Search CTL/CD8+ T-Cell Epitope Database

Found 3 matching records:

Displaying record number 52667

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HXB2 Location	Vpr(41-49) DNA(5679..5705)	Vpr Epitope Map
Author Location	Vpr
Epitope	SLGQHIYET	Epitope Alignment
Epitope Name	Vpr41
Species (MHC/HLA)	human, transgenic mouse(A2)
Immunogen	HIV-1 infection, vaccine
Experimental methods	Chromium-release assay, Flow cytometric T-cell cytokine assay, T-cell Elispot
Keywords	binding affinity, subtype comparisons, computational epitope prediction

Vaccine Details

Notes

• HLA-A2-restricted HIV-1 CTL epitopes were computationally predicted. Binding affinities for HLA-A*0204, immunogenicity in HLA-A*0201 transgenic mice, and responses to the peptides in 17 HIV-1 infected patients were tested. 31 novel conserved A2 epitopes were detected. An average of 4 epitopes were recognized per patient.
• This peptide was a good A2 binder, and induced CTL and CD8+ T-cell IFN gamma responses in mice. Responses to the peptide were not detected in 17 HIV+ HLA-A2 subjects.

References

Corbet2003 Sylvie Corbet, Henrik Vedel Nielsen, Lasse Vinner, Sanne Lauemoller, Dominic Therrien, Sheila Tang, Gitte Kronborg, Lars Mathiesen, Paul Chaplin, Søren Brunak, Søren Buus, and Anders Fomsgaard. Optimization and Immune Recognition of Multiple Novel Conserved HLA-A2, Human Immunodeficiency Virus Type 1-Specific CTL Epitopes. J. Gen. Virol., 84(Pt 9):2409-2421, Sep 2003. PubMed ID: 12917462. Show all entries for this paper.

Displaying record number 55391

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HXB2 Location	Vpr(41-49) DNA(5679..5705)	Vpr Epitope Map
Author Location
Epitope	SLGQHIYET	Epitope Alignment
		Show epitope variants
Epitope Name	Vpr 41
Species (MHC/HLA)	human(A2)
Immunogen	HIV-1 infection
Country	Denmark
Experimental methods	CD8 T-cell Elispot - IFNγ, Chromium-release assay, Flow cytometric T-cell cytokine assay, Intracellular cytokine staining
Keywords	computational epitope prediction, immunodominance, escape, variant cross-reactivity

Notes

• 11 HLA-A2+ (A*0201, A*0220, A*0234 or A*0236), patients' HIV were sequenced. Of 28 epitopes studied, most were conserved and elicited a subdominant CTL response. 9 epitopes in 7 proteins showed T-cell cross reaction. These epitopes were optimized to induce more immunogenic but cross-reacting responses.
• Epitopes in Gag, Pol, Env, Nef were more conserved than epitopes in shorter proteins Vpu, Vif, Vpr and Rev. Most natural epitopes were untargeted or targeted by 1 or 2 patients.
• Previously defined Vpr 41 SLGQHIYET, a rare HLA-A2 epitope, was found in only 1 HLA-A2+ patient and no immune response was detected to it. It was, however, recognized by 1 HLA-A2- patient.

References

Thorn2007 Mette Thorn, Sheila Tang, Dominic Therrien, Henrik Kløverpris, Lasse Vinner, Gitte Kronborg, Jan Gerstoft, Sylvie Corbet, and Anders Fomsgaard. Sequence Conservation of Subdominant HLA-A2-Binding CTL Epitopes in HIV-1 Clinical Isolates and CD8+ T-Lymphocyte Cross-Recognition May Explain the Immune Reaction in Infected Individuals. APMIS, 115(6):757-768, Jun 2007. PubMed ID: 17550385. Show all entries for this paper.

Displaying record number 55801

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HXB2 Location	Vpr(41-49) DNA(5679..5705)	Vpr Epitope Map
Author Location	Vpr(41-49)
Epitope	SLGQHIYET	Epitope Alignment
Species (MHC/HLA)	human
Immunogen	HIV-1 infection
Experimental methods
Keywords	computational epitope prediction, HLA associated polymorphism

Notes

• 976 Vpr sequences were studied from global HIV-1 isolates to comprehensively analyze amino acid polymorphisms. Since several polymorphic residues were part of CTL epitopes, HIV-1 immune evasion in Vpr is noted.
• 49 possible amino acid polymorphisms were defined in this previously published CTL epitope, SLGQHIYET.
• Using half-time estimates of disassociation of the molecule containing the epitope from specific HLA Class I molecules, 12 additional CTL epitopes were predicted across Vpr (Table 17).

References

Srinivasan2008 Alagarsamy Srinivasan, Velpandi Ayyavoo, Sundarasamy Mahalingam, Aarthi Kannan, Anne Boyd, Debduti Datta, Vaniambadi S. Kalyanaraman, Anthony Cristillo, Ronald G. Collman, Nelly Morellet, Bassel E. Sawaya, and Ramachandran Murali. A Comprehensive Analysis of the Naturally Occurring Polymorphisms in HIV-1 Vpr: Potential Impact on CTL Epitopes. Virol. J., 5:99, 2008. PubMed ID: 18721481. Show all entries for this paper.