Search CTL/CD8+ T-Cell Epitope Database

Found 8 matching records:

Displaying record number 52560

HXB2 Location	Vif(79-87) DNA(5275..5301)	Vif Epitope Map
Author Location	Vif(79-87)
Epitope	`WHLGHGVSI`	Epitope Alignment
Species (MHC/HLA)	human(B*1510)
Immunogen	HIV-1 infection
Experimental methods
Keywords	optimal epitope

Notes

An A-list optimal epitope noted by C. Brander to be restricted by B*1510.

References

Llano2019 Anuska Llano, Samandhy Cedeño, Sandra Silva Arrieta, and Christian Brander. The 2019 Optimal HIV CTL Epitopes Update: Growing Diversity in Epitope Length and HLA restriction. 2019. Show all entries for this paper.

Displaying record number 53271

HXB2 Location	Vif(79-87) DNA(5275..5301)	Vif Epitope Map
Author Location	Nef( C consensus)
Epitope	`WHLGHGVSI`	Epitope Alignment
Epitope Name	WI9
Subtype	C
Species (MHC/HLA)	human(B*1510)
Immunogen	HIV-1 infection
Patient MHC/HLA	A2601, A7401, B0801, B1510, Cw0202, Cw0801
Country	South Africa
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	assay or method development, characterizing CD8+ T cells

Notes

HLA class I restricted CD8+ T-cell responses against HIV-1 were analyzed in African patients. Significantly more responses were shown to be HLA-B restricted. Viral load, CD4 count, and thus rate of disease progression were also associated with HLA-B alleles. In addition, the selection pressure imposed on HIV-1 by HLA-B alleles was shown to be substantially greater than by other alleles.
This epitope was one of two used to illustrate how specific epitopes were characterized with regard to defining the optimal epitope and the HLA restricting element. HLA allelic associations in the population with peptide recognition was generally high predictive of the epitope within the 15 mer.

References

Kiepiela2004 Photini Kiepiela, Alasdair J. Leslie, Isobella Honeyborne, Danni Ramduth, Christina Thobakgale, Senica Chetty, Prinisha Rathnavalu, Corey Moore, Katja J. Pfafferott, Louise Hilton, Peter Zimbwa, Sarah Moore, Todd Allen, Christian Brander, Marylyn M. Addo, Marcus Altfeld, Ian James, Simon Mallal, Michael Bunce, Linda D. Barber, James Szinger, Cheryl Day, Paul Klenerman, James Mullins, Bette Korber, Hoosen M. Coovadia, Bruce D. Walker, and Philip J. R. Goulder. Dominant Influence of HLA-B in Mediating the Potential Co-Evolution of HIV and HLA. Nature, 432(7018):769-775, 9 Dec 2004. LA-UR 04-5920. PubMed ID: 15592417. Show all entries for this paper.

Displaying record number 53294

HXB2 Location	Vif(79-87) DNA(5275..5301)	Vif Epitope Map
Author Location	( C consensus)
Epitope	`WHLGHGVSI`	Epitope Alignment
Subtype	C
Species (MHC/HLA)	human(B*1510)
Immunogen	HIV-1 infection
Country	South Africa
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	characterizing CD8+ T cells

Notes

HLA class I restricted CD8+ T-cell responses against HIV-1 were analyzed in African patients. Significantly more responses were shown to be HLA-B restricted. Viral load, CD4 count, and thus rate of disease progression were also associated with HLA-B alleles. In addition, the selection pressure imposed on HIV-1 by HLA-B alleles was shown to be substantially greater than by other alleles.
This epitope was suggested to be the epitope within a longer reactive peptide based on correspondence with a known epitope in the HIV database. Also, a significantly higher frequency of people in the Durban cohort who reacted with the peptide had this HLA type.

References

Displaying record number 53953

HXB2 Location	Vif(79-87) DNA(5275..5301)	Vif Epitope Map
Author Location	( C consensus)
Epitope	`WHLGHGVSI`	Epitope Alignment
Subtype	C
Species (MHC/HLA)	human(B*1510)
Immunogen	HIV-1 infection
Country	South Africa
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	epitope processing, rate of progression, optimal epitope

Notes

A comprehensive analysis of 160 class I T cell responses in 578 individuals from KwaZulu-Natal, South Africa was performed. Gag-specific responses were associated with lowering viremia, while Env, accessory and regulatory protein-specific responses were associated with higher viremia.
Mutational patterns in the V7 residue of WHLGHGVSI are associated with the presence of the HLA presenting molecule in the host. Mutation of a residue outside of the optimized epitope is also associated with the HLA.

References

Kiepiela2007 Photini Kiepiela, Kholiswa Ngumbela, Christina Thobakgale, Dhanwanthie Ramduth, Isobella Honeyborne, Eshia Moodley, Shabashini Reddy, Chantal de Pierres, Zenele Mncube, Nompumelelo Mkhwanazi, Karen Bishop, Mary van der Stok, Kriebashnie Nair, Nasreen Khan, Hayley Crawford, Rebecca Payne, Alasdair Leslie, Julia Prado, Andrew Prendergast, John Frater, Noel McCarthy, Christian Brander, Gerald H. Learn, David Nickle, Christine Rousseau, Hoosen Coovadia, James I. Mullins, David Heckerman, Bruce D. Walker, and Philip Goulder. CD8+ T-Cell Responses to Different HIV Proteins Have Discordant Associations with Viral Load. Nat. Med., 13(1):46-53, Jan 2007. PubMed ID: 17173051. Show all entries for this paper.

Displaying record number 54186

HXB2 Location	Vif(79-87) DNA(5275..5301)	Vif Epitope Map
Author Location	Vif(79-87)
Epitope	`WHLGHGVSI`	Epitope Alignment
Subtype	C
Species (MHC/HLA)	human(B*1510)
Immunogen	HIV-1 infection
Experimental methods	Other
Keywords	epitope processing, HLA associated polymorphism

Notes

Publicly available HIV-1 subtype C sequences from South Africa and Botswana were analyzed, and 94 HLA-associated amino acid polymorphisms were found. Many polymorphisms were associated with multiple HLA allele classes. 12% associations were negative: association of virus expression of the consensus amino acid with a specific HLA allele. HLA-B alleles accounted for 50% of associations. 19 HLA-associated polymorphisms were embedded in previously defined epitopes.
WHLGHGVSI was a previously defined B*1510 presented epitope that was associated with a polymorphism, d|WHLGHGVSI,in the first position before that epitope. This epitope was embedded in a previoulsy identified CTL immunoreactive region.

References

Boutwell2007 Christian L. Boutwell and M. Essex. Identification of HLA Class I-Associated Amino Acid Polymorphisms in the HIV-1C Proteome. AIDS Res. Hum. Retroviruses, 23(1):165-174, Jan 2007. PubMed ID: 17263647. Show all entries for this paper.

Displaying record number 56240

HXB2 Location	Vif(79-87) DNA(5275..5301)	Vif Epitope Map
Author Location
Epitope	`WHLGHGVSI`	Epitope Alignment
Subtype	C
Species (MHC/HLA)	human(B*1510)
Immunogen	HIV-1 infection
Patient MHC/HLA	A2301, A2902, B1510, B4501, Cw0602, Cw1601
Country	South Africa
Experimental methods	CD8 T-cell Elispot - IFNγ, Chromium-release assay
Keywords	rate of progression, optimal epitope

Notes

47 HIV-1 subtype C infected patients were followed longitudinally to determine whether there was a relation between magnitude and breadth of CTL responses in primary, acute infection (measured by IFN-gamma ELISpot) and disease progression (measured as viral load at 12 months). CTL responses to Nef were dominant (targeting 6 epitopic regions), followed by responses to Gag, Pol, Rev, Vpr, Env, Vpu, Vif and finally Tat.
Known epitope, WHLGHGVSI, is HLA-B*1510-restricted. Response to a peptide containing this epitope was detected in an early controller 12 weeks post-infection.

References

Gray2009 Clive M. Gray, Mandla Mlotshwa, Catherine Riou, Tiyani Mathebula, Debra de Assis Rosa, Tumelo Mashishi, Cathal Seoighe, Nobubelo Ngandu, Francois van Loggerenberg, Lynn Morris, Koleka Mlisana, Carolyn Williamson, Salim Abdool Karim, and CAPRISA 002 Acute Infection Study Team. Human Immunodeficiency Virus-Specific gamma Interferon Enzyme-Linked Immunospot Assay Responses Targeting Specific Regions of the Proteome during Primary Subtype C Infection Are Poor Predictors of the Course of Viremia and Set Point. J. Virol., 83(1):470-478, Jan 2009. PubMed ID: 18945774. Show all entries for this paper.

Displaying record number 61038

HXB2 Location	Vif(79-87) DNA(5275..5301)	Vif Epitope Map
Author Location	Vif(79-87)
Epitope	`WHLGHGVSI`	Epitope Alignment
Subtype	C
Species (MHC/HLA)	human(B*1510)
Immunogen	HIV-1 infection
Patient MHC/HLA	CAP045: A2301, A2902, B1510, B4501, Cw1601, Cw1602
Country	South Africa
Experimental methods	CD8 T-cell Elispot - IFNγ, Other
Keywords	computational epitope prediction, escape, acute/early infection, mutation acquisition

Notes

17 patients with acute HIV-1 infection were followed to validate that (1) relative escape rate (2) location and identity of escape mutations and (3) epistatic effects on escape, could be predicted within a host by using a simple model based on population genetics and known fitness measures of HIV polyproteins. In general escape location was found most at residues with the lowest fitness cost and escape time correlates with early detection of mutants when T cell response was first detected.
Epitope WHLGHGVSI had a fitness cost ΔE of 4.672 and T cell response was first detected to it at 21d, escape time was 408.47 days.

References

Barton2016 John P. Barton, Nilu Goonetilleke, Thomas C. Butler, Bruce D. Walker, Andrew J. McMichael, and Arup K. Chakraborty. Relative Rate and Location of Intra-Host HIV Evolution to Evade Cellular Immunity Are Predictable. Nat. Commun., 7:11660, 23 May 2016. PubMed ID: 27212475. Show all entries for this paper.

Displaying record number 61045

HXB2 Location	Vif(79-87) DNA(5275..5301)	Vif Epitope Map
Author Location	Vif(79-87)
Epitope	`WHLGHGVSI`	Epitope Alignment
Subtype	C
Species (MHC/HLA)	human(B*1510)
Immunogen	HIV-1 infection
Patient MHC/HLA	CAP210: A6802, B1510, Cw*0304
Country	South Africa
Experimental methods	CD8 T-cell Elispot - IFNγ, Other
Keywords	computational epitope prediction, escape, acute/early infection, mutation acquisition

Notes

17 patients with acute HIV-1 infection were followed to validate that (1) relative escape rate (2) location and identity of escape mutations and (3) epistatic effects on escape, could be predicted within a host by using a simple model based on population genetics and known fitness measures of HIV polyproteins. In general escape location was found most at residues with the lowest fitness cost and escape time correlates with early detection of mutants when T cell response was first detected.
Epitope WHLGHGVSI had a fitness cost ΔE of 2.828 and T cell response was first detected to it at 22d, escape time was 127.37 days.