Search T Helper/CD4+ T-Cell Epitope Database

Found 4 matching records:

Displaying record number 201428

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HXB2 Location	Gag(259-273) p24(127-141) DNA(1564..1608)	Gag Epitope Map
Author Location	Gag(294-)
Epitope	GEIYKRWIILGLNKI	Epitope Alignment
Epitope Name	Gag294
Species (MHC/HLA)	transgenic mouse(DR)
Immunogen	vaccine
Experimental methods	CD4 T-cell Elispot - IFNγ
Keywords	vaccine-induced immune responses, vaccine antigen design

Vaccine Details

Notes

• A novel polypeptide vaccine of HIV T helper epitopes (EP-1043) and a DNA vaccine of HIV CTL epitopes was designed and tested for immunogenicity and safety in transgenic mice, rabbits and guinea pigs.
• A Phase I human trial is described in Jin2009.
• This peptide was immunogenic in H2bxd mice.

References

Walker2009 Leslie E. Walker, Lo Vang, Xuefei Shen, Brian D. Livingston, Penny Post, Alessandro Sette, C. Steven Godin, and Mark J. Newman. Design and Preclinical Development of a Recombinant Protein and DNA Plasmid Mixed Format Vaccine to Deliver HIV-Derived T-Lymphocyte Epitopes. Vaccine, 27(50):7087-7095, 23 Nov 2009. PubMed ID: 19786132. Show all entries for this paper.

Displaying record number 398

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HXB2 Location	Gag(259-273) p24(127-141) DNA(1564..1608)	Gag Epitope Map
Author Location	Gag(294-308)
Epitope	GEIYKRWIILGLNKI	Epitope Alignment
Epitope Name	Gag 294
Species (MHC/HLA)	human(DR supermotif)
Immunogen	HIV-1 infection
Experimental methods
Keywords	subtype comparisons

Notes

• Eleven peptides were identified that had the HLA-DR supermotif, all were found to bind to MHC class II DR molecules and all elicted proliferative responses from multiple HIV-infected donors.
• This epitope binds ten HLA-DR alleles: DRB1*0101, DRB1*1501, DRB1*0405, DRB1*1101, DRB1*1302, DRB1*0701, DRB1*0802, DRB1*0901, DRB5*0101 and DRB4*0101 with an IC₅₀ threshold below 1,000 nM.
• This epitope sequence is conserved in 95% of clade B isolates.
• 6/22 HIV infected individuals responded to this epitope (13/22 responded to some of the DR supermotif epitopes, the 9 non-responder peptides tended to also not have recall responses to rec HIV-1 whole proteins)

References

Wilson2001 C. C. Wilson, B. Palmer, S. Southwood, J. Sidney, Y. Higashimoto, E. Appella, R. Chesnut, A. Sette, and B. D. Livingston. Identification and antigenicity of broadly cross-reactive and conserved human immunodeficiency virus type 1-derived helper T-lymphocyte epitopes. J. Virol., 75(9):4195-207, May 2001. URL: http://jvi.asm.org/cgi/content/full/75/9/4195. PubMed ID: 11287569. Show all entries for this paper.

Displaying record number 200935

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HXB2 Location	Gag(259-273) p24(127-141) DNA(1564..1608)	Gag Epitope Map
Author Location	p24(294-308)
Epitope	GEIYKRWIILGLNKI	Epitope Alignment
Epitope Name	Gag2
Species (MHC/HLA)	human(DR supermotif)
Immunogen	HIV-1 infection
Country	United Kingdom
Experimental methods	Cytokine production, Proliferation
Keywords	supertype, rate of progression

Notes

• Proliferative and cytokine (IFNgamma and Il-2) immune responses to 11 previously defined (Wilson 2001), and immunodominant and broadly cross-reactive Th epitopes and to p24 overlapping peptides were characterized in 10 LTNPs (chronically infected, asymptomatic, long term non-progressors, CD4+ T cells >500 after 15 years) and 7 SPs (slow progressors, CD4+ T cell declined to <500 after 15 years. Patients were treatment-naive.
• Gag-specific CD4+ T cells in LTNPs showed increased numbers of IFN gamma and IL-2 producing cells compared to SPs. Cytokine production and proliferative responses were negatively correlated with the viral load and positively correlated with the CD4+ T cell count. IL-2, but not IFNgamma, levels were correlated with proliferation.

References

Boaz2003 M. J. Boaz, A. Waters, S. Murad, P. J. Easterbrook, E. D'Sousa, C. van Wheeley, and A. Vyakarnam. CD4 Responses to Conserved HIV-1 T Helper Epitopes Show Both Negative and Positive Associations with Virus Load in Chronically Infected Subjects. Clin. Exp. Immunol., 134(3):454-463, Dec 2003. PubMed ID: 14632751. Show all entries for this paper.

Displaying record number 201713

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HXB2 Location	Gag(259-273) p24(127-141) DNA(1564..1608)	Gag Epitope Map
Author Location	Gag(259-273 LAI)
Epitope	GEIYKRWIILGLNKI	Epitope Alignment
		Show epitope variants
Epitope Name	Gag 259-273
Subtype	B, C
Species (MHC/HLA)	human
Immunogen	vaccine
Experimental methods	CD4 T-cell Elispot - IFNγ, HLA binding
Keywords	vaccine-induced immune responses, variant cross-reactivity

Vaccine Details

Notes

• 22 seronegative subjects were evaluated for production of Lipo5 vaccine-specific naive HTL when stimulated with one of five HIV protein fragments that make up the vaccine. Not only was there good cross-reactivity between clade B and C peptide variants tested, but 4/5 Lipo5 peptides were able to bind HLA-DR molecules. G2 was the vaccine peptide that most elicited a specific-HTL repertoire, while both G2 and P1 were able to elicit a response in HTL that were HIV-infected.
• Gag 15-mer peptide, Gag 259-273, was the minimal peptide recognized by 9% of mono-specific T cell lines that were also G2-specific. 2 poly-specific HTL lines also recognized Gag 259-273.
• Gag 259-273 substitution - L268M - weakly affected HTL-activation. Substitution at variable position E260D however, greatly affected HTL activation with a reduction in cross-reaction to <50%. Gag 259-273 was present in 68.15% of clade B sequences.

References

Castelli2013 Florence Anne Castelli, Natacha Szely, Alexis Olivain, Nicoletta Casartelli, Caroline Grygar, Aurélie Schneider, Aurore Besse, Yves Levy, Olivier Schwartz, and Bernard Maillère. Hierarchy of CD4 T Cell Epitopes of the ANRS Lipo5 Synthetic Vaccine Relies on the Frequencies of Pre-Existing Peptide-Specific T Cells in Healthy Donors. J. Immunol., 190(11):5757-5763, 1 Jun 2013. PubMed ID: 23636059. Show all entries for this paper.