Search CTL/CD8+ T-Cell Epitope Database

Found 3 matching records:

Displaying record number 52672

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HXB2 Location	gp160(36-44) gp120(36-44) DNA(6330..6356)	gp160 Epitope Map
Author Location	Env(35-)
Epitope	VTVYYGVPV	Epitope Alignment
Epitope Name	Env35
Species (MHC/HLA)	human(A2)
Immunogen	HIV-1 infection, vaccine
Experimental methods	CD4 T-cell Elispot - IFNγ, Chromium-release assay, Flow cytometric T-cell cytokine assay
Keywords	binding affinity, subtype comparisons, computational epitope prediction

Vaccine Details

Notes

• HLA-A2-restricted HIV-1 CTL epitopes were computationally predicted. Binding affinities for HLA-A*0204, immunogenicity in HLA-A*0201 transgenic mice, and responses to the peptides in 17 HIV-1 infected patients were tested. 31 novel conserved A2 epitopes were detected. An average of 4 epitopes were recognized per patient.
• This peptide was a good A2 binder, and induced a CD8+ T-cell IFN gamma response in 1/6 mice. Responses to the peptide were not detected in 17 HIV+ HLA-A2 subjects.

References

Corbet2003 Sylvie Corbet, Henrik Vedel Nielsen, Lasse Vinner, Sanne Lauemoller, Dominic Therrien, Sheila Tang, Gitte Kronborg, Lars Mathiesen, Paul Chaplin, Søren Brunak, Søren Buus, and Anders Fomsgaard. Optimization and Immune Recognition of Multiple Novel Conserved HLA-A2, Human Immunodeficiency Virus Type 1-Specific CTL Epitopes. J. Gen. Virol., 84(Pt 9):2409-2421, Sep 2003. PubMed ID: 12917462. Show all entries for this paper.

Displaying record number 55123

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HXB2 Location	gp160(36-44) gp120(36-44) DNA(6330..6356)	gp160 Epitope Map
Author Location	gp120(36-44)
Epitope	VTVYYGVPV	Epitope Alignment
Species (MHC/HLA)	human(A2)
Immunogen
Experimental methods
Keywords	subtype comparisons, viral fitness and reversion

Notes

• Determined the frequencies of known CD8+ and CD4+ Env epitope sequences among Brazilian HIV sequences of various subtypes.
• Present in 51/51 Brazilian HIV sequences.

References

Queiroz2007 Artur Trancoso Lopo de Queiroz, Luciane Amorim Santos, Domingos Ramon Moreau, Tulio de Oliveira, David I. Watkins, Bernardo Galvão-Castro, and Luiz Carlos Junior Alcantara. Identification and Characterization of Previously Described Epitopes in HIV-1 Subtypes B, C, F and BF in Brazil. Braz. J. Infect. Dis., 11(1):27-30, Feb 2007. PubMed ID: 17625722. Show all entries for this paper.

Displaying record number 55378

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HXB2 Location	gp160(36-44) gp120(36-44) DNA(6330..6356)	gp160 Epitope Map
Author Location
Epitope	VTVYYGVPV	Epitope Alignment
Epitope Name	Env 35
Species (MHC/HLA)	human(A2)
Immunogen	HIV-1 infection
Country	Denmark
Experimental methods	CD8 T-cell Elispot - IFNγ, Chromium-release assay, Flow cytometric T-cell cytokine assay, Intracellular cytokine staining
Keywords

Notes

• 11 HLA-A2+ (A*0201, A*0220, A*0234 or A*0236), patients' HIV were sequenced. Of 28 epitopes studied, most were conserved and elicited a subdominant CTL response. 9 epitopes in 7 proteins showed T-cell cross reaction. These epitopes were optimized to induce more immunogenic but cross-reacting responses.
• Epitopes in Gag, Pol, Env, Nef were more conserved than epitopes in shorter proteins Vpu, Vif, Vpr and Rev. Most natural epitopes were untargeted or targeted by 1 or 2 patients.
• Previously defined Env 35 VTVYYGVPV epitope was found in all 11 patients but none had CTL immune responses to it.

References

Thorn2007 Mette Thorn, Sheila Tang, Dominic Therrien, Henrik Kløverpris, Lasse Vinner, Gitte Kronborg, Jan Gerstoft, Sylvie Corbet, and Anders Fomsgaard. Sequence Conservation of Subdominant HLA-A2-Binding CTL Epitopes in HIV-1 Clinical Isolates and CD8+ T-Lymphocyte Cross-Recognition May Explain the Immune Reaction in Infected Individuals. APMIS, 115(6):757-768, Jun 2007. PubMed ID: 17550385. Show all entries for this paper.