Search CTL/CD8+ T-Cell Epitope Database

Found 7 matching records:

Displaying record number 60199

HXB2 Location	Pol(113-122) Protease(57-66) DNA(2421..2450)	Pol Epitope Map
Author Location	Pol(113-122)
Epitope	`RQYDQILIEI`	Epitope Alignment
Epitope Name	RI10
Species (MHC/HLA)	human(A*11)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
RQYDQILIEI had a significantly positive association with HLA A*11; it was recognized by 4/29 A*11-positive subjects and 9/297 A*11-negative subjects.

References

Pereyra2014 Florencia Pereyra, David Heckerman, Jonathan M. Carlson, Carl Kadie, Damien Z. Soghoian, Daniel Karel, Ariel Goldenthal, Oliver B. Davis, Charles E. DeZiel, Tienho Lin, Jian Peng, Alicja Piechocka, Mary Carrington, and Bruce D. Walker. HIV Control Is Mediated in Part by CD8+ T-Cell Targeting of Specific Epitopes. J. Virol., 88(22):12937-12948, Nov 2014. PubMed ID: 25165115. Show all entries for this paper.

Displaying record number 60200

HXB2 Location	Pol(113-122) Protease(57-66) DNA(2421..2450)	Pol Epitope Map
Author Location	Pol(113-122)
Epitope	`RQYDQILIEI`	Epitope Alignment
Epitope Name	RI10
Species (MHC/HLA)	human(A*32)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
RQYDQILIEI had a significantly positive association with HLA A*32; it was recognized by 3/24 A*32-positive subjects and 6/273 A*32-negative subjects.

References

Displaying record number 56382

HXB2 Location	Pol(113-122) Protease(57-66) DNA(2421..2450)	Pol Epitope Map
Author Location	Protease(57-66)
Epitope	`RQYDQILIEI`	Epitope Alignment
Epitope Name	RI10
Subtype	B
Species (MHC/HLA)	human(B*13)
Immunogen	HIV-1 infection
Country	Australia, Canada, Germany, United States
Experimental methods
Keywords	escape, HLA associated polymorphism

Notes

98 newly infected patients were studied to track early host HLA and Gag/Pol/Nef epitope immune responses' association with viral evolution. Response hierarchies were the same as those for later infection, and 50% of the most rapidly substituting epitopes were restricted by protective HLA, e.g. Gag B*57-associated mutations which were the most rapidly reverting upon transmission to a non-B*57-host. While there are great differences in mutation rates and reversions across the HIV proteome, most early viral change is driven by immune pressure.
HLA-driven epitope evolution was seen in 80% of published CTL epitopes.
HLA-B*13-associated substitutions within optimally defined epitope RQYDQILIEI are at positions L7 and E9, RQYDQIlIeI. RI10 was the 5th most rapidly escaping epitope.

References

Brumme2008 Zabrina L. Brumme, Chanson J. Brumme, Jonathan Carlson, Hendrik Streeck, Mina John, Quentin Eichbaum, Brian L Block, Brett Baker, Carl Kadie, Martin Markowitz, Heiko Jessen, Anthony D. Kelleher, Eric Rosenberg, John Kaldor, Yuko Yuki, Mary Carrington, Todd M. Allen, Simon Mallal, Marcus Altfeld, David Heckerman, and Bruce D. Walker. Marked Epitope- and Allele-Specific Differences in Rates of Mutation in Human Immunodeficiency Type 1 (HIV-1) Gag, Pol, and Nef Cytotoxic T-Lymphocyte Epitopes in Acute/Early HIV-1 Infection. J. Virol., 82(18):9216-9227, Sep 2008. PubMed ID: 18614631. Show all entries for this paper.

Displaying record number 54093

HXB2 Location	Pol(113-122) Protease(57-66) DNA(2421..2450)	Pol Epitope Map
Author Location
Epitope	`RQYDQILIEI`	Epitope Alignment
Epitope Name	RI10
Species (MHC/HLA)	human(B*13)
Immunogen
Experimental methods
Keywords	optimal epitope

Notes

C. Brander notes this is a B*13 epitope. Variant RQYDQIpIEI also noted.

References

Llano2019 Anuska Llano, Samandhy Cedeño, Sandra Silva Arrieta, and Christian Brander. The 2019 Optimal HIV CTL Epitopes Update: Growing Diversity in Epitope Length and HLA restriction. 2019. Show all entries for this paper.

Displaying record number 60198

HXB2 Location	Pol(113-122) Protease(57-66) DNA(2421..2450)	Pol Epitope Map
Author Location	Pol(113-122)
Epitope	`RQYDQILIEI`	Epitope Alignment
Epitope Name	RI10
Species (MHC/HLA)	human(B*13)
Immunogen	HIV-1 infection
Experimental methods	CD8 T-cell Elispot - IFNγ, Flow cytometric T-cell cytokine assay
Keywords	rate of progression, optimal epitope

Notes

Correlation between HLA class I and spontaneous control of HIV-1 was significant for 7-8 epitopes when 341 chronic, HIV-infected subjects, divided into controllers and non-controllers, were tested for EliSpot responses to 257 optimally defined epitopes. 21 epitopes were associated with viral progression. Protective epitopes tend to cluster in regions of critical stability for the protein, therefore constraining escape.
RQYDQILIEI had a significantly positive association with HLA B*13; it was recognized by 4/15 B*13-positive subjects and 13/326 B*13-negative subjects.

References

Displaying record number 54586

HXB2 Location	Pol(113-122) Protease(57-66) DNA(2421..2450)	Pol Epitope Map
Author Location	Pol(113-122)
Epitope	`RQYDQILIEI`	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human(B*13)
Immunogen	HIV-1 infection
Patient MHC/HLA	A0301, A3001, B1301, B1402, Cw0602, Cw0802
Country	South Africa
Experimental methods	CD8 T-cell Elispot - IFNγ, Intracellular cytokine staining
Keywords	epitope processing, escape, mutation acquisition, viral fitness and reversion, HLA associated polymorphism

Notes

To determine whether HLA-B*13-restricted CTL responses could partially explain low viremic loads in patients, a cohort of chronic C-clade infected subjects was studied. 6 novel B*13-restricted CTL epitopes were defined from both C- and B-clade viruses, 3 of which were within Gag. B*13-restricted CTL responses correlated with lower viremia, with most immunity targeted against Gag epitopes (p15, p17, p24). Gag epitope escape variants may exact a high viral fitness cost, mutations being seen within and around the optimal epitope.

References

Honeyborne2007 Isobella Honeyborne, Andrew Prendergast, Florencia Pereyra, Alasdair Leslie, Hayley Crawford, Rebecca Payne, Shabashini Reddy, Karen Bishop, Eshia Moodley, Kriebashnie Nair, Mary van der Stok, Noel McCarthy, Christine M. Rousseau, Marylyn Addo, James I. Mullins, Christian Brander, Photini Kiepiela, Bruce D. Walker, and Philip J. R. Goulder. Control of Human Immunodeficiency Virus Type 1 Is Associated with HLA-B*13 and Targeting of Multiple Gag-Specific CD8+ T-Cell Epitopes. J. Virol., 81(7):3667-3672, Apr 2007. PubMed ID: 17251285. Show all entries for this paper.

Displaying record number 61226

HXB2 Location	Pol(113-122) Protease(57-66) DNA(2421..2450)	Pol Epitope Map
Author Location	Protease(57-66)
Epitope	`RQYDQILIEI`	Epitope Alignment
		Show epitope variants
Epitope Name	RI10
Subtype	B
Species (MHC/HLA)	human(B*1302)
Immunogen	HIV-1 infection
Experimental methods	Relative replication capacity assay
Keywords	class I down-regulation by Nef, escape, novel epitope

Notes

Protective HLA-B*13 was studied by following the 10 most common polymorphisms associated with it. Conserved Gag and Nef B*13-restricted epitopes were shown to have fitness costs when mutated and this makes B*13 a highly protective HLA. In addition, escape-substitutions E24Q and Q107R together, but not separately, impair the ability of Nef to downregulate the MHCI molecule. A novel B*13-restricted Gag p24 epitope GQMVHQAI was identified. Also, Gag-K436R is a compensatory mutation to escape GagI437L.
Epitope RI10, RQYDQILIEI, contains the B*13-associated polymorphism L63S.

References

Shahid2015 Aniqa Shahid, Alex Olvera, Gursev Anmole, Xiaomei T. Kuang, Laura A. Cotton, Montserrat Plana, Christian Brander, Mark A. Brockman, and Zabrina L. Brumme. Consequences of HLA-B*13-Associated Escape Mutations on HIV-1 Replication and Nef Function. J. Virol., 89(22):11557-11571, Nov 2015. PubMed ID: 26355081. Show all entries for this paper.