Found 1 matching record:
Displaying record number 201417
Download this epitope
record as JSON.
- IL-2 producing CD4 T cells were studied in 3 acutely infected HIV-1 infected subjects. Despite the early presence of IL-2 producing T cells, each subject exhibited progressive disease, one subject showing rapid progression. In this rapid progressor, 3 IL-2 producing CD4 T-cell responses were identified, mapped to optimal epitopes and further studied.
- FRDYVDRFYKTLRAE was targeted by IL-2 producing T cell clone in subject OM255 (44 years old male, viral load 18,322 copies/ml), sampled estimated 46 days postinfection and in rapid progressor, subject OM214 (43 years old male, viral load >400,00 copies/ml), sampled estimated 60 days postinfection. Responses in a rapid progressor, subject OM214, were further studied.
- For the rapid progressor 3 CD4+ T-cell clones were obtained and fine-mapped by EliSpot using truncated peptides. FRDYVDRFYKT was the optimal epitope for FRDYVDRFYKTLRAE.
- Low frequency IL-2 producing response to FRDYVDRFYKT was present at 2 months postinfection but not detected at 12 months postinfection. The variant FRDYVDqFYKT was present at 5 and 7 months postinfection. It failed to elicit a response as measured by IL-2 production, degranulation (CD107a staining) or INF-γ production, thus constituting an escape variant. This escape mutant did not antagonize responses to the index peptide and mutations flanking the epitope did not impact recognition.
- HLA restriction DQA1*01/DQB1*05 was determined by testing a panel of allogeneic BLCL matched with subject OM214 on at least one DQ allele for the ability to present FRDYVDRFYKT.
R. Brad Jones, Feng-Yun Yue, Xiao Xiao Jenny Gu, Diana V. Hunter, Shariq Mujib, Gabor Gyenes, Rosemarie D. Mason, Ruqaya Mohamed, Kelly S. MacDonald, Colin Kovacs, and Mario A. Ostrowski. Human Immunodeficiency Virus Type 1 Escapes from Interleukin-2-Producing CD4+ T-Cell Responses without High-Frequency Fixation of Mutations. J. Virol., 83(17):8722-8732, Sep 2009. PubMed ID: 19553327.
Show all entries for this paper.