Search T Helper/CD4+ T-Cell Epitope Database

Found 5 matching records:

Displaying record number 201432

Download this epitope record as JSON.

HXB2 Location	Pol(684-698) RT(529-543) DNA(4134..4178)	Pol Epitope Map
Author Location	Pol(711-)
Epitope	`EKVYLAWVPAHKGIG`	Epitope Alignment
Epitope Name	Pol711
Species (MHC/HLA)	transgenic mouse(DR)
Immunogen	vaccine
Experimental methods	CD4 T-cell Elispot - IFNγ
Keywords	vaccine-induced immune responses, vaccine antigen design

Vaccine Details

Vaccine type	polyepitope
Vaccine component	Env, Gag, Pol, Vpu

Notes

A novel polypeptide vaccine of HIV T helper epitopes (EP-1043) and a DNA vaccine of HIV CTL epitopes was designed and tested for immunogenicity and safety in transgenic mice, rabbits and guinea pigs.
A Phase I human trial is described in Jin2009.
This peptide was immunogenic in H2bxd mice.

References

Walker2009 Leslie E. Walker, Lo Vang, Xuefei Shen, Brian D. Livingston, Penny Post, Alessandro Sette, C. Steven Godin, and Mark J. Newman. Design and Preclinical Development of a Recombinant Protein and DNA Plasmid Mixed Format Vaccine to Deliver HIV-Derived T-Lymphocyte Epitopes. Vaccine, 27(50):7087-7095, 23 Nov 2009. PubMed ID: 19786132. Show all entries for this paper.

Displaying record number 50539

Download this epitope record as JSON.

HXB2 Location	Pol(684-698) RT(529-543) DNA(4134..4178)	Pol Epitope Map
Author Location	Pol(711-725)
Epitope	`EKVYLAWVPAHKGIG`	Epitope Alignment
Epitope Name	Pol 711
Species (MHC/HLA)	mouse(DR, I-A^b)
Immunogen	vaccine
Patient MHC/HLA	H-2b
Experimental methods
Keywords	vaccine-induced immune responses, immunodominance

Vaccine Details

Vaccine type	DNA with CMV promotor, peptide
Adjuvant	Complete Freund's Adjuvant (CFA)

Notes

4 Th HIV epitopes presented by HLA-DR molecules were identified that also could be presented by murine class II molecule I-Ab, enabling testing of vaccine strategies in H-2b mice.
Responses to pooled peptides, polyepitope peptides in a linear construct or in a branched MAP construct, and a DNA polyepitope construct with a CMV promoter were compared. A linear arrangement in polyepitope construct created a junctional epitope that could be disrupted with the addition of GPGPG spacers. The linear polyepitope construct with the GPGPG spacer worked well in terms of eliciting responses to all 4 peptides, using either DNA or protein for the vaccination.
Although responses to this peptide indicated it was immunodominant, responses to all 4 peptides were made upon vaccination with linear constructs when GPGPG spacers were used.

References

Livingston2002 Brian Livingston, Claire Crimi, Mark Newman, Yuichiro Higashimoto, Ettore Appella, John Sidney, and Alessandro Sette. A Rational Strategy to Design Multiepitope Immunogens Based on Multiple Th Lymphocyte Epitopes. J. Immunol., 168(11):5499-5506, 1 Jun 2002. PubMed ID: 12023344. Show all entries for this paper.

Displaying record number 405

Download this epitope record as JSON.

HXB2 Location	Pol(684-698) RT(529-543) DNA(4134..4178)	Pol Epitope Map
Author Location	Pol(711-725)
Epitope	`EKVYLAWVPAHKGIG`	Epitope Alignment
Epitope Name	Pol 711
Species (MHC/HLA)	human(DR supermotif)
Immunogen	HIV-1 infection
Experimental methods
Keywords	subtype comparisons

Notes

Eleven peptides were identified that had the HLA-DR supermotif, all were found to bind to MHC class II DR molecules and all elicited proliferative responses from multiple HIV-infected donors.
This epitope binds ten HLA-DR alleles: DRB1*0101, DRB1*1501, DRB1*0401, DRB1*0405, DRB1*1101, DRB1*0701, DRB1*0802, DRB1*0901, DRB5*0101 and DRB4*0101, with an IC₅₀ threshold below 1,000 nM.
This epitope sequence is conserved in 94% of clade B isolates.
6/22 HIV infected individuals responded to this epitope (13/22 responded to some of the DR supermotif epitopes, the 9 non-responder peptides tended to also not have recall responses to rec HIV-1 whole proteins)

References

Wilson2001 C. C. Wilson, B. Palmer, S. Southwood, J. Sidney, Y. Higashimoto, E. Appella, R. Chesnut, A. Sette, and B. D. Livingston. Identification and antigenicity of broadly cross-reactive and conserved human immunodeficiency virus type 1-derived helper T-lymphocyte epitopes. J. Virol., 75(9):4195-207, May 2001. URL: http://jvi.asm.org/cgi/content/full/75/9/4195. PubMed ID: 11287569. Show all entries for this paper.

Displaying record number 200938

Download this epitope record as JSON.

HXB2 Location	Pol(684-698) RT(529-543) DNA(4134..4178)	Pol Epitope Map
Author Location	Protease-RT(711-725)
Epitope	`EKVYLAWVPAHKGIG`	Epitope Alignment
Epitope Name	Pol4
Species (MHC/HLA)	human(DR supermotif)
Immunogen	HIV-1 infection
Experimental methods	Intracellular cytokine staining, Proliferation
Keywords	rate of progression, superinfection

Notes

Proliferative and cytokine (IFNgamma and Il-2) immune responses to 11 previously defined (Wilson 2001), and immunodominant and broadly cross-reactive Th epitopes and to p24 overlapping peptides were characterized in 10 LTNPs (chronically infected, asymptomatic, long term non-progressors, CD4+ T cells >500 after 15 years) and 7 SPs (slow progressors, CD4+ T cell declined to <500 after 15 years. Patients were treatment-naive.
Gag-specific CD4+ T cells in LTNPs showed increased numbers of IFN gamma and IL-2 producing cells compared to SPs. Cytokine production and proliferative responses were negatively correlated with the viral load and positively correlated with the CD4+ T cell count. IL-2, but not IFNgamma, levels were correlated with proliferation.

References

Boaz2003 M. J. Boaz, A. Waters, S. Murad, P. J. Easterbrook, E. D'Sousa, C. van Wheeley, and A. Vyakarnam. CD4 Responses to Conserved HIV-1 T Helper Epitopes Show Both Negative and Positive Associations with Virus Load in Chronically Infected Subjects. Clin. Exp. Immunol., 134(3):454-463, Dec 2003. PubMed ID: 14632751. Show all entries for this paper.

Displaying record number 201836

Download this epitope record as JSON.

HXB2 Location	Pol(684-698) RT(529-543) DNA(4134..4178)	Pol Epitope Map
Author Location	Pol
Epitope	`EKVYLAWVPAHKGIG`	Epitope Alignment
Epitope Name	Pol₇₁₁
Species (MHC/HLA)	mouse
Immunogen	vaccine
Experimental methods	Other, Proliferation
Keywords	vaccine-induced immune responses, vaccine antigen design, junction or fusion peptide, Synthetic peptide

Notes

T-helper eptiopes, Pan DR epitope (PADRE) and Pol₇₁₁ were grafted into the cysteine-rich domain (CRD) and/or the basic domain (BD) of Tat protein to abolish its transactivation potential, making it safer as a vaccine. Enhanced antibody response was detected.
Pol₇₁₁, EKVYLAWVPAHKGIG, was grafted into Tat peptides, boosting interaction with antibodies against Tat.
Junction peptide E, ACNNCYCKRCEKVYLAWVPAHKGIG, was an HTL Tat peptide with Pol₇₁₁ grafted into its CRD that elicited greater antibody response as well as Th1/Th2 responses in mice.
Junction peptide F, EKVYLAWVPAHKGIGSYHCLVCFQT/K, was an HTL Tat peptide with Pol₇₁₁ grafted into its CRD that elicited greater antibody response as well as Th1/Th2 responses in mice.
Junction peptide G, GLGIC/GYGRKKEKVYLAWVPAHKGIG, was an HTL Tat peptide with Pol₇₁₁ grafted into its BD that elicited greater antibody response as well as Th1/Th2 responses in mice.
Junction peptide H, EKVYLAWVPAHKGIGRRQRRSAPPS, was an HTL Tat peptide with Pol₇₁₁ grafted into its BD that elicited greater antibody response as well as Th1/Th2 responses in mice.

References

Kashi2014 Venkatesh P. Kashi, Rajesh A. Jacob, Raghavendra A. Shamanna, Malini Menon, Anangi Balasiddaiah, Rebu K. Varghese, Mahesh Bachu, and Udaykumar Ranga. The Grafting of Universal T-Helper Epitopes Enhances Immunogenicity of HIV-1 Tat Concurrently Improving Its Safety Profile. PLoS One, 9(12):e114155, 2014. PubMed ID: 25531437. Show all entries for this paper.