Search CTL/CD8+ T-Cell Epitope Database

Found 6 matching records:

Displaying record number 60223

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HXB2 Location	Gag(263-272) p24(131-140) DNA(1576..1605)	Gag Epitope Map
Author Location	Gag(263-272)
Epitope	`RRWIQLGLQK`	Epitope Alignment
Epitope Name	RK10
Species (MHC/HLA)	human(B*18)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope, chronic infection

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
RRWIQLGLQK had a significantly positive association with HLA B*18; it was recognized by 3/23 B*18-positive subjects and 6/318 B*18-negative subjects.

References

Pereyra2014 Florencia Pereyra, David Heckerman, Jonathan M. Carlson, Carl Kadie, Damien Z. Soghoian, Daniel Karel, Ariel Goldenthal, Oliver B. Davis, Charles E. DeZiel, Tienho Lin, Jian Peng, Alicja Piechocka, Mary Carrington, and Bruce D. Walker. HIV Control Is Mediated in Part by CD8+ T-Cell Targeting of Specific Epitopes. J. Virol., 88(22):12937-12948, Nov 2014. PubMed ID: 25165115. Show all entries for this paper.

Displaying record number 1802

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HXB2 Location	Gag(263-272) p24(131-140) DNA(1576..1605)	Gag Epitope Map
Author Location	p24(260-299)
Epitope	`RRWIQLGLQK`	Epitope Alignment
Species (MHC/HLA)	human(B*27)
Immunogen	HIV-1 infection
Experimental methods
Keywords	rate of progression, acute/early infection

Notes

The CTL response to optimally defined CTL epitopes restricted by HLA class I A and B alleles in individuals who coexpressed HLA A*02, A*03, and B*07 was studied in eight HIV-1-infected subjects, two with acute infection, five with chronic, and one long-term non-progressor (LTNP).
2 to 17 epitopes were recognized in a given individual, A*02-restricted CTL response tended to be narrow and never dominated the response. 25/27 epitopes were targeted by at least one person.
RRWIQLGLQK was one of the B*27-restricted epitopes tested for responses in this investigation.

References

Day2001 C. L. Day, A. K. Shea, M. A. Altfeld, D. P. Olson, S. P. Buchbinder, F. M. Hecht, E. S. Rosenberg, B. D. Walker, and S. A. Kalams. Relative dominance of epitope-specific cytotoxic T-lymphocyte responses in human immunodeficiency virus type 1-infected persons with shared HLA alleles. J. Virol., 75(14):6279-91, Jul 2001. URL: http://jvi.asm.org/cgi/content/full/75/14/6279. PubMed ID: 11413294. Show all entries for this paper.

Displaying record number 262

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HXB2 Location	Gag(263-272) p24(131-140) DNA(1576..1605)	Gag Epitope Map
Author Location	p24(260-269 HIV-2)
Epitope	`RRWIQLGLQK`	Epitope Alignment
Species (MHC/HLA)	human(B27)
Immunogen
Experimental methods
Keywords

Notes

HIV-2, HLA-B*2703, S. Rowland-Jones, pers. comm.

References

Brander1996 Christian Brander and Bruce Walker. The HLA-Class I Restricted CTL Response in HIV-1 Infection: Systematic Identification of Optimal Epitopes. In The HLA-Class I Restricted CTL Response in HIV-1 Infection: Systematic Identification of Optimal Epitopes. pages IV--50--IV--60. Los Alamos National Laboratory, Theoretical Biology and Biophysics, Los Alamos, New Mexico, 1996. URL: http://hiv-web.lanl.gov/immunology. Show all entries for this paper.

Displaying record number 263

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HXB2 Location	Gag(263-272) p24(131-140) DNA(1576..1605)	Gag Epitope Map
Author Location	p24(260-269 HIV-2)
Epitope	`RRWIQLGLQK`	Epitope Alignment
Species (MHC/HLA)	human(B*27:03)
Immunogen
Experimental methods
Keywords	optimal epitope, A-list

Notes

C. Brander notes this is a B*2703 epitope.

References

Llano2019 Anuska Llano, Samandhy Cedeño, Sandra Silva Arrieta, and Christian Brander. The 2019 Optimal HIV CTL Epitopes Update: Growing Diversity in Epitope Length and HLA restriction. 2019. Show all entries for this paper.

Displaying record number 56729

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HXB2 Location	Gag(263-272) p24(131-140) DNA(1576..1605)	Gag Epitope Map
Author Location	p24
Epitope	`RRWIQLGLQK`	Epitope Alignment
Species (MHC/HLA)	human, macaque(B*27:05)
Immunogen	peptide-HLA interaction
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	binding affinity

Notes

Peptide binding repertoires of macaque Mamu-B*08 and human HLA-B*2705 were examined and found to overlap. 6 novel Mamu-B*08-restricted epitopes were found. The SIV-infected Indian rhesus macaque is a relevant model to examine elite control of HIV replication.
Known HLA-B27 T cell epitope, RRWIQLGLQK, also binds macaque Mamu-B*08 and Mamu-B*03 MHC I.

References

Loffredo2009 John T. Loffredo, John Sidney, Alex T. Bean, Dominic R. Beal, Wilfried Bardet, Angela Wahl, Oriana E. Hawkins, Shari Piaskowski, Nancy A. Wilson, William H. Hildebrand, David I. Watkins, and Alessandro Sette. Two MHC Class I Molecules Associated with Elite Control of Immunodeficiency Virus Replication, Mamu-B*08 and HLA-B*2705, Bind Peptides with Sequence Similarity. J. Immunol., 182(12):7763-7775, 15 Jun 2009. PubMed ID: 19494300. Show all entries for this paper.

Displaying record number 62808

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HXB2 Location	Gag(263-272) p24(131-140) DNA(1576..1605)	Gag Epitope Map
Author Location	p24(263-272)
Epitope	`RRWIQLGLQK`	Epitope Alignment
		Show epitope variants
Subtype	B
Species (MHC/HLA)	human
Immunogen	engineered
Experimental methods	Other, Relative replication capacity assay
Keywords	assay or method development, protective allele

Notes

NL4-3 Gag polyprotein random mutagenesis, high-throughput fitness profiling and in silico prediction of MHC-peptide binding affinity revealed that mutations in CTL epitopes presented by protective MHC-I alleles, vs. non-protective alleles, showed greater loss of experimental replicative capacity and lower levels of predicted reduction in MHC-I binding affinity. Together, these two variables could predict protectiveness of MHC-I alleles in a linear regression model. Analyses of intraperson viral evolution in 4 LTNP vs. 4 paired progressor subjects further supported paper conclusions.
After mutagenesis and passaging through human T cells, 60 single nucleotide mutations were observed in epitope RRWIQLGLQK (known to bind to B2703) & variant kRWIiLGLnK (known to bind to B2705) with a log transformation of mean fitness score, relative to NL4-3, of -0.5469.

References

Du2017 Yushen Du, Tian-Hao Zhang, Lei Dai, Xiaojuan Zheng, Aleksandr M. Gorin, John Oishi, Ting-Ting Wu, Janice M. Yoshizawa, Xinmin Li, Otto O. Yang, Otoniel Martinez-Maza, Roger Detels, and Ren Sun. Effects of Mutations on Replicative Fitness and Major Histocompatibility Complex Class I Binding Affinity Are Among the Determinants Underlying Cytotoxic-T-Lymphocyte Escape of HIV-1 Gag Epitopes. mBio, 8(6), 28 Nov 2017. PubMed ID: 29184023. Show all entries for this paper.