Search CTL/CD8+ T-Cell Epitope Database

Found 3 matching records:

Displaying record number 1583

Download this epitope record as JSON.

HXB2 Location	Tat(49-57) DNA(5975..6001)	Tat Epitope Map
Author Location	Tat(49-57)
Epitope	RKKRRQRRR	Epitope Alignment
Species (MHC/HLA)	mouse(H-2d)
Immunogen	vaccine
Experimental methods
Keywords	Th1

Vaccine Details

Notes

• DNA vaccinated BALB/c mice primed and boosted with the multiepitopic vaccine with IL18 showed lymphoproliferative responses 7 weeks post immunization.
• Strong but non-lasting HIV-specific CTL responses were detected by a Cr-release assay and DNA prime/DNA boost was more effective than DNA prime protein boost.
• Immunization with either the multiepitopic DNA or with the mixed DNA vaccine induced HIV-1 specific Th1 cytokines (IL-2 and IFN-gamma)
• Co-administration of IL18 increased T-cell responses but decreased anti-HIV antibody levels.

References

BillautMulot2001 O. Billaut-Mulot, T. Idziorek, M. Loyens, A. Capron, and G. M. Bahr. Modulation of cellular and humoral immune responses to a multiepitopic HIV-1 DNA vaccine by interleukin-18 DNA immunization/viral protein boost. Vaccine, 19(20-22):2803-11, 6 Apr 2001. PubMed ID: 11282190. Show all entries for this paper.

Displaying record number 609

Download this epitope record as JSON.

HXB2 Location	Tat(49-57) DNA(5975..6001)	Tat Epitope Map
Author Location	Tat(49-57)
Epitope	RKKRRQRRR	Epitope Alignment
Species (MHC/HLA)	mouse
Immunogen
Experimental methods
Keywords

Notes

• The Tat peptide RKKRRQRRR when conjugated to a protein can cause that protein to be taken up by APCs and presented to CTL.
• The system was demonstrated by vaccinating mice with an OVA-Tat peptide conjugate and immunizing H-2 K^b mice.
• The CTL response to the H-2 K^b specific OVA peptide SIINFEKL was stimulated.

References

Kim1997b D. T. Kim, D. J. Mitchell, D. G. Brockstedt, L. Fong, G. P. Nolan, C. G. Fathman, E. G. Engleman, and J. B. Rothbard. Introduction of Soluble Proteins into the MHC Class I Pathway by Conjugation to an HIV Tat Peptide. J. Immunol., 159(4):1666-1668, 1997. A vaccine based on the conjugation of OVA to an HIV Tat peptide that enhances protein uptake by APC cells stimulated MHC Class I-restricted T cell response in vitro and CTL generation in vivo in a murine system. PubMed ID: 9257826. Show all entries for this paper.

Displaying record number 58836

Download this epitope record as JSON.

HXB2 Location	Tat(49-57) DNA(5975..6001)	Tat Epitope Map
Author Location	Tat(49-57)
Epitope	RKKRRQRRR	Epitope Alignment
Epitope Name	Tat49-57
Species (MHC/HLA)	mouse
Immunogen	vaccine
Experimental methods	CD8 T-cell Elispot - IFNγ, Intracellular cytokine staining
Keywords	vaccine antigen design

Vaccine Details

Notes

• By attaching the cell penetrating peptide HIV-1 Tat_49-57 and Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF), to the immunogen, HPV16E7, a nanoparticle vaccine was able to elicit stronger CTL-dependent anti-HPV tumor activity in mice than before.

References

Tang2012 Jun Tang, Rui Yin, Yi Tian, Zeming Huang, Jinglei Shi, Xiaolan Fu, Li Wang, Yuzhang Wu, Fei Hao, and Bing Ni. A Novel Self-Assembled Nanoparticle Vaccine with HIV-1 Tat(49--57)/HPV16 E7(49--57) Fusion Peptide and GM-CSF DNA Elicits Potent and Prolonged CD8+ T Cell-Dependent Anti-Tumor Immunity in Mice. Vaccine, 30(6):1071-1082, 1 Feb 2012. PubMed ID: 22178528. Show all entries for this paper.