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Displaying record number 52311

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HXB2 Location	Nef(48-56) DNA(8938..8964)	Nef Epitope Map
Author Location	Nef(58-66 JRFL)
Epitope	TAATNADCA	Epitope Alignment
Subtype	B
Species (MHC/HLA)	mouse(H-2b)
Immunogen	vaccine
Experimental methods
Keywords

Vaccine Details

Notes

• BALB/c, C3H/HeN and C57BL/6 mice were given intramuscular immunization with Nef DNA constructs -- C57BL/6 responded to this epitope.
• The Nef mutant that lacked the myristylation site (G-->A) at position 2, and the dileucine motif (L --> A at positions 174 and 175) was impaired in terms of its ability to elicit induction of Nef-specific CD4+ and CD8+ T-cell responses. The myristylation site is critical for Nef membrane localization and function, and the di-leucine motif for the down-regulation of surface CD4 molecules, and the mutation of these regions could yield a safer vaccine.
• N-terminal addition of human tissue plasminogen activator (TPA) to Nef, enhanced CD8+ T-cell responses and could compensate for the G2A, L174A, L175A mutations -- this enhanced immunogenicity correlated with enhanced levels of protein expression in transfected cells.

References

Liang2002 Xaioping Liang, Tong-Ming Fu, Hong Xie, Emilio A. Emini, and John W. Shiver. Development of HIV-1 Nef Vaccine Components: Immunogenicity Study of Nef Mutants Lacking Myristoylation and Dileucine Motif in Mice. Vaccine, 20(27-28):3413-3421, 10 Sep 2002. PubMed ID: 12213412. Show all entries for this paper.