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Displaying record number 58383

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HXB2 Location	Gag(42-56) p17(42-56) DNA(913..957)	Gag Epitope Map
Author Location	Gag
Epitope	ERFALNPGLLETSEG	Epitope Alignment
		Show epitope variants
Epitope Name	Peptide 11
Subtype	C
Species (MHC/HLA)	human
Immunogen	HIV-1 infection
Patient MHC/HLA	A*02:01, A*30:02, B*08:01:01, B*45:01, C*16:01
Country	China, South Africa
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	subtype comparisons, variant cross-reactivity, vaccine antigen design

Notes

• 39 South African clade-C-infected subjects were tested for IFN-γ responses to 5 OLP setsfrom clades A, B, D and matching consensus sets from clade C strains in China and South Africa. Responses to clade-C peptide sets did not differ in breadth and magnitude (intra-clade recognition), but were recognized significantly more than other clades' (inter-clade recognition). 13/19 C-clade recognized peptides were common between the peptide sets C_Du422 from South Africa and C_CH from China.
• Within Gag, a few highly conserved epitopes were found to be the regions where most inter-clade reactivity occurred i.e. 17/84 recognized peptides, were recognized from clades A, B, C, D by the C-infected subjects, and these peptides had lowest entropy. Peptides recognized that were from single clades however, had higher entropy scores. 7/17 peptides were in Gag p17 and 10/17 were in Gag p24.
• Computer predictions of epitope processing (proteasomal cleavage scores) and epitope presentation (MHC I binding scores) suggest that host immune selection pressure is responsible for the lack of peptide cross-recognition due to genetic divergence between clades.
• Peptide 11 (South Africa), ERFALNPGLLETSEG, containing predicted epitope ALNPGLLET, elicited CTL response in one subject whose autologous clade-C HIV-1 sequence was EkFALNPGLLETSdG.

References

Zembe2011 Lycias Zembe, Wendy A. Burgers, Heather B. Jaspan, Linda-Gail Bekker, Helba Bredell, Gwynneth Stevens, Jill Gilmour, Josephine H. Cox, Patricia Fast, Peter Hayes, Eftyhia Vardas, Carolyn Williamson, and Clive M. Gray. Intra- and Inter-Clade Cross-Reactivity by HIV-1 Gag Specific T-Cells Reveals Exclusive and Commonly Targeted Regions: Implications for Current Vaccine Trials. PLoS One, 6(10):e26096, 2011. PubMed ID: 22022524. Show all entries for this paper.