Search T Helper/CD4+ T-Cell Epitope Database

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Displaying record number 201144

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HXB2 Location	Rev(11-27) DNA(6000..6045,8379..8383)	Rev Epitope Map
Author Location	Rev(11-276 clade B consensus)
Epitope	ELLKTVRLIKFLYQSNP	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human(DRB1*01:01, DRB1*03:01, DRB1*04:01, DRB1*04:05, DRB1*07:01, DRB1*11:01, DRB1*13:02, DRB1*15:01)
Immunogen	HIV-1 infection
Country	Brazil
Experimental methods	CD4 T-cell Elispot - IFNγ, HLA binding
Keywords	binding affinity, computational epitope prediction, HLA cross-presentation, chronic infection

Notes

• TEPITOPE algorithm was used to screen the most conserved regions of clade B consensus sequence for multiple HLA-DR binding epitopes. Peptides predicted to bind 16 or more HLA-DR molecules were synthesized and further assessed in binding assays and for T-cell recognition in a cohort of 32 Brazilian HIV-1 chronically infected individuals.
• While the reacting peptide was ELLKTVRLIKFLYQSNP, shorter LLKTVRLIKFLYQ peptide was predicted by TEPITOPE to bind HLA-DR molecules. Flanking amino acids were added to increase the efficiency of in vitro peptide presentation to CD4 T cells.

References

Fonseca2006 Simone G Fonseca, Adriana Coutinho-Silva, Luiz Augusto M. Fonseca, Aluisio C. Segurado, Sandra L. Moraes, Hélcio Rodrigues, Juergen Hammer, Esper G. Kallás, John Sidney, Alessandro Sette, Jorge Kalil, and Edecio Cunha-Neto. Identification of Novel Consensus CD4 T-Cell Epitopes from Clade B HIV-1 Whole Genome That Are Frequently Recognized by HIV-1 Infected Patients. AIDS, 20(18):2263-2273, 28 Nov 2006. PubMed ID: 17117012. Show all entries for this paper.