Search CTL/CD8+ T-Cell Epitope Database

Found 8 matching records:

Displaying record number 1501

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HXB2 Location	Pol(311-320) RT(156-165) DNA(3015..3044)	Pol Epitope Map
Author Location	RT(311-319 SF2)
Epitope	`SPAIFQSSMT`	Epitope Alignment
Epitope Name	P4
Species (MHC/HLA)	human(B*07)
Immunogen	HIV-1 infection
Experimental methods
Keywords	HAART, ART

Notes

A panel of 16 epitopes covering 15 class I alleles was tested in 14 HIV+ patients from an unselected Caucasian population treated with HAART, using CD8+ cell IFNgamma production to measure responses.
In general, during the first month of treatment viral load decreased and frequencies of HIV-specific CTL tripled and broadened -- eight new HIV specificities that were not previously detectable were newly detected, as were CMV specific CD8+ PBL -- but with continued viral suppression, HIV-specific responses diminished.
Viral rebounds gave different patterns of response: increases or decreases in pre-existing response, new specificities, or no change.

References

Mollet2000 L. Mollet, T. S. Li, A. Samri, C. Tournay, R. Tubiana, V. Calvez, P. Debre, C. Katlama, and B. Autran. Dynamics of HIV-specific CD8+ T lymphocytes with changes in viral load. J. Immunol., 165(3):1692-704, 1 Aug 2000. URL: http://www.jimmunol.org/v165n3/1692/1692-abs-frame.html. PubMed ID: 10903781. Show all entries for this paper.

Displaying record number 53108

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HXB2 Location	Pol(311-320) RT(156-165) DNA(3015..3044)	Pol Epitope Map
Author Location	RT Pol(311-319)
Epitope	`SPAIFQSSMT`	Epitope Alignment
Species (MHC/HLA)	human(B*07)
Immunogen	HIV-1 infection
Country	Spain
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay, Proliferation
Keywords	HAART, ART, supervised treatment interruptions (STI)

Notes

Structured treatment interruption (STI) alone is not able to control viral replication in chronically infected patients, probably due to lack of strong, maintained T-helper cell responses. HIV-1 specific CD8+ T-cell responses were shown to increase significantly until the end of the follow up, but were not correlated with viral load.
5/7 patients recognized this epitope.

References

Plana2004 Montserrat Plana, Felipe Garcia, Annette Oxenius, Gabriel M. Ortiz, Anna Lopez, Anna Cruceta, Gabriel Mestre, Emilio Fumero, Catherine Fagard, Maria Antonia Sambeat, Ferran Segura, José M. Miró, Mireia Arnedo, L. Lopalcos, Tomas Pumarola, Bernard Hirschel, Rodney E. Phillips, Douglas F. Nixon, Teresa Gallart, and Jose M. Gatell. Relevance of HIV-1-Specific CD4+ Helper T-Cell Responses During Structured Treatment Interruptions in Patients With CD4+ T-Cell Nadir Above 400/mm3. J. Acquir. Immune Defic. Syndr., 36(3):791-799, 1 Jul 2004. PubMed ID: 15213562. Show all entries for this paper.

Displaying record number 57530

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HXB2 Location	Pol(311-320) RT(156-165) DNA(3015..3044)	Pol Epitope Map
Author Location	RT(156-165)
Epitope	`SPAIFQSSMT`	Epitope Alignment
Epitope Name	S10MT
Species (MHC/HLA)	human, transgenic mouse(B*07)
Immunogen	HIV-1 infection, vaccine
Experimental methods	CD8 T-cell Elispot - IFNγ, Chromium-release assay, HLA binding
Keywords	vaccine-induced immune responses, vaccine antigen design

Vaccine Details

Vaccine type	polyepitope
Vaccine strain	multiple epitope immunogen
Vaccine component	HIV-1

Notes

The study objective was to design a vaccine construct containing HIV-1 epitopes that would generate broad CD8+ responses. Among 14 known B7-restricted epitopes, 11 induced a response in B*07 transgenic mice. Among 11 novel candidate epitopes, all induced a response in B*07 mice, and all induced a response in at least 1 human B7+ donor. Immunological recognition of these epitopes was confirmed in HLA-B*0702 HIV-1-infected individuals.
SPAIFQSSMT is a known B07 epitope that was not recognized by B*07 transgenic mice.

References

Cardinaud2009 Sylvain Cardinaud, Romain Bouziat, Pierre-Simon Rohrlich, Sophie Tourdot, Laurence Weiss, Pierre Langlade-Demoyen, Anne Burgevin, Susana Fiorentino, Peter van Endert, and François A. Lemonnier. Design of a HIV-1-Derived HLA-B07.02-Restricted Polyepitope Construct. AIDS, 23(15):1945-1954, 24 Sep 2009. PubMed ID: 19644347. Show all entries for this paper.

Displaying record number 1470

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HXB2 Location	Pol(311-320) RT(156-165) DNA(3015..3044)	Pol Epitope Map
Author Location	RT(311-319 LAI)
Epitope	`SPAIFQSSMT`	Epitope Alignment
		Show epitope variants
Epitope Name	P4
Subtype	B
Species (MHC/HLA)	human(B35)
Immunogen	HIV-1 infection
Experimental methods
Keywords	HAART, ART, escape

Notes

This epitope contains the mutation P157S which can be induced by nucleosidee reverse transcriptase inhibitors.
It was recognized by patient 252#0 in a study of the effects of therapy escape mutations on CTL recognition.

References

Samri2000 A. Samri, G. Haas, J. Duntze, J. M. Bouley, V. Calvez, C. Katlama, and B. Autran. Immunogenicity of mutations induced by nucleoside reverse transcriptase inhibitors for human immunodeficiency virus type 1-specific cytotoxic T cells. J. Virol., 74(19):9306-12, Oct 2000. URL: http://jvi.asm.org/cgi/content/full/74/19/9306. PubMed ID: 10982378. Show all entries for this paper.

Displaying record number 52069

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HXB2 Location	Pol(311-320) RT(156-165) DNA(3015..3044)	Pol Epitope Map
Author Location	RT( IIIB)
Epitope	`SPAIFQSSMT`	Epitope Alignment
		Show epitope variants
Subtype	B
Species (MHC/HLA)	human(B7)
Immunogen	HIV-1 infection
Experimental methods
Keywords	epitope processing, escape

Notes

HIV polymorphisms in the RT protein were examined relation to HLA alleles found in a population of 473 participants in the Western Australian HIV Cohort Study. 64 significant associations between polymorphisms at particular positions and HLA alleles were detected, for HLA-B7, -B12, -B35 and -B15. Fifteen of these were in positions with known epitopes, 4 in anchor residues, 11 in other positions. Six additional polymorphic sites associated with particular HLA molecules flanked known epitopes and may relate to processing.
HLA-B7+ individuals with a S162x (18/33) substitution had higher viral loads than those that did not, suggesting escape was associated with diminished immune control of viremia.

References

Moore2002b Corey B. Moore, Mina John, Ian R. James, Frank T. Christiansen, Campbell S. Witt, and Simon A. Mallal. Evidence of HIV-1 adaptation to HLA-restricted immune responses at a population level. Science, 296(5572):1439-1443, 24 May 2002. PubMed ID: 12029127. Show all entries for this paper.

Displaying record number 1628

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HXB2 Location	Pol(311-320) RT(156-165) DNA(3015..3044)	Pol Epitope Map
Author Location	Pol
Epitope	`SPAIFQSSMT`	Epitope Alignment
Species (MHC/HLA)	human(B7)
Immunogen
Experimental methods
Keywords

Notes

The program Epimatrix was used in conjunction with the program Conservatrix to identify conserved regions of HIV that might serve as epitopes.
A subset of the potential epitopes was identified that could bind to the appropriate HLA-allele, and 15 predicted B7 superfamily (HLA B7, B8, and B58) epitopes could stimulate IFNγ production in an ELISPOT assay.
SPAIFQSSMT was confirmed as a previously identified HLA-B7 epitope in this study.

References

DeGroot2001 A. S. De Groot, A. Bosma, N. Chinai, J. Frost, B. M. Jesdale, M. A. Gonzalez, W. Martin, and C. Saint-Aubin. From genome to vaccine: in silico predictions, ex vivo verification. Vaccine, 19(31):4385-95, 14 Aug 2001. PubMed ID: 11483263. Show all entries for this paper.

Displaying record number 443

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HXB2 Location	Pol(311-320) RT(156-165) DNA(3015..3044)	Pol Epitope Map
Author Location	RT(311-319 SF2)
Epitope	`SPAIFQSSMT`	Epitope Alignment
Species (MHC/HLA)	human(B7)
Immunogen
Experimental methods
Keywords	review

Notes

Pers. comm. from C. Hey and D. Ruhl to C. Brander and B. Walker.
Menendez-Arias1998, in a review, notes that this epitope includes catalytic residues in the active site of RT.

References

Brander1997 Christian Brander and Bruce Walker. Systematic Identification of Optimal HIV-1 CTL Epitopes. In Systematic Identification of Optimal HIV-1 CTL Epitopes. pages IV--1--IV--11. Los Alamos National Laboratory, Theoretical Biology and Biophysics, Los Alamos, New Mexico, 1997. URL: http://hiv-web.lanl.gov/immunology. Show all entries for this paper.

Menendez-Arias1998 L. Menendez-Arias, A. Mas, and E. Domingo. Cytotoxic T-Lymphocyte Responses to HIV-1 Reverse Transcriptase. Viral Immunol., 11:167-181, 1998. PubMed ID: 10189185. Show all entries for this paper.

Displaying record number 52885

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HXB2 Location	Pol(311-320) RT(156-165) DNA(3015..3044)	Pol Epitope Map
Author Location	Pol
Epitope	`SPAIFQSSMT`	Epitope Alignment
Epitope Name	1306
Subtype	multiple
Species (MHC/HLA)	human(B7)
Immunogen	HIV-1 infection
Patient MHC/HLA	A24, A3, B38, B7, Cw7
Country	United States
Experimental methods	T-cell Elispot
Keywords	binding affinity, computational epitope prediction

Notes

Epitopes defined using sequence parsing and matching algorithm Conservatrix, and epitope prediction tool EpiMatrix, were shown to be conserved in a broad range of HIV-1 sequences derived from different parts of the world. 31 novel highly conserved HIV-1 epitopes were found, among which four were recognized as promiscuous epitopes and five as MHC supertypes.
Estimated binding probability for SPAIFQSSMT: 13%

References

DeGroot2003 Anne S. De Groot, Bill Jesdale, William Martin, Caitlin Saint Aubin, Hakima Sbai, Andrew Bosma, Judy Lieberman, Gail Skowron, Fadi Mansourati, and Kenneth H. Mayer. Mapping Cross-Clade HIV-1 Vaccine Epitopes Using a Bioinformatics Approach. Vaccine, 21(27-30):4486-4504, 1 Oct 2003. PubMed ID: 14505932. Show all entries for this paper.