Search CTL/CD8+ T-Cell Epitope Database

Found 7 matching records:

Displaying record number 53962

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HXB2 Location	Gag(143-152) p24(11-20) DNA(1216..1245)	Gag Epitope Map
Author Location	( C consensus)
Epitope	`VHQAISPRTL`	Epitope Alignment
Subtype	C
Species (MHC/HLA)	human(B*15:10)
Immunogen	HIV-1 infection
Country	South Africa
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	rate of progression, optimal epitope

Notes

A comprehensive analysis of 160 class I T cell responses in 578 individuals from KwaZulu-Natal, South Africa was performed. Gag-specific responses were associated with lowering viremia, while Env, accessory and regulatory protein-specific responses were associated with higher viremia.
VHQAISPRTL is an optimal epitope.

References

Kiepiela2007 Photini Kiepiela, Kholiswa Ngumbela, Christina Thobakgale, Dhanwanthie Ramduth, Isobella Honeyborne, Eshia Moodley, Shabashini Reddy, Chantal de Pierres, Zenele Mncube, Nompumelelo Mkhwanazi, Karen Bishop, Mary van der Stok, Kriebashnie Nair, Nasreen Khan, Hayley Crawford, Rebecca Payne, Alasdair Leslie, Julia Prado, Andrew Prendergast, John Frater, Noel McCarthy, Christian Brander, Gerald H. Learn, David Nickle, Christine Rousseau, Hoosen Coovadia, James I. Mullins, David Heckerman, Bruce D. Walker, and Philip Goulder. CD8+ T-Cell Responses to Different HIV Proteins Have Discordant Associations with Viral Load. Nat. Med., 13(1):46-53, Jan 2007. PubMed ID: 17173051. Show all entries for this paper.

Displaying record number 56467

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HXB2 Location	Gag(143-152) p24(11-20) DNA(1216..1245)	Gag Epitope Map
Author Location	p24
Epitope	`VHQAISPRTL`	Epitope Alignment
Subtype	C
Species (MHC/HLA)	human(B*15:10)
Immunogen	HIV-1 infection
Country	South Africa
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	viral fitness and reversion, HLA associated polymorphism

Notes

HLA-associated polymorphisms were studied in a cohort of 710 chronic subtype C patients from South Africa. 84 HLA Class I-associated polymorphisms were identified, 73% of them with HLA-B. The viral set points associated with HLA allele correlated with the number of reversions associated with that allele, (HLA-B in Gag, HLA-C in Pol).
VHQAISPRTL is a previously described HLA-B*1510-restricted epitope (part of reacting peptide QNLQGQMVHQAISPRTLNAWV) that contains a B*1510-associated reversion at residue A (VHQaISPRTL).

References

Matthews2008 Philippa C. Matthews, Andrew Prendergast, Alasdair Leslie, Hayley Crawford, Rebecca Payne, Christine Rousseau, Morgane Rolland, Isobella Honeyborne, Jonathan Carlson, Carl Kadie, Christian Brander, Karen Bishop, Nonkululeko Mlotshwa, James I. Mullins, Hoosen Coovadia, Thumbi Ndung'u, Bruce D. Walker, David Heckerman, and Philip J. R. Goulder. Central Role of Reverting Mutations in HLA Associations with Human Immunodeficiency Virus Set Point. J. Virol., 82(17):8548-8559, Sep 2008. PubMed ID: 18596105. Show all entries for this paper.

Displaying record number 53293

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HXB2 Location	Gag(143-152) p24(11-20) DNA(1216..1245)	Gag Epitope Map
Author Location	( C consensus)
Epitope	`VHQAISPRTL`	Epitope Alignment
Subtype	C
Species (MHC/HLA)	human(B*15:10)
Immunogen	HIV-1 infection
Country	South Africa
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	characterizing CD8+ T cells

Notes

HLA class I restricted CD8+ T-cell responses against HIV-1 were analyzed in African patients. Significantly more responses were shown to be HLA-B restricted. Viral load, CD4 count, and thus rate of disease progression were also associated with HLA-B alleles. In addition, the selection pressure imposed on HIV-1 by HLA-B alleles was shown to be substantially greater than by other alleles.
This epitope was suggested to be the epitope within a longer reactive peptide based on correspondence with a known epitope in the HIV database. Also, a significantly higher frequency of people in the Durban cohort who reacted with the peptide had this HLA type.

References

Kiepiela2004 Photini Kiepiela, Alasdair J. Leslie, Isobella Honeyborne, Danni Ramduth, Christina Thobakgale, Senica Chetty, Prinisha Rathnavalu, Corey Moore, Katja J. Pfafferott, Louise Hilton, Peter Zimbwa, Sarah Moore, Todd Allen, Christian Brander, Marylyn M. Addo, Marcus Altfeld, Ian James, Simon Mallal, Michael Bunce, Linda D. Barber, James Szinger, Cheryl Day, Paul Klenerman, James Mullins, Bette Korber, Hoosen M. Coovadia, Bruce D. Walker, and Philip J. R. Goulder. Dominant Influence of HLA-B in Mediating the Potential Co-Evolution of HIV and HLA. Nature, 432(7018):769-775, 9 Dec 2004. LA-UR 04-5920. PubMed ID: 15592417. Show all entries for this paper.

Displaying record number 60621

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HXB2 Location	Gag(143-152) p24(11-20) DNA(1216..1245)	Gag Epitope Map
Author Location	p24(11-20)
Epitope	`VHQAISPRTL`	Epitope Alignment
Epitope Name	VL-10
Subtype	B, C
Species (MHC/HLA)	human(B15:10, C03)
Immunogen	HIV-1 infection
Experimental methods
Keywords

Notes

Subtype-specific substitutions in two HIV Gag regions (fragments F1, amino acids (aa) 5-46, and F2 aa 100-143) result in novel adaptations that affect epitope processing efficiency. Consequent low epitope abundance is associated with weak T cell response and greater immune escape and therefore with disease state. Conversely, epitopes from these regions when restricted by certain rare HLA I alleles, are associated with greater production and resulting CTL response, therefore delayed disease progression.
Most epitopes were found to be processed as precursors with N-terminal extensions beyond the optimal epitope. Only 4 epitopes in this region were associated with delayed progression - HLA-B*5701/03-restricted IW9 (ISPRTLNAW), KF11 (KAFSPEVIPMF), TW10 (TSTLQEQIGW) and HLA-B*2705-restricted KK10 (KRWIILGLNK).
Regardless of ethnic group, white or African, a significant three-way interaction was found between HLA allelic frequency, epitope yield and ethnicity for epitopes of N-terminal fragment F1 (NF1) and C-terminal as well as middle F2 (CF2 and MF2). C-terminal F1 (CF1) epitope yields however, showed a significant difference between ethnic groups. Also, for identical HLA-mediated selection pressure in both Africans and whites, HIV-C had a lower concentration than HIV-B, especially at lower HLA allelic frequencies.
Epitope VHQAISPRTL , VL-10, is from fragment F1, and restricted by HLA-B*1510 and Cw*03(Cw*03 has occasionally been reported to also bind VL10 but binding studies disagree about whether HLA-Cw*03 can bind VL10 or not. (Zappacosta et al., 1997; Zarling and Lee, 1998). 2% LQGQMVHQAISPRTL, VL-10's subtype B, natural processed epitope form, accumulated after 4 hours processing by constitutive proteasomes, and 2% with immuno proteasomal processing. For subtype C, the natural processed form was LQGQMVHQAISPRTL, 1% 4h post-constitutive- and 1% post-immuno-proteasomal processing.

References

Tenzer2014 Stefan Tenzer, Hayley Crawford, Phillip Pymm, Robert Gifford, Vattipally B. Sreenu, Mirjana Weimershaus, Tulio de Oliveira, Anne Burgevin, Jan Gerstoft, Nadja Akkad, Daniel Lunn, Lars Fugger, John Bell, Hansjörg Schild, Peter van Endert, and Astrid K. N. Iversen. HIV-1 Adaptation to Antigen Processing Results in Population-Level Immune Evasion and Affects Subtype Diversification. Cell Rep., 7(2):448-463, 24 Apr 2014. PubMed ID: 24726370. Show all entries for this paper.

Displaying record number 59425

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HXB2 Location	Gag(143-152) p24(11-20) DNA(1216..1245)	Gag Epitope Map
Author Location	Gag(143-152)
Epitope	`VHQAISPRTL`	Epitope Alignment
Epitope Name	Gag143-152
Species (MHC/HLA)	human(C*03:04)
Immunogen	in vitro stimulation or selection
Experimental methods	Degranulation, CD107a and b cell surface mobilization, Other, T2 stabilization assay
Keywords	computational epitope prediction, NK cells

Notes

HIV may evade killer T cells through the selection of variants that interfere with the interactions of inhibitory killer cell Ig-like receptors (KIRs). This study identified Cw*0304-restricted epitopes that alter KIR2DL2 binding. Seven peptides were selected that were able to bind to Cw*0304. Three of the seven were previously described.
VHQAISPRTL was identified based on its binding to Cw*0304 in vitro. A previously-identified 22-mer had included this epitope. Gag144-152 enables binding of KIR2DL2 to HLA-Cw*0304 and results in inhibition of KIR2DL2+ primary NK cells.

References

vanTeijlingen2014 Nienke H. van Teijlingen, Angelique Hölzemer, Christian Körner, Wilfredo F. García-Beltrán, Jamie L. Schafer, Lena Fadda, Todd J. Suscovich, Christian Brander, Mary Carrington, David T Evans, Debbie van Baarle, and Marcus Altfeld. Sequence Variations in HIV-1 p24 Gag-Derived Epitopes Can Alter Binding of KIR2DL2 to HLA-C*03:04 and Modulate Primary Natural Killer Cell Function. AIDS, 28(10):1399-1408, 19 Jun 2014. PubMed ID: 24785948. Show all entries for this paper.

Displaying record number 58955

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HXB2 Location	Gag(143-152) p24(11-20) DNA(1216..1245)	Gag Epitope Map
Author Location	Gag(143-152)
Epitope	`VHQAISPRTL`	Epitope Alignment
Subtype	C
Species (MHC/HLA)	human
Immunogen	HIV-1 infection
Patient MHC/HLA	CAP210: A68:02, B15:10, C*03:04
Country	South Africa
Experimental methods	CD8 T-cell Elispot - IFNγ, Other
Keywords	escape, acute/early infection

Notes

Amino acid mutations in HIV-1 subtype C were tracked within the first 6 months following transmission in 5 subjects. Early, rapid adaptations (55 mutations) to the viral genome were predominantly driven by CTL pressure (38%), but also by antibody pressure (35%) and reversions (16%). Most CTL escape occurred in Nef, followed by Pol and Env.
Epitope VHQAISPRTL underwent mutation at amino acid A, position 4. This change was first seen 12-16 weeks after transmission. The escape was tracked in subject CAP210.
Using autologous peptides, 13/21 putative epitopes were confirmed to be responsive [see Liu et al. J. Clinical Investigation. 123:380-93 (2013) for details]

References

Abrahams2013 Melissa-Rose Abrahams, Florette K. Treurnicht, Nobubelo K. Ngandu, Sarah A. Goodier, Jinny C. Marais, Helba Bredell, Ruwayhida Thebus, Debra de Assis Rosa, Koleka Mlisana, Cathal Seoighe, Salim Abdool Karim, Clive M. Gray, and Carolyn Williamson. Rapid, Complex Adaptation of Transmitted HIV-1 Full-Length Genomes in Subtype C-Infected Individuals with Differing Disease Progression. AIDS, 27(4):507-518, 20 Feb 2013. PubMed ID: 23370465. Show all entries for this paper.

Displaying record number 61043

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HXB2 Location	Gag(143-152) p24(11-20) DNA(1216..1245)	Gag Epitope Map
Author Location	p24(11-20)
Epitope	`VHQAISPRTL`	Epitope Alignment
Subtype	C
Species (MHC/HLA)	human
Immunogen	HIV-1 infection
Patient MHC/HLA	CAP210: A68:02, B15:10, C*03:04
Country	South Africa
Experimental methods	CD8 T-cell Elispot - IFNγ, Other
Keywords	computational epitope prediction, escape, acute/early infection, mutation acquisition

Notes

17 patients with acute HIV-1 infection were followed to validate that (1) relative escape rate (2) location and identity of escape mutations and (3) epistatic effects on escape, could be predicted within a host by using a simple model based on population genetics and known fitness measures of HIV polyproteins. In general escape location was found most at residues with the lowest fitness cost and escape time correlates with early detection of mutants when T cell response was first detected.
Epitope VHQAISPRTL had a fitness cost ΔE of 2.408 and T cell response was first detected to it at 22d, escape time was 144.01 days.

References

Barton2016 John P. Barton, Nilu Goonetilleke, Thomas C. Butler, Bruce D. Walker, Andrew J. McMichael, and Arup K. Chakraborty. Relative Rate and Location of Intra-Host HIV Evolution to Evade Cellular Immunity Are Predictable. Nat. Commun., 7:11660, 23 May 2016. PubMed ID: 27212475. Show all entries for this paper.