif, other

Notes

HIVconsv vaccine consists of 14 highly conserved regions of HIV-1 Gag, Pol, Vif, Env across clades A, B, C and D. Two vaccine regimens, CM or DDDCM, (ChAdV — ChAdV63.HIVconsv; MVA — MVA.HIVconsv; DNA - pSG2.HIVconsv) were used in the HIV-CORE 002 trial comprising HIV-negative, low-risk individuals. 199 15-mer peptides overlapping by 11 amino acids, which spanned the entire HIVconsv protein were used to interrogate PBMC from subjects, 11 of whom responded in CD4+ T cell EliSpot screenings. Truncation studies of specific epitopes suggested that the peptide length and sequence variation (altered peptide ligands) can determine whether a peptide elicits a full (agonist) or partial (partial agonist) activation phenotype or inhibitory (antagonist) CD4+ T-cell response to a stimulatory peptide epitope. Delivery of HIVconsv out of the context of the whole virus by this potent vaccine regimen revealed many novel T-cell epitopes not previously identified due to subdominant responses and thus not recorded in the Los Alamos Immunology HIV database before.
Volunteer ID 418 had a stimulatory response to 15-mer GLNKIVRMYSPVSIL as well as shorter peptides, LNKIVRMYSPVSIL (HC017A), GLNKIVRMYSPVSI (HC017B), NKIVRMYSPVSIL (HC017C), GLNKIVRMYSPVS (GS13 or HC017D), and KIVRMYSPVSIL (KL11 or HC017E). In addition to IFN-γ, TNF-α was also produced.
Epitope Location Finder tool predicts epitope cores at LNKIVRMYS for DRB1*0401 and IVRMYS for DRB1*0701, both of which are predicted HLA.

References

Borthwick2020 Nicola Borthwick, Sandra Silva-Arrieta, Anuska Llano, Masafumi Takiguchi, Christian Brander, and Tomáš Hanke. Novel Nested Peptide Epitopes Recognized by CD4+ T Cells Induced by HIV-1 Conserved-Region Vaccines. Vaccines, 8(1), 16 Jan 2020. PubMed ID: 31963212. Show all entries for this paper.