Search CTL/CD8+ T-Cell Epitope Database

Found 3 matching records:

Displaying record number 61905

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HXB2 Location	Gag(275-283) p24(143-151) DNA(1612..1638)	Gag Epitope Map
Author Location
Epitope	RMYSPVSIL	Epitope Alignment
Epitope Name	RL9
Species (MHC/HLA)	human(E*01:03)
Immunogen	HIV-1 infection
Country	Japan
Experimental methods	HLA binding, Intracellular cytokine staining
Keywords	novel epitope

Notes

• Novel HLA-E*0101 and -E*0103-restricted 9-mer peptides were identified by quantifying the cell-surface stabilization of HLA molecules upon binding as detected by anti-HLA-E-monoclonal antibodies followed by flow cytometry. Screening of >400 HIV-1 15-mer binders resulted in the determination of 4 novel peptides that give a binding signal in two assays and 5 in only one assay.
• Novel Gag p24 peptide epitope RL9, RMYSPVSIL, is presented by HLA-E*0103. Binding to HLA-E*0103 was confirmed by two assays. It was a part of 15-mer peptide C070C.
• 5 other candidate HLA-E-binding peptides NEEAAEWDR (NR9), TALSEGATP (TP9), RIRTWKSLV (RV9), YFSVPLDEG (YG9) and MYSPVSILD (MD9) positive on only one of the two assays used were also identified.

References

Hannoun2018 Zara Hannoun, Zhansong Lin, Simon Brackenridge, Nozomi Kuse, Tomohiro Akahoshi, Nicola Borthwick, Andrew McMichael, Hayato Murakoshi, Masafumi Takiguchi, and Tomáš Hanke. Identification of Novel HIV-1-Derived HLA-E-Binding Peptides. Immunol. Lett., 202:65-72, Oct 2018. PubMed ID: 30172717. Show all entries for this paper.

Displaying record number 53898

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HXB2 Location	Gag(275-283) p24(143-151) DNA(1612..1638)	Gag Epitope Map
Author Location	p24(143-151)
Epitope	RMYSPVSIL	Epitope Alignment
Subtype	B
Species (MHC/HLA)	human
Immunogen	HIV-1 infection
Country	India
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	subtype comparisons, computational epitope prediction, immunodominance

Notes

• PBMCs from HIV-1 infected Indian subjects were tested for HIV-1 Gag (with C clade peptides), Nef (with B clade peptides), and Env (clade B peptides) specific T-cell responses. 5 immunodominant regions conserved across clades were identified in Gag and Nef. 3 antigenic regions were found to be recognized by CTLs in the Indian subjects; these regions were not identified as immunodominant in studies from Africa.
• 26 epitopes were predicted within reactive peptides, of which 90% were clustered in the conserved immunodominant regions of Gag and Nef.
• 3 of the epitopes were highly conserved across clades, and 7 novel epitopes were found.
• Epitope RMYSPVSIL is highly conserved across clades with >80% conservation to subtypes A, C and D. It is predicted to be restricted by HLA-A*0201.

References

Thakar2005 Madhuri R. Thakar, Leena S. Bhonge, Samir K. Lakhashe, U. Shankarkumar, Suvarna S. Sane, Smita S. Kulkarni, Bharati A. Mahajan, and Ramesh S. Paranjape. Cytolytic T Lymphocytes (CTLs) from HIV-1 Subtype C-Infected Indian Patients Recognize CTL Epitopes From a Conserved Immunodominant Region of HIV-1 Gag and Nef. J. Infect. Dis., 192(5):749-759, 1 Sep 2005. PubMed ID: 16088824. Show all entries for this paper.

Displaying record number 58945

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HXB2 Location	Gag(275-283) p24(143-151) DNA(1612..1638)	Gag Epitope Map
Author Location	Gag
Epitope	RMYSPVSIL	Epitope Alignment
Subtype	A
Species (MHC/HLA)	human
Immunogen	HIV-1 infection
Country	Kenya, Pakistan
Experimental methods	Other
Keywords	epitope processing

Notes

• To compare epitopes generated in diverse genetic populations due to the mutations that affect respective antigen processing, 29 Kenyan and 56 Pakistani subjects infected with HIV-1 subtype A were studied. HIV-1 Gag was sequenced for mutations affecting proteasomal degradation, TAP or HLA binding and CTL epitope generation. Epitope patterns were found to be altered due to population-specific mutations in epitope-generating pathways.
• Several predicted epitopes of differential frequency were shown in this study (see Table 1). Only epitopes immunologically verified previously are annotated here.
• Epitope RMYSPVSIL was generated from HIV-1 subtype A for HLA-presentation in both Kenyan and Pakistani populations.

References

Abidi2013 Syed H. Abidi, Aniqa Shahid, Laila S. Lakhani, Muhammad R. Khanani, Peter Ojwang, Nancy Okinda, Reena Shah, Farhat Abbas, Sarah Rowland-Jones, and Syed Ali. Population-Specific Evolution of HIV Gag Epitopes in Genetically Diverged Patients. Infect. Genet. Evol., 16:78-86, Jun 2013. PubMed ID: 23403357. Show all entries for this paper.