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Displaying record number 52777

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HXB2 Location	Gag(77-85) p17(77-85) DNA(1018..1044)	Gag Epitope Map
Author Location	Gag(77-85)
Epitope	SLYNTAVTL	Epitope Alignment
Species (MHC/HLA)	human(A2)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay, Tetramer binding
Keywords	responses in children

Notes

• 65 vertically HIV-1 infected children, ages 1-16, the majority undergoing ART, were analyzed in regard to their plasma viremia and CD4+ and CD8+ T-cell counts, and CD8+ T-cell responses.
• Using vaccina expressed Gag, Pol, Env, Rev, Nef in target cells in an Elispot assay, 85% of the children recognized at least one HIV antigen. Strong CD8+ T-cell responses were directed against Pol, followed by Gag and Nef. Children younger than 4 had significantly weaker responses (7/14 had no response) than older children (only 1/32 had no response, and responses were greater in magnitude).
• SLYNTVATL and ILKEPVHGV tetramers were used to quantitate specific responses. 49 children in an expanded cohort carried HLA-A2. 1/11 children under 3 years of age had detectable CD8+ T-cell responses to SLYNTVATL, 2/11 to ILKEPVHGV. Among children over 3, 11/38 recognized SLYNTVATL and 9/38 recognized ILKEPVHGV.
• Older children that maintained a CD4 count greater than 400 cells/ul tended to have stronger CTL responses.

References

Sandberg2003 Johan K. Sandberg, Noam M. Fast, Kimberly A. Jordan, Scott N. Furlan, Jason D. Barbour, Glenn Fennelly, Joanna Dobroszycki, Hans M. L. Spiegel, Andrew Wiznia, Michael G. Rosenberg, and Douglas F. Nixon. HIV-Specific CD8+ T Cell Function in Children witH Vertically Acquired HIV-1 Infection is Critically Influenced by Age and the State of the CD4+ T Cell Compartment. J. Immunol., 170(8):4403-4410, Apr 2003. PubMed ID: 12682278. Show all entries for this paper.