Search T Helper/CD4+ T-Cell Epitope Database

Found 2 matching records:

Displaying record number 200876

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HXB2 Location	gp160(210-218) gp120(210-218) DNA(6852..6878)	gp160 Epitope Map
Author Location	Env(186-194 1035)
Epitope	FEPIPIHYC	Epitope Alignment
Subtype	B
Species (MHC/HLA)	mouse
Immunogen	vaccine
Experimental methods	Proliferation, T-cell Elispot
Keywords	epitope processing, vaccine-induced immune responses, escape, TCR usage, optimal epitope

Vaccine Details

Notes

• A very narrow Th response was stimulated in C57BL/6 mice vaccinated with vaccinia expressed HIV-1 env clone 1035. Five of seven different Th hybridomas isolated from five immunized mice immunized reacted with the peptide PKVSFEPIPIHYCAP, located in the C2 region of gp120. TCR Vβ usage indicated each of the clones was unique. Splenic populations from other C57BL/6 mice immunized with 1035 env confirmed that the gp120 specific T-helper response was focused on the PKVSFEPIPIHYCAP peptide. The authors suggest the protein structural context may contribute to the immunodominance of this peptide.
• The minimal epitope was mapped for one of the hybridomas, and was FEPIPIHYC.
• The natural variant, fDpipihyc, did not stimulate a response in three of the hybridomas.

References

Zhan2003 X. Zhan, K. S. Slobod, S. Surman, S. A. Brown, T. D. Lockey, C. Coleclough, P. C. Doherty, and J. L. Hurwitz. Limited Breadth of a T-Helper Cell Response to a Human Immunodeficiency Virus Envelope Protein. J. Virol., 77(7):4231-4236, Apr 2003. PubMed ID: 12634380. Show all entries for this paper.

Displaying record number 200877

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HXB2 Location	gp160(606-620) gp41(95-109) DNA(8040..8084)	gp160 Epitope Map
Author Location	gp41( 1035)
Epitope	TNVPWNASWSNKSLE	Epitope Alignment
Subtype	B
Species (MHC/HLA)	mouse
Immunogen	vaccine
Experimental methods	T-cell Elispot
Keywords	epitope processing, vaccine-induced immune responses, escape, TCR usage

Vaccine Details

Notes

• A very narrow Th response was stimulated in C57BL/6 mice vaccinated with vaccinia expressed HIV-1 env clone 1035, to the peptide PKVSFEPIPIHYCAP, located in the C2 region of gp120. The only other peptide recognized using Elispot on Env overlapping peptides to test vaccine responses in the mice was this one: TNVPWNASWSNKSLE, located in gp41.

References

Zhan2003 X. Zhan, K. S. Slobod, S. Surman, S. A. Brown, T. D. Lockey, C. Coleclough, P. C. Doherty, and J. L. Hurwitz. Limited Breadth of a T-Helper Cell Response to a Human Immunodeficiency Virus Envelope Protein. J. Virol., 77(7):4231-4236, Apr 2003. PubMed ID: 12634380. Show all entries for this paper.