Search CTL/CD8+ T-Cell Epitope Database

Found 2 matching records:

Displaying record number 214

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HXB2 Location	Gag(215-224) p24(83-92) DNA(1432..1461)	Gag Epitope Map
Author Location	p24(215-223 IIIB)
Epitope	VHPVHAGPIA	Epitope Alignment
		Show epitope variants
Species (MHC/HLA)	human(B55)
Immunogen	HIV-1 infection
Experimental methods
Keywords

Notes

• HIV IIIB proteins were used to define the range of CTL epitopes recognized by 3 lab workers accidentally infected with HIV-1 IIIB.
• LHPVHAGPVA, a variant found in HIV-1 PH136, was also recognized.
• LHPVHAGPIA, a variant found in HIV-1 RF, was also recognized.
• LHPVHAGPIT, a variant found in HIV-1 MN, was also recognized.
• LHPAQAGPIA, a variant found in HIV-1 JH3, was recognized at high peptide concentrations.

References

Sipsas1997 N. V. Sipsas, S. A. Kalams, A. Trocha, S. He, W. A. Blattner, B. D. Walker, and R. P. Johnson. Identification of Type-Specific Cytotoxic T Lymphocyte Responses to Homologous Viral Proteins in Laboratory Workers Accidentally Infected with HIV-1. J. Clin. Invest., 99:752-762, 1997. To examine a situation where the autologous strain and the reference reagents would be the same, the CTL response of three lab workers accidentally infected with HIV IIIB was studied. Both group specific and type specific epitopes were targets for CTL clones. One subject had a broadening of CTL response over time, using a broad range of restricting HLA class I alleles. Characterization of the cytotoxic T lymphocyte (CTL) response against HIV-1 has been limited by the use of target cells expressing viral proteins from laboratory isolates of HIV-1. This approach has favored identification of group-specific CTL responses and precluded assessment of the extent of type-specific CTL responses directed against HIV-1. Using cells expressing viral proteins from the HIV-1 IIIB strain, we performed a detailed characterization of HIV-1-specific CTL response in three laboratory workers accidentally infected with HIV-1 IIIB. Eight of the epitopes identified were group specific, lying in relatively conserved regions of Gag, reverse transcriptase, and envelope. Three type-specific epitopes were identified, two of them in highly variable regions of envelope. In longitudinal studies in one subject, seven different epitopes and five different restricting HLA class I alleles were identified, with a progressive increase in the number of CTL epitopes recognized by this subject over time. Our data demonstrate that type-specific CTL responses make up a significant proportion of the host cellular immune response against HIV-1 and that a broadening of epitope specificity may occur. PubMed ID: 9045880. Show all entries for this paper.

Displaying record number 54508

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HXB2 Location	Gag(215-224) p24(83-92) DNA(1432..1461)	Gag Epitope Map
Author Location	Gag(215-224)
Epitope	VHPVHAGPIA	Epitope Alignment
Species (MHC/HLA)	human(B55)
Immunogen	HIV-1 infection
Patient MHC/HLA	A11, A24, B55, B56
Country	United Kingdom
Experimental methods	Flow cytometric T-cell cytokine assay, Other
Keywords	HAART, ART, immunodominance, TCR usage, memory cells

Notes

• The effect of HAART on the population size, phenotype and function of HIV- and HCMV-specific CTL clones was analyzed. It was determined that the clonal composition of gag and env HIV-specific CD8 T-cells did not change after HAART. Following HAART, the size of immunodominant HIV-specific CD8 T-cell clones was found to diminish even with the relative preservation of functional memory responses. Maintainance of such strong functional responses implied the preferential loss of HIV-specific cells that have reduced cloning efficiency in vitro. HCMV-specific CTL clones had different kinetics and phenotypes than HIV-specific CTL clones in the same subject.

References

Weekes2006 Michael P. Weekes, Mark R. Wills, J. G. Patrick Sissons, and Andrew J. Carmichael. Large HIV-Specific CD8 Cytotoxic T-Lymphocyte (CTL) Clones Reduce Their Overall Size But Maintain High Frequencies of Memory CTL Following Highly Active Antiretroviral Therapy. Immunology, 118(1):25-38, May 2006. PubMed ID: 16630020. Show all entries for this paper.