Search CTL/CD8+ T-Cell Epitope Database

Found 5 matching records:

Displaying record number 58995

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HXB2 Location	Gag(150-158) p24(18-26) DNA(1237..1263)	Gag Epitope Map
Author Location	Gag(150-)
Epitope	RLLNAWVKV	Epitope Alignment
Epitope Name	Gag150
Subtype	A
Species (MHC/HLA)	human(A*02)
Immunogen	HIV-1 infection, vaccine
Country	Guinea-Bissau
Experimental methods	CD8 T-cell Elispot - IFNγ
Keywords	vaccine-induced immune responses, vaccine antigen design, therapeutic vaccine, chronic infection

Vaccine Details

Notes

• Therapeutic vaccination of chronically infected HIV-1 subjects was evaluated as a means of halting disease progression. A subdominant peptide vaccine containing 15 CTL epitopes and 3 helper T-cell peptides along with an adjuvant was delivered to 18 infected subjects, and this Guinea-Bissau study compared to a similar Danish one. Using IFN-γ EliSpot, 9 new T-cell responses were detected in 6/14 subjects whose viral loads (VL) remained stable. Other pre-existing responses, including CTL responses, however, were not significantly detected.
• Gag150, RLLNAWVKV, is a CTL epitope sequence used as a vaccine component.

References

Roman2013 Victor Raúl Gómez Román, Kristoffer Jarlov Jensen, Sanne Skov Jensen, Christian Leo-Hansen, Sanne Jespersen, David da Silva Té, Candida Medina Rodrigues, Christoph Mikkel Janitzek, Lasse Vinner, Terese Lea Katzenstein, Peter Andersen, Ingrid Kromann, Lars Vibe Andreasen, Ingrid Karlsson, and Anders Fomsgaard. Therapeutic Vaccination Using Cationic Liposome-Adjuvanted HIV Type 1 Peptides Representing HLA-Supertype-Restricted Subdominant T Cell Epitopes: Safety, Immunogenicity, and Feasibility in Guinea-Bissau. AIDS Res Hum Retroviruses, 29(11):1504-1512, Nov 2013. PubMed ID: 23634822. Show all entries for this paper.

Displaying record number 57135

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HXB2 Location	Gag(150-158) p24(18-26) DNA(1237..1263)	Gag Epitope Map
Author Location	Gag
Epitope	RLLNAWVKV	Epitope Alignment
Epitope Name	Gag150 T2L
Species (MHC/HLA)	human(A*02:01)
Immunogen	HIV-1 infection, vaccine
Country	Denmark
Experimental methods	Intracellular cytokine staining, Pentamer labeling
Keywords	vaccine-induced immune responses, therapeutic vaccine

Vaccine Details

Notes

• Several subdominant but conserved CD8+ and CD4+ T-cell epitopes were anchor-optimized delivered to 12 treatment-naive A*0201-positive HIV-1 patients. Previously-undetected responses specific for one or more epitopes were detected in all patients.
• RLLNAWVKV is an anchor optimized variant of RtLNAWVKV epitope. 3/12 patients showed a novel induced response to this epitope after vaccination with RLLNAWVKV. Autilogous sequence in all 12 patients was RtLNAWVKV

References

Kloverpris2009a Henrik Kloverpris, Ingrid Karlsson, Jesper Bonde, Mette Thorn, Lasse Vinner, Anders E. Pedersen, Julie L. Hentze, Betina S. Andresen, Inge M. Svane, Jan Gerstoft, Gitte Kronborg, and Anders Fomsgaard. Induction of Novel CD8+ T-Cell Responses during Chronic Untreated HIV-1 Infection by Immunization with Subdominant Cytotoxic T-Lymphocyte Epitopes. AIDS, 23(11):1329-1340, 17 Jul 2009. PubMed ID: 19528789. Show all entries for this paper.

Displaying record number 57142

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HXB2 Location	Gag(150-158) p24(18-26) DNA(1237..1263)	Gag Epitope Map
Author Location	Gag
Epitope	RLLNAWVKV	Epitope Alignment
Epitope Name	Gag150mod
Species (MHC/HLA)	transgenic mouse(A*02:01)
Immunogen	vaccine
Experimental methods	CD8 T-cell Elispot - IFNγ, Chromium-release assay, Flow cytometric T-cell cytokine assay
Keywords	immunodominance, dendritic cells

Vaccine Details

Notes

• Transgenic A*0201 mice were immunized with matured dendritic cells pulsed with 7 CTL peptides, singly or in combination. The magnitude of individual T-cell responses correlated with their individual immunogenicity.
• Of the 7 epitopes tested, RLLNAWVKV was ranked with the highest immunogenicity based on several assays.

References

Kloverpris2009 Henrik N. Kloverpris, Ingrid Karlsson, Mette Thorn, Søren Buus, and Anders Fomsgaard. Immune Hierarchy among HIV-1 CD8+ T Cell Epitopes Delivered by Dendritic Cells Depends on MHC-I Binding Irrespective of Mode of Loading and Immunization in HLA-A*0201 Mice. APMIS, 117(11):849-585, Nov 2009. PubMed ID: 19845536. Show all entries for this paper.

Displaying record number 57506

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HXB2 Location	Gag(150-158) p24(18-26) DNA(1237..1263)	Gag Epitope Map
Author Location
Epitope	RLLNAWVKV	Epitope Alignment
Epitope Name	Gag150
Species (MHC/HLA)	transgenic mouse(A2)
Immunogen	vaccine
Experimental methods	T-cell Elispot
Keywords	vaccine-induced immune responses, adjuvant comparison, vaccine antigen design

Vaccine Details

Notes

• 15 conserved CD8 epitopes from multiple proteins and 2 helper epitopes were included to the vaccine candidate AFO18 with a novel cationic CAF01 adjuvant. AFO18- CAF01 vaccine candidate was tested in HLA-A2 transgenic mice.
• T-cell responses to the included peptides were tested in IFN-γ ELISPOT, and responses were found to both CD4 and CD8 peptides.
• This CD8 peptide was included in the vaccine and induced positive responses in 8/9 transgenic HLA-A2 mice.

References

Fomsgaard2011 Anders Fomsgaard, Ingrid Karlsson, Gregers Gram, Christian Schou, Sheila Tang, Peter Bang, Ingrid Kromann, Peter Andersen, and Lars Vibe Andreasen. Development and Preclinical Safety Evaluation of a New Therapeutic HIV-1 Vaccine Based on 18 T-Cell Minimal Epitope Peptides Applying a Novel Cationic Adjuvant CAF01. Vaccine, 29(40):7067-7074, Sep 16 2011. PubMed ID: 21767590. Show all entries for this paper.

Displaying record number 58407

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HXB2 Location	Gag(150-158) p24(18-26) DNA(1237..1263)	Gag Epitope Map
Author Location	Gag
Epitope	RLLNAWVKV	Epitope Alignment
Epitope Name	Gag150
Subtype	B, CRF02_AG
Species (MHC/HLA)	human(A2)
Immunogen	HIV-1 infection
Country	Cote D'Ivoire, Denmark, Ghana, Guinea-Bissau, Mali, Senegal
Experimental methods	Intracellular cytokine staining, Pentamer labeling
Keywords	vaccine antigen design

Notes

• 15 subdominant CTL epitopes were used to study 2 new Guinea-Bissau HIV-1 isolates, a newly completed Danish subtype B genome sequence and other previously published West African sequences. Subtype analysis showed the 2 new Guinea-Bissau sequences were CRF02_AG inter-subtype recombinant forms with typical cross-over points. The 15 epitopes were restricted by common HLA alleles (A1, A2, A3, B7, B44) and were present and conserved in all sequences studied. Mean conservation of the epitopes in local strains was 76% and 70% for Denmark and West Africa respectively. All epitopes had been previously confirmed as antigenic [Corbet2003, Thorn2007, Kloverpris2009].
• Gag150 epitope, RLLNAWVKV, showed a conservation of amino acid identity of 100% for the Danish sequence and 88% for the West African sequences.

References

Corbet2003 Sylvie Corbet, Henrik Vedel Nielsen, Lasse Vinner, Sanne Lauemoller, Dominic Therrien, Sheila Tang, Gitte Kronborg, Lars Mathiesen, Paul Chaplin, Søren Brunak, Søren Buus, and Anders Fomsgaard. Optimization and Immune Recognition of Multiple Novel Conserved HLA-A2, Human Immunodeficiency Virus Type 1-Specific CTL Epitopes. J. Gen. Virol., 84(Pt 9):2409-2421, Sep 2003. PubMed ID: 12917462. Show all entries for this paper.

Kloverpris2009 Henrik N. Kloverpris, Ingrid Karlsson, Mette Thorn, Søren Buus, and Anders Fomsgaard. Immune Hierarchy among HIV-1 CD8+ T Cell Epitopes Delivered by Dendritic Cells Depends on MHC-I Binding Irrespective of Mode of Loading and Immunization in HLA-A*0201 Mice. APMIS, 117(11):849-585, Nov 2009. PubMed ID: 19845536. Show all entries for this paper.

Thorn2007 Mette Thorn, Sheila Tang, Dominic Therrien, Henrik Kløverpris, Lasse Vinner, Gitte Kronborg, Jan Gerstoft, Sylvie Corbet, and Anders Fomsgaard. Sequence Conservation of Subdominant HLA-A2-Binding CTL Epitopes in HIV-1 Clinical Isolates and CD8+ T-Lymphocyte Cross-Recognition May Explain the Immune Reaction in Infected Individuals. APMIS, 115(6):757-768, Jun 2007. PubMed ID: 17550385. Show all entries for this paper.

Vinner2011 Lasse Vinner, Birgitta Holmgren, Kristoffer J. Jensen, Joakim Esbjornsson, Marie Borggren, Julie L. Hentze, Ingrid Karlsson, Betina S. Andresen, Gregers J. Gram, Henrik Kloverpris, Peter Aaby, Zacarias José Da Silva, Eva-Maria Fenyö, and Anders Fomsgaard. Sequence Analysis of HIV-1 Isolates from Guinea-Bissau: Selection of Vaccine Epitopes Relevant in Both West African and European Countries. APMIS, 119(8):487-497, Aug 2011. PubMed ID: 21749448. Show all entries for this paper.