Search CTL/CD8+ T-Cell Epitope Database

Found 2 matching records:

Displaying record number 1830

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HXB2 Location	Nef(128-135) DNA(9178..9201)	Nef Epitope Map
Author Location	Nef(128-135 LAI)
Epitope	TPGPGVRY	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human(B*07:02)
Immunogen	in vitro stimulation or selection
Experimental methods
Keywords	epitope processing

Notes

• Five naturally processed MHC class I ligands were identified in Nef in the conserved immunogenic region Nef between 123-152.
• All five could be transported by TAP, and 4/5 had N-termini that were major cleavage points for the proteasome, only one had extended precursor fragments.
• Both TPGPGVRYPL and TPGPGVRY are naturally processed ligands that can be eluted from HLA-B7 molecules, both are recognized by the same CTL, and both peptides seem to be the direct product of a proteasomal digest.
• The peptide TPGPGVRY is present in a high copy number, TPGPGVRYPL at a more moderate level, possibly due to a major cleavage site between the Y and P.

References

LucchiariHartz2000 M. Lucchiari-Hartz, P. M. van Endert, G. Lauvau, R. Maier, A. Meyerhans, D. Mann, K. Eichmann, and G. Niedermann. Cytotoxic T lymphocyte epitopes of HIV-1 Nef: Generation of multiple definitive major histocompatibility complex class I ligands by proteasomes. J. Exp. Med., 191(2):239-52, 17 Jan 2000. URL: http://www.jem.org/cgi/content/full/191/2/239. PubMed ID: 10637269. Show all entries for this paper.

Displaying record number 52961

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HXB2 Location	Nef(128-135) DNA(9178..9201)	Nef Epitope Map
Author Location	Nef(128-135)
Epitope	TPGPGVRY	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human(B7 supertype)
Immunogen	vaccine
Experimental methods	CD8 T-cell Elispot - IFNγ, Chromium-release assay, Proliferation
Keywords	vaccine-induced immune responses, variant cross-reactivity

Vaccine Details

Notes

• After injection of an anti-HIV-1 lipopeptide vaccine and the booster injections, a response was observed in >85% of 28 volunteers, and a long-term immune response was observed in >50% of the volunteers. A positive effect of QS21 adjuvant was found for B-and CD4+T-cell responses. A higher number of CD8+ T-cell epitopes was induced after a fourth boost injection. This epitope was one of the 31 epitopes that the vaccinated volunteers responded to.

References

Gahery-Segard2003 Hanne Gahéry-Ségard, Gilles Pialoux, Suzanne Figueiredo, Céline Igéa, Mathieu Surenaud, Jessintha Gaston, Helene Gras-Masse, Jean-Paul Lévy, and Jean-Gérard Guillet. Long-Term Specific Immune Responses Induced in Humans by a Human Immunodeficiency Virus Type 1 Lipopeptide Vaccine: Characterization of CD8+-T-Cell Epitopes Recognized. J. Virol., 77(20):11220-11231, Oct 2003. PubMed ID: 14512570. Show all entries for this paper.