Search CTL/CD8+ T-Cell Epitope Database

Found 10 matching records:

Displaying record number 55270

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HXB2 Location	Gag(296-304) p24(164-172) DNA(1675..1701)	Gag Epitope Map
Author Location	p24(164-172)
Epitope	`YVDRFYKTL`	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human(A*02)
Immunogen	HIV-1 infection
Country	China
Experimental methods	CD8 T-cell Elispot - IFNγ, Intracellular cytokine staining, Other
Keywords	assay or method development, optimal epitope

Notes

HLA-A11-restricted CTL epitopes in 2 male Han Chinese subjects (one ART naive and one receiving HAART) were determined. A novel epitope was discovered and 5 other putative epitopes confirmed using 425 overlapping peptides spanning the entire HIV-1 clade B proteome. By employing intracellular cytokine staining, the authors developed a new method of mapping optimal epitopes and defining their HLA restriction efficiently. In spite of its being the most common HLA type in China, A*11-restricted epitopes were recognized less frequently than other epitopes, and A*11-restricted responses formed a mean of only 13% of the responses against HIV.
This epitope, YVDRFYKTL, was detected within overlapping peptides SILDIRQGPKEPFRDYV and GPKEPFRDYVDRFYKTLR.

References

Wang2007 Shaoyang Wang, Yongtao Sun, Song Zhai, Yan Zhuang, Shuguang Zhao, Whenzhen Kang, Xinhong Li, Dedong Huang, Xu G. Yu, Bruce D. Walker, and Marcus A. Altfeld. Identification of HLA-A11-Restricted HIV-1-Specific Cytotoxic T-Lymphocyte Epitopes in China. Curr. HIV Res., 5(1):119-128, Jan 2007. PubMed ID: 17266563. Show all entries for this paper.

Displaying record number 52525

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HXB2 Location	Gag(296-304) p24(164-172) DNA(1675..1701)	Gag Epitope Map
Author Location	p24(164-172)
Epitope	`YVDRFYKTL`	Epitope Alignment
Species (MHC/HLA)	human(A*02:07)
Immunogen	HIV-1 infection
Experimental methods
Keywords	optimal epitope, A-list

References

Llano2019 Anuska Llano, Samandhy Cedeño, Sandra Silva Arrieta, and Christian Brander. The 2019 Optimal HIV CTL Epitopes Update: Growing Diversity in Epitope Length and HLA restriction. 2019. Show all entries for this paper.

Displaying record number 61783

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HXB2 Location	Gag(296-304) p24(164-172) DNA(1675..1701)	Gag Epitope Map
Author Location
Epitope	`YVDRFYKTL`	Epitope Alignment
Epitope Name	YL9
Subtype	B
Species (MHC/HLA)	human(A*02:07)
Immunogen	HIV-1 infection, vaccine
Country	Japan
Experimental methods	CD8 T-cell Elispot - IFNγ, CTL suppression of replication, Intracellular cytokine staining
Keywords	vaccine-induced immune responses, variant cross-reactivity

Notes

Specific T cells - from 120 ART-naive, infected Japanese subjects previously exposed to the tHIVconsvX vaccine - that have the ability to suppress HIV-1 replication were identified as recognizing 17 optimal Gag epitopes. 9/17 eptiopes were associated with lower pVL and higher CD4 count, of which 5 remain associated with positive clinical outcome in 221 HIV-1 infected subjects and cross-recognized major HIV-1 variants. 2/5 epitopes were novel. Candidate tHIVconsvX vaccine is composed of 6 conserved Gag and Pol regions.
A*0207-restricted epitope YVDRFYKTL was found in 15-mer peptide pair C080/081. 3 HLA-carrying and 13 non-HLA-A*0207-carrying responders to this epitope were detected by EliSpot out of 50 subjects.

References

Murakoshi2018 Hayato Murakoshi, Chengcheng Zou, Nozomi Kuse, Tomohiro Akahoshi, Takayuki Chikata, Hiroyuki Gatanaga, Shinichi Oka, Tomáš Hanke, and Masafumi Takiguchi. CD8+ T Cells Specific for Conserved, Cross-Reactive Gag Epitopes with Strong Ability to Suppress HIV-1 Replication. Retrovirology, 15(1):46, 3 Jul 2018. PubMed ID: 29970102. Show all entries for this paper.

Displaying record number 52330

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HXB2 Location	Gag(296-304) p24(164-172) DNA(1675..1701)	Gag Epitope Map
Author Location	Gag(296-304)
Epitope	`YVDRFYKTL`	Epitope Alignment
		Show epitope variants
Subtype	B
Species (MHC/HLA)	human(A*02:07)
Immunogen	HIV-1 infection
Patient MHC/HLA	A*02:07
Experimental methods
Keywords	subtype comparisons

Notes

Cross-reactive responses were found in PBMC isolated from individuals infected with either B or CRF01_AE clade viruses, as determined by Elispot assays of target cells expressing recombinant vaccinia viruses expressing HIV-1 gag, env, nef and pol from many clades.
The Thai subject VAIP-4 demonstrated broad CTL cross-reactivity towards gag constructs derived from subtypes A, B, C, D, F, G, H, and CRF-01_AE. Sequence alignments of this epitope showed conservation for clades B and D, and Y->F substitutions at position 6 for subtypes A, C, CDR01-AE, F, G, and H. YVDRFYKTL and the variant epitope YVDRFFKTL are recognized equally well.

References

Currier2002b Jeffrey R Currier, Mark deSouza, Penprapa Chanbancherd, Wendy Bernstein, Deborah L Birx, and Josephine H Cox. Comprehensive Screening for Human Immunodeficiency Virus Type 1 Subtype-Specific CD8 Cytotoxic T Lymphocytes and Definition of Degenerate Epitopes Restricted by HLA-A0207 and -Cw0304 Alleles. J. Virol., 76(10):4971-4986, May 2002. PubMed ID: 11967314. Show all entries for this paper.

Displaying record number 54229

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HXB2 Location	Gag(296-304) p24(164-172) DNA(1675..1701)	Gag Epitope Map
Author Location	p24
Epitope	`YVDRFYKTL`	Epitope Alignment
Epitope Name	A2-YL9(p24)
Species (MHC/HLA)	human(A2)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	rate of progression, immunodominance, early-expressed proteins

Notes

Immunodominant HIV epitopes in early, primary infection of CTLs were identified and the relative contributions of immune responses restricted to individual HLA class I molecules were studied.
This epitope-HLA combination was recognized. The most frequently recognized epitopes also elicited the greatest CTL response.
HIV-1 Epitope sequence variability was not correlated with immunodominant versus immune sub-dominant responses.
HLA-B*57 and HLA-B*27 restricted responses were the strongest in these early and acute infections - due to dominant responses to a single epitope in each case (TW10 for B*57 and KK10 for B*27).
In the presence of HLA-B*57 and HLA-B*27 restricted responses, the relative contributions of other restricting HLA dropped greatly.

References

Altfeld2006 Marcus Altfeld, Elizabeth T. Kalife, Ying Qi, Hendrik Streeck, Mathias Lichterfeld, Mary N. Johnston, Nicole Burgett, Martha E. Swartz, Amy Yang, Galit Alter, Xu G. Yu, Angela Meier, Juergen K. Rockstroh, Todd M. Allen, Heiko Jessen, Eric S. Rosenberg, Mary Carrington, and Bruce D. Walker. HLA Alleles Associated with Delayed Progression to AIDS Contribute Strongly to the Initial CD8+ T Cell Response against HIV-1. PLoS Med., 3(10):e403, Oct 2006. PubMed ID: 17076553. Show all entries for this paper.

Displaying record number 1464

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HXB2 Location	Gag(296-304) p24(164-172) DNA(1675..1701)	Gag Epitope Map
Author Location	p24(164-172)
Epitope	`YVDRFYKTL`	Epitope Alignment
Species (MHC/HLA)	human(B70)
Immunogen	HIV-1 infection
Experimental methods
Keywords

Notes

One of the 51 HIV-1 epitopes selected by Ferrari et al. as good candidate CTL epitopes for vaccines by virtue of being conserved and presented by common HLA alleles.

References

Ferrari2000 G. Ferrari, D. D. Kostyu, J. Cox, D. V. Dawson, J. Flores, K. J. Weinhold, and S. Osmanov. Identification of highly conserved and broadly cross-reactive HIV type 1 cytotoxic T lymphocyte epitopes as candidate immunogens for inclusion in Mycobacterium bovis BCG-vectored HIV vaccines. AIDS Res. Hum. Retroviruses, 16(14):1433-43, 20 Sep 2000. PubMed ID: 11018863. Show all entries for this paper.

Displaying record number 61784

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HXB2 Location	Gag(296-304) p24(164-172) DNA(1675..1701)	Gag Epitope Map
Author Location
Epitope	`YVDRFYKTL`	Epitope Alignment
Epitope Name	YL9
Subtype	B
Species (MHC/HLA)	human(C*03)
Immunogen	HIV-1 infection, vaccine
Country	Japan
Experimental methods	CD8 T-cell Elispot - IFNγ, CTL suppression of replication, Intracellular cytokine staining
Keywords	vaccine-induced immune responses, variant cross-reactivity

Notes

Specific T cells - from 120 ART-naive, infected Japanese subjects previously exposed to the tHIVconsvX vaccine - that have the ability to suppress HIV-1 replication were identified as recognizing 17 optimal Gag epitopes. 9/17 eptiopes were associated with lower pVL and higher CD4 count, of which 5 remain associated with positive clinical outcome in 221 HIV-1 infected subjects and cross-recognized major HIV-1 variants. 2/5 epitopes were novel. Candidate tHIVconsvX vaccine is composed of 6 conserved Gag and Pol regions.
Cw*03-restricted epitope YVDRFYKTL was found in 15-mer peptide pair C080/081. 9 HLA-carrying and 7 non-HLA-Cw*03-carrying responders to this epitope were detected by EliSpot out of 50 subjects.

References

Displaying record number 61506

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HXB2 Location	Gag(296-304) p24(164-172) DNA(1675..1701)	Gag Epitope Map
Author Location	Gag(296-304)
Epitope	`YVDRFYKTL`	Epitope Alignment
Epitope Name	GagYL9
Subtype	B
Species (MHC/HLA)	human(C*03:04)
Immunogen	HIV-1 infection
Country	Japan
Experimental methods	CD8 T-cell Elispot - IFNγ, Intracellular cytokine staining, Other
Keywords	optimal epitope, chronic infection

Notes

CTL controlling HIV-1 and variants were identified from 401 subtype B chronically-infected, treatment-naive Japanese patients. 7 novel epitopes were identified (paper reports 9, correction made via author communication). 9/13 CTL-specific epitopes were conserved among the above subjects and 3/13 were cross-recognized by CTL. 5/13 epitopes were B*5201, 3/13 were B*6701-restricted, both HLA were found to play a predominant role in control of HIV-1. This is the first study to identify HIV-1-specific T cells clinically controlling HIV-1 in a population in whom protective HLA-B*57 and HLA-B*27 are very rare.
Breadth of response of all 13 epitope-specific CTL had synergistic effects on control, directly correlating to CD4 cell count and inversely to plasma viral load (pVL).
HLA-Cw*0304-restricted GagYL9, YVDRFYKTL, had 2/393 responding subjects and did not significantly correlate with pVL or CD4 count.

References

Murakoshi2015 Hayato Murakoshi, Tomohiro Akahoshi, Madoka Koyanagi, Takayuki Chikata, Takuya Naruto, Rie Maruyama, Yoshiko Tamura, Naoki Ishizuka, Hiroyuki Gatanaga, Shinichi Oka, and Masafumi Takiguchi. Clinical Control of HIV-1 by Cytotoxic T Cells Specific for Multiple Conserved Epitopes. J. Virol., 89(10):5330-5339, May 2015. PubMed ID: 25741000. Show all entries for this paper.

Displaying record number 62818

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HXB2 Location	Gag(296-304) p24(164-172) DNA(1675..1701)	Gag Epitope Map
Author Location	p24(296-304)
Epitope	`YVDRFYKTL`	Epitope Alignment
		Show epitope variants
Subtype	B
Species (MHC/HLA)	human
Immunogen	engineered
Experimental methods	Other, Relative replication capacity assay
Keywords	assay or method development, protective allele

Notes

NL4-3 Gag polyprotein random mutagenesis, high-throughput fitness profiling and in silico prediction of MHC-peptide binding affinity revealed that mutations in CTL epitopes presented by protective MHC-I alleles, vs. non-protective alleles, showed greater loss of experimental replicative capacity and lower levels of predicted reduction in MHC-I binding affinity. Together, these two variables could predict protectiveness of MHC-I alleles in a linear regression model. Analyses of intraperson viral evolution in 4 LTNP vs. 4 paired progressor subjects further supported paper conclusions.
After mutagenesis and passaging through human T cells, 63 single nucleotide mutations were observed in epitope YVDRFYKTL (known to bind to A0207) & variant YVDRFfKTL (known to bind to B1503, Cw0303 & Cw0304) with a log transformation of mean fitness score, relative to NL4-3, of -0.6969.

References

Du2017 Yushen Du, Tian-Hao Zhang, Lei Dai, Xiaojuan Zheng, Aleksandr M. Gorin, John Oishi, Ting-Ting Wu, Janice M. Yoshizawa, Xinmin Li, Otto O. Yang, Otoniel Martinez-Maza, Roger Detels, and Ren Sun. Effects of Mutations on Replicative Fitness and Major Histocompatibility Complex Class I Binding Affinity Are Among the Determinants Underlying Cytotoxic-T-Lymphocyte Escape of HIV-1 Gag Epitopes. mBio, 8(6), 28 Nov 2017. PubMed ID: 29184023. Show all entries for this paper.

Displaying record number 55899

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HXB2 Location	Gag(296-304) p24(164-172) DNA(1675..1701)	Gag Epitope Map
Author Location	p24
Epitope	`YVDRFYKTL`	Epitope Alignment
		Show epitope variants
Epitope Name	YL9(p24)
Subtype	B
Species (MHC/HLA)	human
Immunogen	HIV-1 infection
Country	China
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	variant cross-reactivity, chronic infection

Notes

49 ART-naive chronically HIV-1 infected Chinese subjects' CTLs were tested with 413 OLPs in IFN-γ assays, determining optimal epitopes specific for Chinese Clade B HIV-1 sequences.
An inverse correlation was found between CTL response and viral load.
Targeted peptides have lower entropy than peptides that are not CTL targeted. This correlation is independent of HLA-restriction.
Previously described epitope YVDRFYKTL elicited an immune response as part of peptide YVDRFYKTLRAEQASQEV as well as peptides GPKEPFRDYVDRFYKTLR and YYVDRFYKTLRAEQASQEV. This epitope differs from the previously described HLA-A2 and B15-restricted epitope, YVDRFFKTL, at 1 residue, YVDRFyKTL.
18 of the 55 HLA-A2 carriers responded to a YVDRFyKTL-containing peptide with average magnitude of CTL response of 334 SFC/million PBMC; 9 of the 21 HLA-B15 carriers responded to a YVDRFyKTL-containing peptide with average magnitude of CTL response of 542 SFC/million PBMC (author communication and Fig.1).

References

Zhai2008 Song Zhai, Yan Zhuang, Yang Song, Shu Li, Dedong Huang, Wenzhen Kang, Xinhong Li, Qi Liao, Yanhou Liu, Zhongfang Zhao, Yichen Lu, and Yongtao Sun. HIV-1-Specific Cytotoxic T Lymphocyte (CTL) Responses Against Immunodominant Optimal Epitopes Slow the Progression of AIDS in China. Curr. HIV Res., 6(4):335-350, Jun 2008. PubMed ID: 18691032. Show all entries for this paper.