Search CTL/CD8+ T-Cell Epitope Database

Found 2 matching records:

Displaying record number 55844

Download this epitope record as JSON.

HXB2 Location	gp160(183-191) gp120(183-191) DNA(6771..6797)	gp160 Epitope Map
Author Location	Env
Epitope	YSENSSEYY	Epitope Alignment
Subtype	C
Species (MHC/HLA)	human(A*01)
Immunogen	vaccine
Country	Switzerland
Experimental methods	CD8 T-cell Elispot - IFNγ, Other, Proliferation
Keywords	vaccine-induced immune responses, vaccine antigen design

Vaccine Details

Notes

• A poxvirus based vaccine against HIV-1 subtype C was characterized and found to be immunogenic. DNA prime, NYVAC boost vaccine containing Env, Gag, Pol, and Nef as a polypeptide immunogen elicited T-cell responses in 90% vaccinees.
• Vaccine induced T-cell response was of a high magnitude and polyfunctional with respect to CD4 and CD8 cell activation. Each responding vaccinee elicited broad responses to multiple epitopes, especially in Env, that persisted.
• An optimal CTL Env epitope YSENSSEYY, not previously described elicited immune response.
• 9 novel Env epitopes, both CTL and helper, were found to be targeted after vaccination in subjects.

References

Harari2008 Alexandre Harari, Pierre-Alexandre Bart, Wolfgang Stöhr, Gonzalo Tapia, Miguel Garcia, Emmanuelle Medjitna-Rais, Séverine Burnet, Cristina Cellerai, Otto Erlwein, Tristan Barber, Christiane Moog, Peter Liljestrom, Ralf Wagner, Hans Wolf, Jean-Pierre Kraehenbuhl, Mariano Esteban, Jonathan Heeney, Marie-Joelle Frachette, James Tartaglia, Sheena McCormack, Abdel Babiker, Jonathan Weber, and Giuseppe Pantaleo. An HIV-1 Clade C DNA Prime, NYVAC Boost Vaccine Regimen Induces Reliable, Polyfunctional, and Long-Lasting T Cell Responses. J. Exp. Med., 205(1):63-77, 21 Jan 2008. PubMed ID: 18195071. Show all entries for this paper.

Displaying record number 55282

Download this epitope record as JSON.

HXB2 Location	gp160(183-191) gp120(183-191) DNA(6771..6797)	gp160 Epitope Map
Author Location	Env(173-177)
Epitope	YSENSSEYY	Epitope Alignment
Subtype	B, C
Species (MHC/HLA)	human
Immunogen	vaccine
Country	Switzerland, United States
Experimental methods	Flow cytometric T-cell cytokine assay, Other, Tetramer binding
Keywords	vaccine-induced immune responses, characterizing CD8+ T cells

Vaccine Details

Notes

• Vaccines against vaccinia (MVA or Dryvax) or HIV-1 clade C (NYVAC, a recombinant vaccinia virus) induce a similar polyfunctional CTL profile, secreting IFN-gamma, IL-2, MIP-1beta and TNF-alpha, as well as being CD107a+. CD45RO-CD27intermediate phenotype-polyfunctional CTLs secrete more IFN-gamma than monofunctional CTLs.

References

Precopio2007 Melissa L. Precopio, Michael R. Betts, Janie Parrino, David A. Price, Emma Gostick, David R. Ambrozak, Tedi E. Asher, Daniel C. Douek, Alexandre Harari, Giuseppe Pantaleo, Robert Bailer, Barney S. Graham, Mario Roederer, and Richard A. Koup. Immunization with Vaccinia Virus Induces Polyfunctional and Phenotypically Distinctive CD8+ T Cell Responses. J. Exp. Med., 204(6):1405-1416, 11 Jun 2007. PubMed ID: 17535971. Show all entries for this paper.