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Displaying record number 55092

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HXB2 Location	Gag(162-172) p24(30-40) DNA(1273..1305)	Gag Epitope Map
Author Location	Gag
Epitope	`KAFSPEVIPMF`	Epitope Alignment
		Show epitope variants
Epitope Name	KF11
Subtype	B, C
Species (MHC/HLA)	human(B57:01, B57:03)
Immunogen	HIV-1 infection
Country	India, South Africa, United Kingdom, United States
Experimental methods	CD8 T-cell Elispot - IFNγ, Chromium-release assay, Flow cytometric T-cell cytokine assay, Proliferation
Keywords	subtype comparisons, escape, TCR usage, mutation acquisition

Notes

To study the contributions of HLA alleles and TCRs to the prevention of viral escape, HLAs B*5701 and B*5703 that differ at only 2 residues were followed with epitope KF11 and variants. B*5701-KF11 allowed fewer viral mutations along with a narrow TCR repertoire while B*5703-KF11 had a greater repertoire of TCR but also greater numbers of escape mutations. Therefore extensive TCR diversity is not a prerequisite to the prevention of viral mutations. More heterogeneous TCR-beta chains were also seen in the HLA-B*5703-KF11 situation and greater numbers of KF11 variants arose especially in clade C-infected subjects.
For both Clades B and C HIV-1, this epitope KAFSPEVIPMF (wt) i.e. KF11 spawned variants KgFSPEVIPMF (A2G), KnFSPEVIPMF (A2N), KsFSPEVIPMF (A2S), KsFnPEVIPMF (A2S-S4N) and KsFSPEiIPMF (A2S-V7I). In addition, Clade C also allowed variants KgFnPEVIPMF (A2G-S4(N/K)) and KgFkPEVIPMF (A2G-S4(N/K). Overall, HLA-B*5703 subjects showed more of these sequence variants than HLA-B*5701 possessing subjects did.

References

Yu2007a Xu G Yu, Mathias Lichterfeld, Senica Chetty, Katie L. Williams, Stanley K. Mui, Toshiyuki Miura, Nicole Frahm, Margaret E. Feeney, Yanhua Tang, Florencia Pereyra, Montiago X. Labute, Katja Pfafferott, Alisdair Leslie, Hayley Crawford, Rachel Allgaier, William Hildebrand, Richard Kaslow, Christian Brander, Todd M. Allen, Eric S. Rosenberg, Photini Kiepiela, Madhu Vajpayee, Paul A. Goepfert, Marcus Altfeld, Philip J. R. Goulder, and Bruce D Walker. Mutually Exclusive T-Cell Receptor Induction and Differential Susceptibility to Human Immunodeficiency Virus Type 1 Mutational Escape Associated with a Two-Amino-Acid Difference between HLA Class I Subtypes. J. Virol., 81(4):1619-1631, Feb 2007. PubMed ID: 17121793. Show all entries for this paper.