Search CTL/CD8+ T-Cell Epitope Database

Found 15 matching records:

Displaying record number 60208

HXB2 Location	Nef(105-114) DNA(9109..9138)	Nef Epitope Map
Author Location	Nef(105-114)
Epitope	`RRQDILDLWI`	Epitope Alignment
Epitope Name	RI10
Species (MHC/HLA)	human(A*30)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
RRQDILDLWI had a significantly positive association with HLA A*30; it was recognized by 4/34 A*30-positive subjects and 2/145 A*30-negative subjects.

References

Pereyra2014 Florencia Pereyra, David Heckerman, Jonathan M. Carlson, Carl Kadie, Damien Z. Soghoian, Daniel Karel, Ariel Goldenthal, Oliver B. Davis, Charles E. DeZiel, Tienho Lin, Jian Peng, Alicja Piechocka, Mary Carrington, and Bruce D. Walker. HIV Control Is Mediated in Part by CD8+ T-Cell Targeting of Specific Epitopes. J. Virol., 88(22):12937-12948, Nov 2014. PubMed ID: 25165115. Show all entries for this paper.

Displaying record number 60204

HXB2 Location	Nef(105-114) DNA(9109..9138)	Nef Epitope Map
Author Location	Nef(105-114)
Epitope	`RRQDILDLWI`	Epitope Alignment
Epitope Name	RI10
Species (MHC/HLA)	human(B*13)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
RRQDILDLWI had a significantly positive association with HLA B*13; it was recognized by 9/15 B*13-positive subjects and 41/326 B*13-negative subjects.

References

Displaying record number 55615

HXB2 Location	Nef(105-114) DNA(9109..9138)	Nef Epitope Map
Author Location	Nef
Epitope	`RRQDILDLWI`	Epitope Alignment
Epitope Name	RI10
Subtype	B
Species (MHC/HLA)	human(B27)
Immunogen	HIV-1 infection
Country	Netherlands
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	computational epitope prediction

Notes

Peptide prediction programs based on MHC binding, proteasomal cleavage and TAP transport were applied to select novel epitopes absent from the Los Alamos HIV database. 22 novel epitopes were tested by IFN-gamma ELISpot and 19 (11 HLA-B27 restricted, 8 HLA-B57 restricted) induced strong positive responses.
Magnitude of response generated was similar to that of previously identified epitopes, but the number of individuals responding was lower. This could be explained by less stringent HLA-typing of subjects and perhaps high sequence variability within these novel epitopes.
RI10, RRQDILDLWI, is a known HLA-B27-restricted epitope used as a positive control for eliciting CTL IFN-gamma response.

References

Schellens2008 Ingrid M. M. Schellens, Can Kesmir, Frank Miedema, Debbie van Baarle, and José A. M. Borghans. An Unanticipated Lack of Consensus Cytotoxic T Lymphocyte Epitopes in HIV-1 Databases: The Contribution of Prediction Programs. AIDS, 22(1):33-37, 2 Jan 2008. PubMed ID: 18090389. Show all entries for this paper.

Displaying record number 1566

HXB2 Location	Nef(105-114) DNA(9109..9138)	Nef Epitope Map
Author Location	Nef(105-114 SF2)
Epitope	`RRQDILDLWI`	Epitope Alignment
Species (MHC/HLA)	human(B27)
Immunogen	HIV-1 infection
Experimental methods
Keywords	HAART, ART, acute/early infection

Notes

Therapy provided during acute infection resulted in a narrower CTL response, stronger T help response, and a less diverse viral population than was seen in individuals treated during chronic infection.
The breadth and specificity of the response was determined using ELISPOT by studying 19 individuals with pre-seroconversion therapy (Group 1), 11 individuals with primary infection but post-seroconversion therapy (Group 2), and 10 individuals who responded to HAART given during chronic infection (Group 3), using 259 overlapping peptides spanning p17, p24, RT, gp41, gp120 and Nef.
Previously described and newly defined optimal epitopes were tested for CTL response.
Number of HLA-B27+ individuals that had a CTL response to this epitope broken down by group: 0/0 group 1, 0/0 group 2, and 1/1 group 3.

References

Altfeld2001b M. Altfeld, E. S. Rosenberg, R. Shankarappa, J. S. Mukherjee, F. M. Hecht, R. L. Eldridge, M. M. Addo, S. H. Poon, M. N. Phillips, G. K. Robbins, P. E. Sax, S. Boswell, J. O. Kahn, C. Brander, P. J. Goulder, J. A. Levy, J. I. Mullins, and B. D. Walker. Cellular immune responses and viral diversity in individuals treated during acute and early HIV-1 infection. J. Exp. Med., 193(2):169-80, 15 Jan 2001. URL: http://www.jem.org/cgi/content/full/193/2/169. PubMed ID: 11148221. Show all entries for this paper.

Displaying record number 57824

HXB2 Location	Nef(105-114) DNA(9109..9138)	Nef Epitope Map
Author Location	Nef(105-114)
Epitope	`RRQDILDLWI`	Epitope Alignment
		Show epitope variants
Subtype	B
Species (MHC/HLA)	human(B*27)
Immunogen	HIV-1 infection
Country	Australia
Experimental methods	CD8 T-cell Elispot - IFNγ, Tetramer binding
Keywords	rate of progression, escape, TCR usage

Notes

CTL responses were examined in 19 B*27-positive long-term non-progressors. CTL escape at R264 in the immunodominant KK10 epitope was a major determinant of viral load. Escape mutants at other B*27-restricted epitopes were uncommon. Coreceptor polymorphisms and breadth of TCR repertoire responding to KK10 were not different between patients with versus without escape at KK10.
14/16 chronically infected B*27-positive patients had wild-type sequence for subdominant epitope RRQDILDLWI. 2/16 carried possible escape mutants RgQDILDLWI or ReQDILDLWI.

References

Ammaranond2011 Palanee Ammaranond, David J. van Bockel, Kathy Petoumenos, Marylin McMurchie, Robert Finlayson, Melanie G. Middleton, Miles P. Davenport, Vanessa Venturi, Kazuo Suzuki, Linda Gelgor, John M. Kaldor, David A. Cooper, and Anthony D. Kelleher. HIV Immune Escape at an Immunodominant Epitope in HLA-B*27-Positive Individuals Predicts Viral Load Outcome. J. Immunol., 186(1):479-488, 1 Jan 2011. PubMed ID: 21115730. Show all entries for this paper.

Displaying record number 60206

HXB2 Location	Nef(105-114) DNA(9109..9138)	Nef Epitope Map
Author Location	Nef(105-114)
Epitope	`RRQDILDLWI`	Epitope Alignment
Epitope Name	RI10
Species (MHC/HLA)	human(B*27)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
RRQDILDLWI had a significantly positive association with HLA B*27; it was recognized by 8/31 B*27-positive subjects and 9/190 B*27-negative subjects.

References

Displaying record number 1805

HXB2 Location	Nef(105-114) DNA(9109..9138)	Nef Epitope Map
Author Location	Nef(105-114)
Epitope	`RRQDILDLWI`	Epitope Alignment
Species (MHC/HLA)	human(B*27)
Immunogen	HIV-1 infection
Experimental methods
Keywords

Notes

B*27-restricted CTL response was strongest to this epitope in one individual.

References

Day2001 C. L. Day, A. K. Shea, M. A. Altfeld, D. P. Olson, S. P. Buchbinder, F. M. Hecht, E. S. Rosenberg, B. D. Walker, and S. A. Kalams. Relative dominance of epitope-specific cytotoxic T-lymphocyte responses in human immunodeficiency virus type 1-infected persons with shared HLA alleles. J. Virol., 75(14):6279-91, Jul 2001. URL: http://jvi.asm.org/cgi/content/full/75/14/6279. PubMed ID: 11413294. Show all entries for this paper.

Displaying record number 1994

HXB2 Location	Nef(105-114) DNA(9109..9138)	Nef Epitope Map
Author Location	Nef
Epitope	`RRQDILDLWI`	Epitope Alignment
Epitope Name	Nef-RI10
Subtype	B
Species (MHC/HLA)	human(B*27)
Immunogen	HIV-1 infection
Country	United States
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	HAART, ART

Notes

Among HIV+ individuals who carried HLA B*27, 1/2 (50%) recognized this epitope.

References

Sabbaj2003 Steffanie Sabbaj, Anju Bansal, G. Douglas Ritter, Christopher Perkins, Bradley H. Edwards, Ethan Gough, Jianming Tang, James J. Szinger, Bette Korber, Craig M. Wilson, Richard A. Kaslow, Mark J. Mulligan, and Paul A. Goepfert. Cross-Reactive CD8+ T Cell Epitopes Identified in US Adolescent Minorities. J. Acquir. Immune Defic. Syndr., 33(4):426-438, 1 Aug 2003. PubMed ID: 12869831. Show all entries for this paper.

Displaying record number 1026

HXB2 Location	Nef(105-114) DNA(9109..9138)	Nef Epitope Map
Author Location	Nef(105-114 LAI)
Epitope	`RRQDILDLWI`	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human(B*2705)
Immunogen	HIV-1 infection
Experimental methods
Keywords	rate of progression

Notes

Defined as optimal epitope from within reactive peptide HSQRRQDILDLWIYHTQGYF [Nef(102-121 LAI)]
HLA-B*2705 is associated with slow HIV disease progression.
The HLA-B*2705 binding motif includes R at position 2, and L in the C-term position.

References

Goulder1997d P. J. Goulder, A. Edwards, R. E. Phillips, and A. J. McMichael. Identification of a Novel HLA-B*2705-Restricted Cytotoxic T Lymphocyte Epitope within a Conserved Region of HIV-1 Nef. AIDS, 11:536-538, 1997. PubMed ID: 9084804. Show all entries for this paper.

Displaying record number 1144

HXB2 Location	Nef(105-114) DNA(9109..9138)	Nef Epitope Map
Author Location	Nef(105-114 LAI)
Epitope	`RRQDILDLWI`	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human(B*2705)
Immunogen	HIV-1 infection
Experimental methods
Keywords	optimal epitope

Notes

C. Brander notes this is a B*2705 epitope.

References

Llano2019 Anuska Llano, Samandhy Cedeño, Sandra Silva Arrieta, and Christian Brander. The 2019 Optimal HIV CTL Epitopes Update: Growing Diversity in Epitope Length and HLA restriction. 2019. Show all entries for this paper.

Displaying record number 56731

HXB2 Location	Nef(105-114) DNA(9109..9138)	Nef Epitope Map
Author Location	Nef
Epitope	`RRQDILDLWI`	Epitope Alignment
Species (MHC/HLA)	human, macaque(B*2705)
Immunogen	peptide-HLA interaction
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	binding affinity

Notes

Peptide binding repertoires of macaque Mamu-B*08 and human HLA-B*2705 were examined and found to overlap. 6 novel Mamu-B*08-restricted epitopes were found. The SIV-infected Indian rhesus macaque is a relevant model to examine elite control of HIV replication.
Known HLA-B27 T cell epitope, RRQDILDLWI, also binds macaque Mamu-B*08 and Mamu-B*03 MHC I.

References

Loffredo2009 John T. Loffredo, John Sidney, Alex T. Bean, Dominic R. Beal, Wilfried Bardet, Angela Wahl, Oriana E. Hawkins, Shari Piaskowski, Nancy A. Wilson, William H. Hildebrand, David I. Watkins, and Alessandro Sette. Two MHC Class I Molecules Associated with Elite Control of Immunodeficiency Virus Replication, Mamu-B*08 and HLA-B*2705, Bind Peptides with Sequence Similarity. J. Immunol., 182(12):7763-7775, 15 Jun 2009. PubMed ID: 19494300. Show all entries for this paper.

Displaying record number 60209

HXB2 Location	Nef(105-114) DNA(9109..9138)	Nef Epitope Map
Author Location	Nef(105-114)
Epitope	`RRQDILDLWI`	Epitope Alignment
Epitope Name	RI10
Species (MHC/HLA)	human(B*50)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
RRQDILDLWI had a significantly positive association with HLA B*50; it was recognized by 1/2 B*50-positive subjects and 1/143 B*50-negative subjects.

References

Displaying record number 60205

HXB2 Location	Nef(105-114) DNA(9109..9138)	Nef Epitope Map
Author Location	Nef(105-114)
Epitope	`RRQDILDLWI`	Epitope Alignment
Epitope Name	RI10
Species (MHC/HLA)	human(C*07)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
RRQDILDLWI had a significantly positive association with HLA C*07; it was recognized by 24/105 C*07-positive subjects and 17/221 C*07-negative subjects.

References

Displaying record number 60207

HXB2 Location	Nef(105-114) DNA(9109..9138)	Nef Epitope Map
Author Location	Nef(105-114)
Epitope	`RRQDILDLWI`	Epitope Alignment
Epitope Name	RI10
Species (MHC/HLA)	human(C*14)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
RRQDILDLWI had a significantly positive association with HLA C*14; it was recognized by 3/11 C*14-positive subjects and 6/179 C*14-negative subjects.

References

Displaying record number 60210

HXB2 Location	Nef(105-114) DNA(9109..9138)	Nef Epitope Map
Author Location	Nef(105-114)
Epitope	`RRQDILDLWI`	Epitope Alignment
Epitope Name	RI10
Species (MHC/HLA)	human(C*18)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
RRQDILDLWI had a significantly positive association with HLA C*18; it was recognized by 1/5 C*18-positive subjects and 0/138 C*18-negative subjects.