Search CTL/CD8+ T-Cell Epitope Database

Found 5 matching records:

Displaying record number 54584

HXB2 Location	Nef(106-114) DNA(9112..9138)	Nef Epitope Map
Author Location	Nef(106-114)
Epitope	`RQDILDLWV`	Epitope Alignment
Subtype	B, C
Species (MHC/HLA)	human(B*13)
Immunogen	HIV-1 infection
Patient MHC/HLA	A0301, A3001, B1301, B1402, Cw0602, Cw0802
Country	South Africa
Experimental methods	CD8 T-cell Elispot - IFNγ, Intracellular cytokine staining
Keywords	epitope processing, escape, mutation acquisition, viral fitness and reversion, HLA associated polymorphism

Notes

To determine whether HLA-B*13-restricted CTL responses could partially explain low viremic loads in patients, a cohort of chronic C-clade infected subjects was studied. 6 novel B*13-restricted CTL epitopes were defined from both C- and B-clade viruses, 3 of which were within Gag. B*13-restricted CTL responses correlated with lower viremia, with most immunity targeted against Gag epitopes (p15, p17, p24). Gag epitope escape variants may exact a high viral fitness cost, mutations being seen within and around the optimal epitope.

References

Honeyborne2007 Isobella Honeyborne, Andrew Prendergast, Florencia Pereyra, Alasdair Leslie, Hayley Crawford, Rebecca Payne, Shabashini Reddy, Karen Bishop, Eshia Moodley, Kriebashnie Nair, Mary van der Stok, Noel McCarthy, Christine M. Rousseau, Marylyn Addo, James I. Mullins, Christian Brander, Photini Kiepiela, Bruce D. Walker, and Philip J. R. Goulder. Control of Human Immunodeficiency Virus Type 1 Is Associated with HLA-B*13 and Targeting of Multiple Gag-Specific CD8+ T-Cell Epitopes. J. Virol., 81(7):3667-3672, Apr 2007. PubMed ID: 17251285. Show all entries for this paper.

Displaying record number 56408

HXB2 Location	Nef(106-114) DNA(9112..9138)	Nef Epitope Map
Author Location	Nef(106-114)
Epitope	`RQDILDLWV`	Epitope Alignment
Epitope Name	RV9
Subtype	B
Species (MHC/HLA)	human(B*13)
Immunogen	HIV-1 infection
Country	Australia, Canada, Germany, United States
Experimental methods
Keywords	HLA associated polymorphism

Notes

98 newly infected patients were studied to track early host HLA and Gag/Pol/Nef epitope immune responses' association with viral evolution. Response hierarchies were the same as those for later infection, and 50% of the most rapidly substituting epitopes were restricted by protective HLA, e.g. Gag B*57-associated mutations which were the most rapidly reverting upon transmission to a non-B*57-host. While there are great differences in mutation rates and reversions across the HIV proteome, most early viral change is driven by immune pressure.
HLA-driven epitope evolution was seen in 80% of published CTL epitopes.
HLA-B*13-associated substitution within optimally defined epitope RQDILDLWV is at position Q2, RqDILDLWV.

References

Brumme2008 Zabrina L. Brumme, Chanson J. Brumme, Jonathan Carlson, Hendrik Streeck, Mina John, Quentin Eichbaum, Brian L Block, Brett Baker, Carl Kadie, Martin Markowitz, Heiko Jessen, Anthony D. Kelleher, Eric Rosenberg, John Kaldor, Yuko Yuki, Mary Carrington, Todd M. Allen, Simon Mallal, Marcus Altfeld, David Heckerman, and Bruce D. Walker. Marked Epitope- and Allele-Specific Differences in Rates of Mutation in Human Immunodeficiency Type 1 (HIV-1) Gag, Pol, and Nef Cytotoxic T-Lymphocyte Epitopes in Acute/Early HIV-1 Infection. J. Virol., 82(18):9216-9227, Sep 2008. PubMed ID: 18614631. Show all entries for this paper.

Displaying record number 56217

HXB2 Location	Nef(106-114) DNA(9112..9138)	Nef Epitope Map
Author Location
Epitope	`RQDILDLWV`	Epitope Alignment
Epitope Name	RV9
Subtype	C
Species (MHC/HLA)	human(B*1302)
Immunogen	HIV-1 infection
Country	South Africa
Experimental methods	CD8 T-cell Elispot - IFNγ, Chromium-release assay
Keywords	rate of progression

Notes

47 HIV-1 subtype C infected patients were followed longitudinally to determine whether there was a relation between magnitude and breadth of CTL responses in primary, acute infection (measured by IFN-gamma ELISpot) and disease progression (measured as viral load at 12 months). CTL responses to Nef were dominant (targeting 6 epitopic regions), followed by responses to Gag, Pol, Rev, Vpr, Env, Vpu, Vif and finally Tat.
RQDILDLWV is a known HLA-B*1302-restricted epitope that is part of peptide IHSKRRQDILDLWVYYHTQG which elicited responses in 6/25 patients, and which belongs to peptide pool 3 that accounted for over 70% of Nef responses. Epitope RV9 was experimentally verified as optimal.

References

Gray2009 Clive M. Gray, Mandla Mlotshwa, Catherine Riou, Tiyani Mathebula, Debra de Assis Rosa, Tumelo Mashishi, Cathal Seoighe, Nobubelo Ngandu, Francois van Loggerenberg, Lynn Morris, Koleka Mlisana, Carolyn Williamson, Salim Abdool Karim, and CAPRISA 002 Acute Infection Study Team. Human Immunodeficiency Virus-Specific gamma Interferon Enzyme-Linked Immunospot Assay Responses Targeting Specific Regions of the Proteome during Primary Subtype C Infection Are Poor Predictors of the Course of Viremia and Set Point. J. Virol., 83(1):470-478, Jan 2009. PubMed ID: 18945774. Show all entries for this paper.

Displaying record number 56551

HXB2 Location	Nef(106-114) DNA(9112..9138)	Nef Epitope Map
Author Location
Epitope	`RQDILDLWV`	Epitope Alignment
Epitope Name	RV9
Species (MHC/HLA)	human(B*1302)
Immunogen
Experimental methods
Keywords	optimal epitope

Notes

C. Brander notes this is a B*1302 epitope.

References

Llano2019 Anuska Llano, Samandhy Cedeño, Sandra Silva Arrieta, and Christian Brander. The 2019 Optimal HIV CTL Epitopes Update: Growing Diversity in Epitope Length and HLA restriction. 2019. Show all entries for this paper.

Displaying record number 55988

HXB2 Location	Nef(106-114) DNA(9112..9138)	Nef Epitope Map
Author Location	Nef
Epitope	`RQDILDLWV`	Epitope Alignment
		Show epitope variants
Epitope Name	RV9(Nef)
Subtype	B
Species (MHC/HLA)	human
Immunogen	HIV-1 infection
Country	China
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	variant cross-reactivity

Notes

49 ART-naive chronically HIV-1 infected Chinese subjects' CTLs were tested with 413 OLPs in IFN-gamma assays, determining optimal epitopes specific for Chinese Clade B HIV-1 sequences.
An inverse correlation was found between CTL response and viral load.
Targeted peptides have lower entropy than peptides that are not CTL targeted. This correlation is independent of HLA-restriction.
Author defined epitope RQDILDLWV elicited an immune response in Chinese HIV-1 positive subjects as part of peptide QKRQDILDLWVYHTQGYF. This epitope differs from the previously described HLA-B13-restricted epitope RQDILDLWi at 1 residue, RQDILDLWv.
3 of the 29 HLA-B13 carriers responded to RQDILDLWv-containing peptide with average magnitude of CTL response of 50 SFC/million PBMC (author communication and Fig.1).

References

Zhai2008 Song Zhai, Yan Zhuang, Yang Song, Shu Li, Dedong Huang, Wenzhen Kang, Xinhong Li, Qi Liao, Yanhou Liu, Zhongfang Zhao, Yichen Lu, and Yongtao Sun. HIV-1-Specific Cytotoxic T Lymphocyte (CTL) Responses Against Immunodominant Optimal Epitopes Slow the Progression of AIDS in China. Curr. HIV Res., 6(4):335-350, Jun 2008. PubMed ID: 18691032. Show all entries for this paper.