Search CTL/CD8+ T-Cell Epitope Database

Found 3 matching records:

Displaying record number 57086

Download this epitope record as JSON.

HXB2 Location	gp160(583-592) gp41(72-81) DNA(7971..8000)	gp160 Epitope Map
Author Location	gp41(72-81 DU422)
Epitope	VERYLKDQQL	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human(A*02:01)
Immunogen	in vitro stimulation or selection
Experimental methods	CD8 T-cell Elispot - IFNγ, Chromium-release assay, Tetramer binding
Keywords	dendritic cells

Notes

• Three types of myeloid dendritic cells - monocytic dendritic cells, Langerhans cells, and interstitial dermal-intestinal dendritic cells - were shown to be able to prime CD8+ T-cell responses to HIV-1. Primed monocytic dendritic cells from 2 uninfected adult donors had broad primary T-cell reactivity, reacting with 15mers containing 42 known epitopes in Gag, Env, and Nef.
• One of the reactive 15mers contained optimal epitope VERYLKDQQL, which was restricted by HLA A*0201.

References

Colleton2009 Bonnie A. Colleton, Xiao-Li Huang, Nada M. Melhem, Zheng Fan, Luann Borowski, Giovanna Rappocciolo, and Charles R. Rinaldo. Primary Human Immunodeficiency Virus Type 1-Specific CD8+ T-Cell Responses Induced by Myeloid Dendritic Cells. J. Virol., 83(12):6288-6299, Jun 2009. PubMed ID: 19357176. Show all entries for this paper.

Displaying record number 814

Download this epitope record as JSON.

HXB2 Location	gp160(583-592) gp41(72-81) DNA(7971..8000)	gp160 Epitope Map
Author Location	gp41(583-592 PV22)
Epitope	VERYLKDQQL	Epitope Alignment
Species (MHC/HLA)	human(B14)
Immunogen	HIV-1 infection
Experimental methods
Keywords

Notes

• HIV-1 specific CTLs release γ-IFN, and α- and β-TNF.

References

Jassoy1993 C. Jassoy, T. Harrer, T. Rosenthal, B. A. Navia, J. Worth, R. P. Johnson, and B. D. Walker. Human Immunodeficiency Virus Type 1-Specific Cytotoxic T Lymphocytes Release gamma Interferon, Tumor Necrosis Factor alpha (TNF-alpha), and TNF-beta when They Encounter Their Target Antigens. J. Virol., 67:2844-2852, 1993. In this study the ability of HIV-1-specific CTL clones derived from seropositive persons to release gamma interferon (IFN-$\gamma$), tumor necrosis factor alpha (TNF-$\alpha$), and TNF-$\beta$ upon contact with target cells presenting viral antigen was assessed. Epitopes: p17: KIRLRPGGKKKYKLKHIVWASRELE, A3; gp41: VERYLKDQQL, B14 and A28, ERYLKDQQL, B14; RT: AIFQSSMTKILEPFRKQNPDIVIYQ, A11; and Nef SQRRQDILDLWIYHTQGYFPDWQNY, B13. PubMed ID: 7682629. Show all entries for this paper.

Displaying record number 55291

Download this epitope record as JSON.

HXB2 Location	gp160(583-592) gp41(72-81) DNA(7971..8000)	gp160 Epitope Map
Author Location	Env
Epitope	VERYLKDQQL	Epitope Alignment
		Show epitope variants
Subtype	B
Species (MHC/HLA)	human(B14)
Immunogen	HIV-1 infection
Patient MHC/HLA	A1, A19, B*35:01, B44, C*16, Cw7; A*02:01, A19, B14, B44, C*16, Cw8
Country	United States
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	HAART, ART, mother-to-infant transmission, rate of progression, escape, co-receptor, mutation acquisition, HLA associated polymorphism

Notes

• HIV-1 mother-to-child transmission is studied for LTNPs by comparing entire genomes from 2 mother (M1, M2) and 2 daughter (D1, D2) RNA samples of a mother-child pair over 11 years. Genetic distance was 94% between subjects' strains. Divergence in sequences was attributed to distinct HLA selection pressures as ds/dn was larger for intra- rather than inter-person sequences. 10 new mutations in D2 were found related to unique daughter HLA alleles.
• Functional ELISpot studies using D2 and Nef peptides reveal strong associations between CTL responses and escape variants, contributing to delayed progression.
• LTNP status was not related to defective virus since all viral genes were intact and CTL response did not effectively control viral load. It is supposed that genetic HLA background and HIV-1 epitope-immune response interaction account for nonprogression of disease.
• All isolates contained R77Q in Vpr, a variation associated with reduction of cellular apoptosis.
• This HLA-B14 restricted epitope, VERYLKDQQL was mutated to VERYLrDQQL in the daughter D2 isolate.

References

Reinis2007 Milan Reinis, Barbara Weiser, Carla Kuiken, Tao Dong, Dorothy Lang, Sharon Nachman, Yonghong Zhang, Sarah Rowland-Jones, and Harold Burger. Genomic Analysis of HIV Type 1 Strains Derived from a Mother and Child Pair of Long-Term Nonprogressors. AIDS Res. Hum. Retroviruses, 23(2):309-315, Feb 2007. PubMed ID: 17331038. Show all entries for this paper.