Search CTL/CD8+ T-Cell Epitope Database

Found 3 matching records:

Displaying record number 57633

HXB2 Location	Gag(22-31) p17(22-31) DNA(853..882)	Gag Epitope Map
Author Location	p17(22-31)
Epitope	RPGGKKKYKL	Epitope Alignment
Epitope Name	p17 22-31
Subtype	B
Species (MHC/HLA)	human(B*5101)
Immunogen	HIV-1 infection
Country	United States
Experimental methods	CD8 T-cell Elispot - IFNγ, Intracellular cytokine staining
Keywords	dendritic cells, optimal epitope

Notes

• The study showed for the first time that peptide-loaded, CD40L-matured dendritic cells from HI-1-infected persons on ART produced CD8 T cells for a much broader range and magnitude of Gag and Nef epitopes than did peptides without dendritic cells. Dendritic cells were used to reveal novel Gag and Nef epitopes.
• Novel optimal B*5101-restricted epitope RPGGKKKYKL was mapped from a reactive 15mer.

References

Huang2010a Xiao-Li Huang, Zheng Fan, LuAnn Borowski, Robbie B. Mailliard, Morgane Rolland, James I. Mullins, Richard D. Day, and Charles R. Rinaldo. Dendritic Cells Reveal a Broad Range of MHC Class I Epitopes for HIV-1 in Persons with Suppressed Viral Load on Antiretroviral Therapy. PLoS One, 5(9):e12936, 2010. PubMed ID: 20886040. Show all entries for this paper.

Displaying record number 58609

HXB2 Location	Gag(22-31) p17(22-31) DNA(853..882)	Gag Epitope Map
Author Location	p17(22-31)
Epitope	RPGGKKKYKL	Epitope Alignment
Epitope Name	p17 22-31
Subtype	B
Species (MHC/HLA)	human(B*5101)
Immunogen	HIV-1 infection
Country	United States
Experimental methods
Keywords	optimal epitope

Notes

• C. Brander noted this as an optimal B*5101 epitope based on data from <cite>Huang 2010a</cite>.

References

Llano2019 Anuska Llano, Samandhy Cedeño, Sandra Silva Arrieta, and Christian Brander. The 2019 Optimal HIV CTL Epitopes Update: Growing Diversity in Epitope Length and HLA restriction. 2019. Show all entries for this paper.

Displaying record number 53687

HXB2 Location	Gag(22-31) p17(22-31) DNA(853..882)	Gag Epitope Map
Author Location	p17
Epitope	RPGGKKKYKL	Epitope Alignment
		Show epitope variants
Subtype	B, D
Species (MHC/HLA)	human(Cw4)
Immunogen	HIV-1 infection
Patient MHC/HLA	A23, A34, B44, B53, Cw4, Cw6
Country	Democratic Republic of the Congo
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	subtype comparisons, variant cross-reactivity

Notes

• Cross-clade CTL responses in 10 non-B HIV-1 infected individuals were evaluated. T-cell reactivity was tested against peptide pools of clade B Gag, Pol, Nef, Rev and Tat. Nine individuals demonstrated cross-reactive T-cell responses to clade A, B, and C Gag pools; 6/7 patients that responded to Nef-B also reacted to clade A and C Nef pools. An inverse correlation was observed between the sequence divergence of the HLA class I restricted epitopes and the height of the T-cell responses, which in Gag could be explained through variations in the HLA-anchor residues. 42% of the responses were directed to regions containing new epitopes.
• This previously described epitope was embedded in a B clade peptide that was recognized by T-cells from an infected person carrying subtype D Gag. The autologous epitope sequence had an K5N change, RPGGnKKYKL.

References

Geels2005 Mark J. Geels, Sheri A. Dubey, Kiersten Anderson, Elly Baan, Margreet Bakker, Georgios Pollakis, William A. Paxton, John W. Shiver, and Jaap Goudsmit. Broad Cross-Clade T-Cell Responses to Gag in Individuals Infected with Human Immunodeficiency Virus Type 1 Non-B Clades (A to G): Importance of HLA Anchor Residue Conservation. J. Virol., 79(17):11247-11258, Sep 2005. PubMed ID: 16103177. Show all entries for this paper.