Found 4 matching records:
Displaying record number 399
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Notes
- 11/16 heavily HIV exposed but persistently seronegative sex-workers in Nairobi had HIV-specific CD8 IFNγ responses in the cervix---systemic CD8+ T cell responses tended to be to the same epitopes but at generally lower levels than cervical CD8+ T cell responses.
- Low risk individuals did not have such CD8+ cells.
- CD8+ T cell epitopes: DTVLEDINL (3 individuals), SLYNTVATL (4 individuals), LSPRTLNAW (3 individuals) and YPLTFGWCF (4 individuals) were most commonly recognized by the HIV-resistant women.
References
Kaul2000
R. Kaul, F. A. Plummer, J. Kimani, T. Dong, P. Kiama, T. Rostron, E. Njagi, K. S. MacDonald, J. J. Bwayo, A. J. McMichael, and S. L. Rowland-Jones. HIV-1-Specific Mucosal CD8+ Lymphocyte Responses in the Cervix of HIV-1-Resistant Prostitutes in Nairobi. J. Immunol., 164:1602-1611, 2000. PubMed ID: 10640781.
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Displaying record number 1320
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Notes
- This study examines CTL responses in HIV exposed, persistently seronegative individuals, HEPS, who eventually seroconverted -- 11/114 HEPS Nairobi sex workers eventually seroconverted, and for six of these HIV CTL reactive epitopes had been defined while seronegative.
- DTVLEDINL was recognized in 3 of the 6 women (ML857, ML1203, and ML1707), and the response was present in the last available sample prior to seroconversion, 3-7 months.
- In each of the three women, 20/20 sequences of the infecting strain had no substitutions in this epitope, all were DTVLEDINL, so there was no evidence for escape.
- The epidemiological factor associated with seroconversion was stopping sex work. HIV-specific CTL activity declined when HEPS sex workers stopped working for a period or retired.
- This epitope was recognized in 3/22 HEPS sex worker controls, ML851, ML1432, and ML1601.
References
Kaul2001
R. Kaul, S. L. Rowland-Jones, J. Kimani, T. Dong, H. B. Yang, P. Kiama, T. Rostron, E. Njagi, J. J. Bwayo, K. S. MacDonald, A. J. McMichael, and F. A. Plummer. Late Seroconversion in HIV-Resistant Nairobi Prostitutes Despite Pre-Existing HIV-Specific CD8+ Responses. J. Clin. Invest., 107(3):341-349, Feb 2001. URL: http://www.jci.org/cgi/content/full/107/3/341. PubMed ID: 11160158.
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Displaying record number 1665
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Notes
- ELISPOT was used to study CTL responses to a panel of 54 predefined HIV-1 epitopes in 91 HIV-1-exposed, persistently seronegative (HEPS) and 87 HIV-1-infected female Nairobi sex workers.
- Responses in HEPS women tended to be lower, and focused on different epitopes with HLA presenting molecules that have previously been associated with reduced risk of infection, and there was a shift in the response in the HEPS women upon late seroconversion to epitopes recognized by the HIV-1 infected women.
- 43/91 HEPS women had CD8+ responses and detection of HIV-1-specific CTL in HEPS women increased with the duration of viral exposure.
- Among HLA-A*6802 women, 11/12 HEPS and 6/11 HIV-1 infected women recognized this epitope likelihood ratio 4.4, p value 0.08, and HEPS women tended to respond to DTVLEDINL, infected women tended to ETAYFILKL.
- The dominant response to this HLA allele was to this epitope in 10 of the 11/12 HEPS cases, but in only 4 of the 6/11 HIV-1 infected women.
- Differences in epitope specificity were only seen for responses restricted by class I HLA alleles A2, A24, A*6802, B14, and B18, previously shown to be associated with resistance to HIV-1 in this cohort.
- Four epitopes were considered to be ``resistant epitopes'', as they were preferentially reactive in HEPS women and so may confer resistance, and these were found in three different proteins: A2 ILK(D/E)PVHGV in RT, A*6802 DTVLEDINL in Protease, B14 DLN(M/T)LN(I/V)V in p24 and B18 FRDYVDRF(Y/F)K also in p24.
- Subject ML 857 shifted from a A*6802 DTVLEDINL and B35 (H/N)PDIVIYQY response prior to seroconversion to a B35 PPIPVGDIY and B35 VPLRPMTY response post-seroconversion, and the loss of the pre-seroconversion response was not due to sequence variation within these epitopes.
- Subject ML 1203 started with CTL responses to A*6802 DTVLEDINL and B7 FPVTPQVPLR prior to seroconversion, and upon seroconversion acquired additional responses to A*6802 ETAYFILKL which became dominant, B7 TPGPGV/IRYPL, B7 IPRRIRQGL, and B7 SPRTLNAWV.
- Subject ML 1707 started with a CTL response to A*6802 DTVLEDINL prior to seroconversion, and switched to A*6802 ETAYFILKL and A24 RDYVDRFFKTL post-seroconversion, and the loss of the pre-seroconversion response was not due to sequence variation within the epitope.
- Subject ML 1830 made no detectable response prior to seroconversion, but responded to A*6802 DTVLEDINL and A*6802 ETAYFILKL post-seroconversion.
References
Kaul2001a
R. Kaul, T. Dong, F. A. Plummer, J. Kimani, T. Rostron, P. Kiama, E. Njagi, E. Irungu, B. Farah, J. Oyugi, R. Chakraborty, K. S. MacDonald, J. J. Bwayo, A. McMichael, and S. L. Rowland-Jones. CD8(+) lymphocytes respond to different HIV epitopes in seronegative and infected subjects. J. Clin. Invest., 107(10):1303-10, May 2001. URL: http://www.jci.org/cgi/content/full/107/10/1303. PubMed ID: 11375420.
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Displaying record number 52202
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- Neisseria gonorrhea cervititis in 9 HIV+ Kenyan sex workers caused a functional deficiency in IFN-gamma production in HIV-1 epitope-specific CD8+ T-cells, detected by intracellular cytokine production and tetramer assays, while not affecting the total number of epitope-specific CTLs.
- Ghonorrhea caused the weaker HIV-1 specific CTL responses in 4 HIV-1 exposed persistently seronegative (HEPS) women to become undetectable by Elispot and tetramer assays, and CMV-specific CTL in 2 HEPS subjects were shown to have impaired function with regard to IFN-gamma production.
References
Kaul2002
Rupert Kaul, Sarah L. Rowland-Jones, Geraldine Gillespie, Joshua Kimani, Tao Dong, Peter Kiama, J. Neil Simonsen, Job J. Bwayo, Andrew J. McMichael, and Francis A. Plummer. Gonococcal Cervicitis is Associated with Reduced Systemic CD8+ T Cell Responses in Human Immunodeficiency Virus Type 1-Infected and Exposed, Uninfected Sex Workers. J. Infect. Dis., 185(10):1525-1529, 15 May 2002. PubMed ID: 11992292.
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