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Displaying record number 52345

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HXB2 Location	Nef(180-189) DNA(9334..9363)	Nef Epitope Map
Author Location	Nef(180-189 LAI)
Epitope	`VLMWQFDSRL`	Epitope Alignment
Subtype	B
Species (MHC/HLA)	transgenic mouse(A*02:01)
Immunogen	vaccine
Experimental methods
Keywords	binding affinity, vaccine-induced immune responses

Vaccine Details

Vaccine type	peptide
Vaccine strain	natural variants
Vaccine component	Nef
Adjuvant	Complete Freund's Adjuvant (CFA)

Notes

Ten naturally occurring variants of this epitope were tested for their affinity to HLA-A*0201 and for their ability to induce gamma-IFN and cytotoxic functions through vaccination of HLA-A*0201 transgenic mice.
Only two variants could induce vaccine responses: VLMWQFDSRL, a high affinity binder, and VLQWRFDSRL a medium affinity binder to A*0201.
In vivo priming with Nef peptide VLMWQFDSRL induced cross-reactive CTL to 6/7 peptides tested (AlmwKfdsKl, vlmwKfdsrl, vlmwKfdsKl, vlQwRfdsKl, vlVwrfdTrl, and vlAwKLdsrl but not the LAI peptide vlEwrfdsrl)
In vivo priming with Nef peptide VLQWRFDTRL induced cross-reactive CTL to 3/6 variant Nef peptides (vlMwQfdsrl, vlqwrfdSrl and vlEwrfdsrl).

References

Boissonnas2002 Alexandre Boissonnas, Olivia Bonduelle, Ariane Antzack, Yu-Chun Lone, Cecile Gache, Patrice Debre, Brigitte Autran, and Behazine Combadiere. In Vivo Priming Of HIV-Specific CTLs Determines Selective Cross-Reactive Immune Responses Against Poorly Immunogenic HIV-Natural Variants. J. Immunol., 169(7):3694-3699, 1 Oct 2002. PubMed ID: 12244162. Show all entries for this paper.