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Displaying record number 60632

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HXB2 Location	Gag(148-158) p24(16-26) DNA(1231..1263)	Gag Epitope Map
Author Location	p24(16-26)
Epitope	SPRTLNAWVKV	Epitope Alignment
Epitope Name	SV-11
Subtype	B, C
Species (MHC/HLA)	human(A*02)
Immunogen	HIV-1 infection
Experimental methods
Keywords

Notes

• Subtype-specific substitutions in two HIV Gag regions (fragments F1, amino acids (aa) 5-46, and F2 aa 100-143) result in novel adaptations that affect epitope processing efficiency. Consequent low epitope abundance is associated with weak T cell response and greater immune escape and therefore with disease state. Conversely, epitopes from these regions when restricted by certain rare HLA I alleles, are associated with greater production and resulting CTL response, therefore delayed disease progression.
• Most epitopes were found to be processed as precursors with N-terminal extensions beyond the optimal epitope. Only 4 epitopes in this region were associated with delayed progression - HLA-B*5701/03-restricted IW9 (ISPRTLNAW), KF11 (KAFSPEVIPMF), TW10 (TSTLQEQIGW) and HLA-B*2705-restricted KK10 (KRWIILGLNK).
• Regardless of ethnic group, white or African, a significant three-way interaction was found between HLA allelic frequency, epitope yield and ethnicity for epitopes of N-terminal fragment F1 (NF1) and C-terminal as well as middle F2 (CF2 and MF2). C-terminal F1 (CF1) epitope yields however, showed a significant difference between ethnic groups. Also, for identical HLA-mediated selection pressure in both Africans and whites, HIV-C had a lower concentration than HIV-B, especially at lower HLA allelic frequencies.
• Epitope SPRTLNAWVKV , SV-11, is from fragment F1, and restricted by HLA-A*02. 100% SPRTLNAWVKV, SV-11's subtype B, natural processed epitope form, accumulated after 4 hours processing by constitutive proteasomes, and 82% with immuno proteasomal processing. For subtype C, the natural processed form was SPRTLNAWVKV,% 4h post-constitutive- and% post-immuno-proteasomal processing.

References

Tenzer2014 Stefan Tenzer, Hayley Crawford, Phillip Pymm, Robert Gifford, Vattipally B. Sreenu, Mirjana Weimershaus, Tulio de Oliveira, Anne Burgevin, Jan Gerstoft, Nadja Akkad, Daniel Lunn, Lars Fugger, John Bell, Hansjörg Schild, Peter van Endert, and Astrid K. N. Iversen. HIV-1 Adaptation to Antigen Processing Results in Population-Level Immune Evasion and Affects Subtype Diversification. Cell Rep., 7(2):448-463, 24 Apr 2014. PubMed ID: 24726370. Show all entries for this paper.

Displaying record number 55861

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HXB2 Location	Gag(148-158) p24(16-26) DNA(1231..1263)	Gag Epitope Map
Author Location	Gag
Epitope	SPRTLNAWVKV	Epitope Alignment
Subtype	A, D
Species (MHC/HLA)	human(B7)
Immunogen	HIV-1 infection
Country	Sweden
Experimental methods	CD8 T-cell Elispot - IFNγ, Other
Keywords	subtype comparisons

Notes

• By studying Gag p24 in different subtypes of subjects, previously discovered and new epitopes were identified. New T cell epitopes, both CD8 and CD4, related to the patients' HLA are described.
• T cells cross-reacted to peptides from different patient subtypes, and this cross-reactivity was distributed along the entire p24 protein.
• HLA-B7-restricted epitope SPRTLNAWVKV is from a subtype A library, and is reactive in a subtype D-carrying subject. This epitope is part of reacting peptide VTSPRTLNAWVKVIE.

References

Gudmundsdotter2008 L. Gudmundsdotter, D. Bernasconi, B. Hejdeman, E. Sandstrom, A. Alaeus, K. Lidman, B. Ensoli, B. Wahren, and S. Buttò. Cross-Clade Immune Responses to Gag p24 in Patients Infected with Different HIV-1 Subtypes and Correlation with HLA Class I and II Alleles. Vaccine, 26(40):5182-5187, 19 Sep 2008. PubMed ID: 18479789. Show all entries for this paper.