Search CTL/CD8+ T-Cell Epitope Database

Found 12 matching records:

Displaying record number 1751

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HXB2 Location	gp160(813-822) gp41(302-311) DNA(8661..8690)	gp160 Epitope Map
Author Location	gp41(814-823 CM243)
Epitope	`SLLNATAIAV`	Epitope Alignment
Subtype	CRF01_AE
Species (MHC/HLA)	human(A*02)
Immunogen	HIV-1 infection
Experimental methods
Keywords	subtype comparisons

Notes

More than half of a cohort of HIV+ female sex workers (FSW) from Northern Thailand were HLA-A*11 positive, and this study concentrated on A*11 epitopes in this group, although E clade versions of previously defined B-clade A*02 and A*24 epitopes were also tested.
1/4 tested FSWs recognized the E clade version of this epitope, which differs from the previously defined B clade version by one amino acid, SLLNATDIAV.
This epitope was somewhat conserved 4/8 subtypes: CRF01 (E), B, D, and F.

References

Bond2001 K. B. Bond, B. Sriwanthana, T. W. Hodge, A. S. De Groot, T. D. Mastro, N. L. Young, N. Promadej, J. D. Altman, K. Limpakarnjanarat, and J. M. McNicholl. An HLA-directed molecular and bioinformatics approach identifies new HLA-A11 HIV-1 subtype E cytotoxic T lymphocyte epitopes in HIV-1-infected Thais. AIDS Res. Hum. Retroviruses, 17(8):703-17, 20 May 2001. PubMed ID: 11429111. Show all entries for this paper.

Displaying record number 60263

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HXB2 Location	gp160(813-822) gp41(302-311) DNA(8661..8690)	gp160 Epitope Map
Author Location	Env(813-822)
Epitope	`SLLNATAIAV`	Epitope Alignment
Epitope Name	SAV10
Species (MHC/HLA)	human(A*02)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope, chronic infection

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
SLLNATAIAV had a significantly positive association with HLA A*02; it was recognized by 23/134 A*02-positive subjects and 9/190 A*02-negative subjects.

References

Pereyra2014 Florencia Pereyra, David Heckerman, Jonathan M. Carlson, Carl Kadie, Damien Z. Soghoian, Daniel Karel, Ariel Goldenthal, Oliver B. Davis, Charles E. DeZiel, Tienho Lin, Jian Peng, Alicja Piechocka, Mary Carrington, and Bruce D. Walker. HIV Control Is Mediated in Part by CD8+ T-Cell Targeting of Specific Epitopes. J. Virol., 88(22):12937-12948, Nov 2014. PubMed ID: 25165115. Show all entries for this paper.

Displaying record number 54563

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HXB2 Location	gp160(813-822) gp41(302-311) DNA(8661..8690)	gp160 Epitope Map
Author Location	Env(813-822)
Epitope	`SLLNATAIAV`	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human(A*02)
Immunogen	HIV-1 infection
Country	United States
Experimental methods	Intracellular cytokine staining, Other
Keywords	rate of progression, escape, mutation acquisition

Notes

To correlate evolving HIV-1 populations with HLA-A2 restricted immune responses for epitopes in Gag, Pol, Env and Nef, 11 treatment-naive subjects were longitudinally studied. Results show that increased viral load is often associated with broad CTL responses early in infection that persist as an "immunological footprint". Conversely, early, restricted responses may help limit viral load. Phylogenetic and functional evidence of viral escape is seen in Gag, Nef and Pol. Gag and Nef show flanking epitope changes restricted by non-HLA-A2 alleles. As far as modes of viral adaptation, CTL responses were seen to develop both against static viral populations resulting in viral evolution to HLA-A2 as well as against existing mutants resulting in reselection of consensus B-like variants. This study reinforces that CTL immune responses detected are not necessarily beneficial to patients, but may be "footprints" from early effective responses that the virus has since escaped.
Responses to epitope SLLNATAIAV were seen in early chronic infection. This was one of the epitopes targeted by broad HLA-A2-restricted CTL responses.

References

Karlsson2007 Annika C. Karlsson, Astrid K. N. Iversen, Joan M. Chapman, Tulio de Oliviera, Gerald Spotts, Andrew J. McMichael, Miles P. Davenport, Frederick M. Hecht, and Douglas F. Nixon. Sequential Broadening of CTL Responses in Early HIV-1 Infection Is Associated with Viral Escape. PLoS ONE, 2:e225, 2007. PubMed ID: 17311088. Show all entries for this paper.

Displaying record number 54223

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HXB2 Location	gp160(813-822) gp41(302-311) DNA(8661..8690)	gp160 Epitope Map
Author Location	Env
Epitope	`SLLNATAIAV`	Epitope Alignment
Epitope Name	A2-SAV10(Env)
Species (MHC/HLA)	human(A2)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	rate of progression, immunodominance, early-expressed proteins

Notes

Immunodominant HIV epitopes in early, primary infection of CTLs were identified and the relative contributions of immune responses restricted to individual HLA class I molecules were studied.
This epitope-HLA combination was recognized. The most frequently recognized epitopes also elicited the greatest CTL response.
HIV-1 Epitope sequence variability was not correlated with immunodominant versus immune sub-dominant responses.
HLA-B*57 and HLA-B*27 restricted responses were the strongest in these early and acute infections - due to dominant responses to a single epitope in each case (TW10 for B*57 and KK10 for B*27).
In the presence of HLA-B*57 and HLA-B*27 restricted responses, the relative contributions of other restricting HLA dropped greatly.

References

Altfeld2006 Marcus Altfeld, Elizabeth T. Kalife, Ying Qi, Hendrik Streeck, Mathias Lichterfeld, Mary N. Johnston, Nicole Burgett, Martha E. Swartz, Amy Yang, Galit Alter, Xu G. Yu, Angela Meier, Juergen K. Rockstroh, Todd M. Allen, Heiko Jessen, Eric S. Rosenberg, Mary Carrington, and Bruce D. Walker. HLA Alleles Associated with Delayed Progression to AIDS Contribute Strongly to the Initial CD8+ T Cell Response against HIV-1. PLoS Med., 3(10):e403, Oct 2006. PubMed ID: 17076553. Show all entries for this paper.

Displaying record number 55493

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HXB2 Location	gp160(813-822) gp41(302-311) DNA(8661..8690)	gp160 Epitope Map
Author Location	gp41
Epitope	`SLLNATAIAV`	Epitope Alignment
Epitope Name	SAV10
Subtype	B
Species (MHC/HLA)	human(A2)
Immunogen	HIV-1 infection
Country	United States
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay, Tetramer binding
Keywords	superinfection

Notes

Loss of viral replication control by an elite controller after superinfection was found to coincide with rapid recombination events in 2, ~50 residue regions of Gag and Env that include immunodominant epitopes KK10 (KRWIILGLNK) and CL9 (CAPAGFAIL). Viral escape was seen at both epitopes and consequent CTL responses against the epitope-variants.
CTL responses to previously described HLA-A2-restricted SLLNATAIAV were seen, post-superinfection and -recombination.

References

Streeck2008a Hendrik Streeck, Bin Li, Art F. Y. Poon, Arne Schneidewind, Adrianne D. Gladden, Karen A. Power, Demetre Daskalakis, Suzane Bazner, Rosario Zuniga, Christian Brander, Eric S. Rosenberg, Simon D. W. Frost, Marcus Altfeld, and Todd M. Allen. Immune-Driven Recombination and Loss of Control after HIV Superinfection. J. Exp. Med., 205(8):1789-1796, 4 Aug 2008. PubMed ID: 18625749. Show all entries for this paper.

Displaying record number 57015

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HXB2 Location	gp160(813-822) gp41(302-311) DNA(8661..8690)	gp160 Epitope Map
Author Location	Env
Epitope	`SLLNATAIAV`	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human(A2)
Immunogen	HIV-1 infection
Country	Sweden
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	subtype comparisons, computational epitope prediction, vaccine antigen design

Notes

From HIV-1 sequences of multiple subtypes, bioinformatic prediction generated 174 CTL epitopes from Gag, Pol, Nef, Env and Tat restricted by HLA supertypes A1, A2, A3, A24, B7, B44 and B58; 10 previously-published A2- and B58-restricted epitopes were also included. Of these 184 epitopes, 113 were recognized experimentally in serum from 31 HIV+ patients.
While Gag, Pol, Env and Nef were highly immunogenic, only 38% of Tat epitopes elicited a response.
SLLNATAIAV was a known A2-restricted epitope. 2/31 patient(s) responded to it; both were known to carry an allele of A2 supertype. Responding patients were infected with HIV clade(s) B.
One of 10 epitopes included based on being known from the literature, not predicted.

References

Perez2008 Carina L. Pérez, Mette V. Larsen, Rasmus Gustafsson, Melissa M. Norström, Ann Atlas, Douglas F. Nixon, Morten Nielsen, Ole Lund, and Annika C. Karlsson. Broadly Immunogenic HLA Class I Supertype-Restricted Elite CTL Epitopes Recognized in a Diverse Population Infected with Different HIV-1 Subtypes. J. Immunol., 180(7):5092-5100, 1 Apr 2008. PubMed ID: 18354235. Show all entries for this paper.

Displaying record number 1285

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HXB2 Location	gp160(813-822) gp41(302-311) DNA(8661..8690)	gp160 Epitope Map
Author Location	gp41( SF2)
Epitope	`SLLNATAIAV`	Epitope Alignment
Epitope Name	SV10
Species (MHC/HLA)	human(A2)
Immunogen	HIV-1 infection
Experimental methods
Keywords	acute/early infection

Notes

Dominant CTL epitope in acute infection of patient AC13-- response to this epitope corresponded to reduction of initial viremia.
Several other subdominant CTL epitopes were identified in the acute phase, but a response to SL9, SLYNTVATL, was not evident until 18 months post-presentation.

References

Goulder2001 P. J. Goulder, M. M. Addo, M. A. Altfeld, E. S. Rosenberg, Y. Tang, U. Govender, N. Mngqundaniso, K. Annamalai, T. U. Vogel, M. Hammond, M. Bunce, H. M. Coovadia, and B. D. Walker. Rapid Definition of Five Novel HLA-A*3002-Restricted Human Immunodeficiency Virus-Specific Cytotoxic T-Lymphocyte Epitopes by Elispot and Intracellular Cytokine Staining Assays. J. Virol., 75(3):1339-1347, Feb 2001. URL: http://jvi.asm.org/cgi/content/full/75/3/1339. PubMed ID: 11152507. Show all entries for this paper.

Displaying record number 1729

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HXB2 Location	gp160(813-822) gp41(302-311) DNA(8661..8690)	gp160 Epitope Map
Author Location	gp41(814-823 CM243 subtype CRF01)
Epitope	`SLLNATAIAV`	Epitope Alignment
Epitope Name	E813-82
Subtype	CRF01_AE
Species (MHC/HLA)	human(A2)
Immunogen	HIV-1 infection
Experimental methods
Keywords	HIV exposed persistently seronegative (HEPS)

Notes

This was a study of HIV-1 exposed persistently seronegative (HEPS) female sex workers in Chiang Mai, northern Thailand.
HLA-A11 is very common in this population, and was enriched among the HEPS sexworkers -- weak CTL responses were detected in 4/7 HEPS women, and CTL responses were found in 8/8 HIV+ controls, and 0/9 HIV- women that were not exposed.
This epitope was reactive in HIV+ control study subjects 125 and 144 who carried HLA-A2.

References

Sriwanthana2001 B. Sriwanthana, T. Hodge, T. D. Mastro, C. S. Dezzutti, K. Bond, H. A. Stephens, L. G. Kostrikis, K. Limpakarnjanarat, N. L. Young, S. H. Qari, R. B. Lal, D. Chandanayingyong, and J. M. McNicholl. HIV-specific cytotoxic T lymphocytes, HLA-A11, and chemokine-related factors may act synergistically to determine HIV resistance in CCR5 delta32-negative female sex workers in Chiang Rai, northern Thailand. AIDS Res. Hum. Retroviruses, 17(8):719-34, 20 May 2001. PubMed ID: 11429112. Show all entries for this paper.

Displaying record number 52091

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HXB2 Location	gp160(813-822) gp41(302-311) DNA(8661..8690)	gp160 Epitope Map
Author Location	gp41(813-822 IIIB)
Epitope	`SLLNATAIAV`	Epitope Alignment
Epitope Name	D2
Subtype	B
Species (MHC/HLA)	transgenic mouse(A2)
Immunogen	vaccine
Experimental methods
Keywords	vaccine-induced immune responses

Vaccine Details

Vaccine type	DNA, DNA with protein boost
Vaccine strain	B clade IIIB
Vaccine component	gp160, gp160ΔV3
Adjuvant	IL-12

Notes

Transgenic mice expressing a HLA-A2/Kb chimeric protein were vaccinated with a full length gp160 or with gp160deltaV3, with the V3 loop deleted. Mice given gp160deltaV3 had a broader immune response than those given gp160, with increased responses to conserved HLA-A2 epitopes in the C1 region of gp120, KLTPLCVTL, and the C-term region of gp41, SLLNATAIAV.
Greater resistance was conferred by the gp160deltaV3 than the gp160 vaccine to a challenge of vaccinia expressing heterologous gp160 from primary isolates (VI-06 and 89.6), and the resistance was conferred by CD8+ T-cells.

References

Kiszka2002 Irena Kiszka, Dariusz Kmieciak, Jaroslaw Gzyl, Toshio Naito, Elizabeth Bolesta, Aleksander Sieron, Satya P. Singh, Alagarsamy Srinivasan, Giorgio Trinchieri, Yutaro Kaneko, and Danuta Kozbor. Effect of the V3 Loop Deletion of Envelope Glycoprotein on Cellular Responses and Protection against Challenge with Recombinant Vaccinia Virus Expressing gp160 of Primary Human Immunodeficiency Virus Type 1 Isolates. J. Virol., 76(9):4222-4232, May 2002. PubMed ID: 11932387. Show all entries for this paper.

Displaying record number 60264

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HXB2 Location	gp160(813-822) gp41(302-311) DNA(8661..8690)	gp160 Epitope Map
Author Location	Env(813-822)
Epitope	`SLLNATAIAV`	Epitope Alignment
Epitope Name	SAV10
Species (MHC/HLA)	human(A*30)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope, chronic infection

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
SLLNATAIAV had a significantly positive association with HLA A*30; it was recognized by 5/40 A*30-positive subjects and 4/150 A*30-negative subjects.

References

Displaying record number 60262

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HXB2 Location	gp160(813-822) gp41(302-311) DNA(8661..8690)	gp160 Epitope Map
Author Location	Env(813-822)
Epitope	`SLLNATAIAV`	Epitope Alignment
Epitope Name	SAV10
Species (MHC/HLA)	human(C*14)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope, chronic infection

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
SLLNATAIAV had a significantly positive association with HLA C*14; it was recognized by 9/17 C*14-positive subjects and 32/324 C*14-negative subjects.

References

Displaying record number 55911

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HXB2 Location	gp160(813-822) gp41(302-311) DNA(8661..8690)	gp160 Epitope Map
Author Location	gp41
Epitope	`SLLNATAIAV`	Epitope Alignment
		Show epitope variants
Epitope Name	SV10(gp41)
Subtype	B
Species (MHC/HLA)	human
Immunogen	HIV-1 infection
Country	China
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	variant cross-reactivity, chronic infection

Notes

49 ART-naive chronically HIV-1 infected Chinese subjects' CTLs were tested with 413 OLPs in IFN-γ assays, determining optimal epitopes specific for Chinese Clade B HIV-1 sequences.
An inverse correlation was found between CTL response and viral load.
Targeted peptides have lower entropy than peptides that are not CTL targeted. This correlation is independent of HLA-restriction.
Author defined epitope SLLNATAIAV elicited an immune response in Chinese HIV-1 positive subjects as part of peptide ELKNSAVSLLNATAIAVA. This epitope differs from the previously described HLA-A2-restricted epitope sequence, SLLNATDIAV, at 1 residue, SLLNATaIAV.
2 of the 55 HLA-A2 carriers responded to SLLNATAIaV-containing peptide with average magnitude of CTL response of 270 SFC/million PBMC (author communication and Fig.1).

References

Zhai2008 Song Zhai, Yan Zhuang, Yang Song, Shu Li, Dedong Huang, Wenzhen Kang, Xinhong Li, Qi Liao, Yanhou Liu, Zhongfang Zhao, Yichen Lu, and Yongtao Sun. HIV-1-Specific Cytotoxic T Lymphocyte (CTL) Responses Against Immunodominant Optimal Epitopes Slow the Progression of AIDS in China. Curr. HIV Res., 6(4):335-350, Jun 2008. PubMed ID: 18691032. Show all entries for this paper.