Search CTL/CD8+ T-Cell Epitope Database

Found 4 matching records:

Displaying record number 57633

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HXB2 Location	Gag(22-31) p17(22-31) DNA(853..882)	Gag Epitope Map
Author Location	p17(22-31)
Epitope	`RPGGKKKYKL`	Epitope Alignment
Epitope Name	p17 22-31
Subtype	B
Species (MHC/HLA)	human(B*51:01)
Immunogen	HIV-1 infection
Country	United States
Experimental methods	CD8 T-cell Elispot - IFNγ, Intracellular cytokine staining
Keywords	dendritic cells, optimal epitope

Notes

The study showed for the first time that peptide-loaded, CD40L-matured dendritic cells from HI-1-infected persons on ART produced CD8 T cells for a much broader range and magnitude of Gag and Nef epitopes than did peptides without dendritic cells. Dendritic cells were used to reveal novel Gag and Nef epitopes.
Novel optimal B*5101-restricted epitope RPGGKKKYKL was mapped from a reactive 15mer.

References

Huang2010a Xiao-Li Huang, Zheng Fan, LuAnn Borowski, Robbie B. Mailliard, Morgane Rolland, James I. Mullins, Richard D. Day, and Charles R. Rinaldo. Dendritic Cells Reveal a Broad Range of MHC Class I Epitopes for HIV-1 in Persons with Suppressed Viral Load on Antiretroviral Therapy. PLoS One, 5(9):e12936, 2010. PubMed ID: 20886040. Show all entries for this paper.

Displaying record number 58609

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HXB2 Location	Gag(22-31) p17(22-31) DNA(853..882)	Gag Epitope Map
Author Location	p17(22-31)
Epitope	`RPGGKKKYKL`	Epitope Alignment
Epitope Name	p17 22-31
Subtype	B
Species (MHC/HLA)	human(B*51:01)
Immunogen	HIV-1 infection
Country	United States
Experimental methods
Keywords	optimal epitope, A-list

Notes

C. Brander noted this as an optimal B*5101 epitope based on data from Huang2010a.

References

Llano2019 Anuska Llano, Samandhy Cedeño, Sandra Silva Arrieta, and Christian Brander. The 2019 Optimal HIV CTL Epitopes Update: Growing Diversity in Epitope Length and HLA restriction. 2019. Show all entries for this paper.

Displaying record number 53687

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HXB2 Location	Gag(22-31) p17(22-31) DNA(853..882)	Gag Epitope Map
Author Location	p17
Epitope	`RPGGKKKYKL`	Epitope Alignment
		Show epitope variants
Subtype	B, D
Species (MHC/HLA)	human(Cw4)
Immunogen	HIV-1 infection
Patient MHC/HLA	A23, A34, B44, B53, Cw4, Cw6; M12020: A23, A34, B44, B53, C04, C06
Country	Democratic Republic of the Congo
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	subtype comparisons, variant cross-reactivity

Notes

Cross-clade CTL responses in 10 non-B HIV-1 infected individuals were evaluated. T-cell reactivity was tested against peptide pools of clade B Gag, Pol, Nef, Rev and Tat. Nine individuals demonstrated cross-reactive T-cell responses to clade A, B, and C Gag pools; 6/7 patients that responded to Nef-B also reacted to clade A and C Nef pools. An inverse correlation was observed between the sequence divergence of the HLA class I restricted epitopes and the height of the T-cell responses, which in Gag could be explained through variations in the HLA-anchor residues. 42% of the responses were directed to regions containing new epitopes.
This previously described epitope was embedded in a B clade peptide that was recognized by T-cells from an infected person carrying subtype D Gag. The autologous epitope sequence had an K5N change, RPGGnKKYKL.

References

Geels2005 Mark J. Geels, Sheri A. Dubey, Kiersten Anderson, Elly Baan, Margreet Bakker, Georgios Pollakis, William A. Paxton, John W. Shiver, and Jaap Goudsmit. Broad Cross-Clade T-Cell Responses to Gag in Individuals Infected with Human Immunodeficiency Virus Type 1 Non-B Clades (A to G): Importance of HLA Anchor Residue Conservation. J. Virol., 79(17):11247-11258, Sep 2005. PubMed ID: 16103177. Show all entries for this paper.

Displaying record number 62770

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HXB2 Location	Gag(22-31) p17(22-31) DNA(853..882)	Gag Epitope Map
Author Location	p17(22-31)
Epitope	`RPGGKKKYKL`	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human
Immunogen	engineered
Experimental methods	Other, Relative replication capacity assay
Keywords	assay or method development, protective allele

Notes

NL4-3 Gag polyprotein random mutagenesis, high-throughput fitness profiling and in silico prediction of MHC-peptide binding affinity revealed that mutations in CTL epitopes presented by protective MHC-I alleles, vs. non-protective alleles, showed greater loss of experimental replicative capacity and lower levels of predicted reduction in MHC-I binding affinity. Together, these two variables could predict protectiveness of MHC-I alleles in a linear regression model. Analyses of intraperson viral evolution in 4 LTNP vs. 4 paired progressor subjects further supported paper conclusions.
After mutagenesis and passaging through human T cells, 60 single nucleotide mutations were observed in epitope RPGGKKKYKL (known to bind to B5101) with a log transformation of mean fitness score, relative to NL4-3, of -0.3789.

References

Du2017 Yushen Du, Tian-Hao Zhang, Lei Dai, Xiaojuan Zheng, Aleksandr M. Gorin, John Oishi, Ting-Ting Wu, Janice M. Yoshizawa, Xinmin Li, Otto O. Yang, Otoniel Martinez-Maza, Roger Detels, and Ren Sun. Effects of Mutations on Replicative Fitness and Major Histocompatibility Complex Class I Binding Affinity Are Among the Determinants Underlying Cytotoxic-T-Lymphocyte Escape of HIV-1 Gag Epitopes. mBio, 8(6), 28 Nov 2017. PubMed ID: 29184023. Show all entries for this paper.