Search T Helper/CD4+ T-Cell Epitope Database

Found 2 matching records:

Displaying record number 201888

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HXB2 Location	Gag(167-181) p24(35-49) DNA(1288..1332)	Gag Epitope Map
Author Location	Gag
Epitope	EVIPMFTALSEGATP	Epitope Alignment
Epitope Name	Gag 3
Subtype	A, B
Species (MHC/HLA)	human
Immunogen	vaccine
Experimental methods	CD8 T-cell Elispot - IFNγ, Intracellular cytokine staining, Other
Keywords	vaccine-induced immune responses

Vaccine Details

Notes

• Previous studies of the HIVconsv vaccine containing a chimeric protein with alternating HIV-1 Clade A, B, C, D conserved regions showed that subdominant epitopes could induce strong CTL response correlating with Gag and Pol-specific IFN-γ production. Here the specificity i.e. the epitopes themselves were characterized as well as the functionality, (i.e. cytokine production) of CTL from 3 subjects of the HIV-CORE 002 trial.
• CTL elicited by HIVconsv from healthy human volunteer 418 (A*0201, A*2402/B*0702, B*2705/Cw*0102, Cw*0702), when expanded upon stimulation with peptide Gag 3, EVIPMFTALSEGATP (Gag 166-171), gave a strongly hierarchical immune response of 1200 sfc/ml.

References

Ahmed2016 Tina Ahmed, Nicola J. Borthwick, Jill Gilmour, Peter Hayes, Lucy Dorrell, and Tomáš Hanke. Control of HIV-1 Replication In Vitro by Vaccine-Induced Human CD8+ T Cells through Conserved Subdominant Pol Epitopes. Vaccine, 34(9):1215-1224, 24 Feb 2016. PubMed ID: 26784683. Show all entries for this paper.

Displaying record number 201948

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HXB2 Location	Gag(167-181) p24(35-49) DNA(1288..1332)	Gag Epitope Map
Author Location	Gag(167-181)
Epitope	EVIPMFTALSEGATP	Epitope Alignment
Epitope Name	HC003
Species (MHC/HLA)	human
Immunogen	vaccine
Patient MHC/HLA	VID418: A*02:01, A*24:02, B*07:02, B*27:05, C*01:02, C*07:02, DQB1*03:01, DQB1*03:03, DRB1*04:01, DRB1*07:01, DRB4
Country	United Kingdom
Experimental methods	CD4 T-cell Elispot - IFNγ, Intracellular cytokine staining
Keywords	vaccine-induced immune responses, CD4+ CTL

Vaccine Details

Notes

• HIVconsv vaccine consists of 14 highly conserved regions of HIV-1 Gag, Pol, Vif, Env across clades A, B, C and D. Two vaccine regimens, CM or DDDCM, (ChAdV — ChAdV63.HIVconsv; MVA — MVA.HIVconsv; DNA - pSG2.HIVconsv) were used in the HIV-CORE 002 trial comprising HIV-negative, low-risk individuals. 199 15-mer peptides overlapping by 11 amino acids, which spanned the entire HIVconsv protein were used to interrogate PBMC from subjects, 11 of whom responded in CD4+ T cell EliSpot screenings. Truncation studies of specific epitopes suggested that the peptide length and sequence variation (altered peptide ligands) can determine whether a peptide elicits a full (agonist) or partial (partial agonist) activation phenotype or inhibitory (antagonist) CD4+ T-cell response to a stimulatory peptide epitope. Delivery of HIVconsv out of the context of the whole virus by this potent vaccine regimen revealed many novel T-cell epitopes not previously identified due to subdominant responses and thus not recorded in the Los Alamos Immunology HIV database before.
• Volunteer ID 418 had a stimulatory response to 15-mer EVIPMFTALSEGATP as well as shorter peptides, EVIPMFTALSEGAT (ET14/HC003B), EVIPMFTALSEGA (EA13/HC003D), EVIPMFTALSEG (EG12/HC003F), EVIPMFTALSE (EE11/HC003H), and EVIPMFTALS (ES10/HC003J). In addition to IFN-γ, TNF-α was also produced.
• Epitope Location Finder tool predicts epitope cores at EVIPMFTALS and FTALSEGAT for DRB1*0401 and VIPMPT and LSEGAT for DRB1*0701, both of which are predicted HLA.

References

Borthwick2020 Nicola Borthwick, Sandra Silva-Arrieta, Anuska Llano, Masafumi Takiguchi, Christian Brander, and Tomáš Hanke. Novel Nested Peptide Epitopes Recognized by CD4+ T Cells Induced by HIV-1 Conserved-Region Vaccines. Vaccines, 8(1), 16 Jan 2020. PubMed ID: 31963212. Show all entries for this paper.