Search CTL/CD8+ T-Cell Epitope Database

Found 3 matching records:

Displaying record number 1516

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HXB2 Location	Gag(239-247) p24(107-115) DNA(1504..1530)	Gag Epitope Map
Author Location	Gag(239-247 SF2)
Epitope	TTSTLQEQI	Epitope Alignment
Species (MHC/HLA)	mouse(H-2Kd)
Immunogen	vaccine
Experimental methods
Keywords

Vaccine Details

Notes

• BALB/c mice were immunized with recombinant Listeria monocytogenes (Lm-Gag) expressing HIV-1 HXB2 Gag.
• L. monocytogenes is a gram-positive bacteria that enters the macrophage on phagocytosis and lives in the cytoplasm -- secreted L. monocytogenes antigens are processed and presented by both class I and class II pathways.

References

Mata1998 M. Mata, P. J. Travers, Q. Liu, F. R. Frankel, and Y. Paterson. The MHC Class I-Restricted Immune Response to HIV-gag in BALB/c Mice Selects a Single Epitope That Does Not Have a Predictable MHC-Binding Motif and Binds to Kd through Interactions between a Glutamine at P3 and Pocket D. J. Immunol., 161:2985-2993, 1998. PubMed ID: 9743362. Show all entries for this paper.

Displaying record number 55329

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HXB2 Location	Gag(239-247) p24(107-115) DNA(1504..1530)	Gag Epitope Map
Author Location	Gag
Epitope	TTSTLQEQI	Epitope Alignment
Epitope Name	T
Subtype	A, B, C
Species (MHC/HLA)	mouse(H-2Kd)
Immunogen	vaccine
Experimental methods	CD8 T-cell Elispot - IFNγ, Intracellular cytokine staining, Other
Keywords	subtype comparisons, vaccine-induced immune responses, variant cross-reactivity, antagonism

Vaccine Details

Notes

• Cross-clade vaccine recognition was studied using either single-, multi or several anatomically separated single-clade vaccines. Limited cross-clade response was seen with single-clade administrations. Multi-clade vaccines gave immune interference (antagonism and original antigenic sin), reducing CTL response. Simultaneously administered but anatomically separated vaccines from clades A, B, C decreased antagonism and increased immune responses.

References

Larke2007 Natasha Larke, Eung-Jun Im, Ralf Wagner, Carolyn Williamson, Anna-Lise Williamson, Andrew J. McMichael, and Tomáš Hanke. Combined Single-Clade Candidate HIV-1 Vaccines Induce T Cell Responses Limited by Multiple Forms of In Vivo Immune Interference. Eur. J. Immunol., 37(2):566-577, Feb 2007. PubMed ID: 17230443. Show all entries for this paper.

Displaying record number 57938

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HXB2 Location	Gag(239-247) p24(107-115) DNA(1504..1530)	Gag Epitope Map
Author Location
Epitope	TTSTLQEQI	Epitope Alignment
Epitope Name	G2
Subtype	A
Species (MHC/HLA)	mouse(H-2Kd)
Immunogen	vaccine
Experimental methods	CD8 T-cell Elispot - IFNγ, Cytotoxicity assay, HLA binding, Intracellular cytokine staining
Keywords	immunodominance, vaccine antigen design, optimal epitope

Vaccine Details

Notes

• Using a candidate vaccine immunogen (HIVA), immunodominant epitopes were sequentially removed to test whether subdominant responses could be protective. Subdominant epitopes induced robust responses and became immunodominant in the absence of higher-ranked epitopes. Thus vaccines focused on less-variable regions with subdominant epitopes could be effective.
• Disruption of dominant epitopes H (RGPGRAFVTI), P (IFQSSMTKI), G1 (AMQMLKDTI), and NPP (NPPIPVGDI) caused subdominant epitope G2, TTSTLQEQI, to become dominant.

References

Im2011 Eung-Jun Im, Jessie P. Hong, Yaowaluck Roshorm, Anne Bridgeman, Sven Létourneau, Peter Liljeström, Mary Jane Potash, David J. Volsky, Andrew J. McMichael, and Tomáš Hanke. Protective Efficacy of Serially Up-Ranked Subdominant CD8+ T Cell Epitopes against Virus Challenges. PLoS Pathog., 7(5):e1002041, May 2011. PubMed ID: 21625575. Show all entries for this paper.